PMID- 28690958
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2168-8184 (Print)
IS  - 2168-8184 (Electronic)
IS  - 2168-8184 (Linking)
VI  - 9
IP  - 6
DP  - 2017 Jun 9
TI  - Application of Fluorescence In Situ Hybridization (FISH) Technique for the 
      Detection of Genetic Aberration in Medical Science.
PG  - e1325
LID - 10.7759/cureus.1325 [doi]
LID - e1325
AB  - Fluorescence in situ hybridization (FISH) is a macromolecule recognition 
      technique, which is considered as a new advent in the field of 
      cytology. Initially, it was developed as a physical mapping tool to delineate 
      genes within chromosomes. The accuracy and versatility of FISH were subsequently 
      capitalized upon in biological and medical research. This visually appealing 
      technique provides an intermediate degree of resolution between DNA analysis and 
      chromosomal investigations. FISH consists of a hybridizing DNA probe, which can 
      be labeled directly or indirectly. In the case of direct labeling, fluorescent 
      nucleotides are used, while indirect labeling is incorporated with reporter 
      molecules that are subsequently detected by fluorescent antibodies or other 
      affinity molecules. FISH is applied to detect genetic abnormalities that include 
      different characteristic gene fusions or the presence of an abnormal number of 
      chromosomes in a cell or loss of a chromosomal region or a whole chromosome. It 
      is also applied in different research applications, such as gene mapping or the 
      identification of novel oncogenes. This article reviews the concept of FISH, its 
      application, and its advantages in medical science.
FAU - Ratan, Zubair Ahmed
AU  - Ratan ZA
AD  - Department of Biomedical Engineering, Khulna University of Engineering and 
      Technology, Bangladesh.
FAU - Zaman, Sojib Bin
AU  - Zaman SB
AD  - Maternal and Child Health Division, International Centre for Diarrhoeal Disease 
      Research, Bangladesh.
FAU - Mehta, Varshil
AU  - Mehta V
AD  - Department of Internal Medicine, MGM Medical College, Navi Mumbai, India.
FAU - Haidere, Mohammad Faisal
AU  - Haidere MF
AD  - Department of Soil, Water and Environment, University of Dhaka, Bangladesh.
FAU - Runa, Nusrat Jahan
AU  - Runa NJ
AD  - Department of Biochemistry, Gazi Medical College, Khulna, Bangladesh.
FAU - Akter, Nasrin
AU  - Akter N
AD  - Medicine, Yamagata University Faculty of Medicine, Japan.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20170609
PL  - United States
TA  - Cureus
JT  - Cureus
JID - 101596737
PMC - PMC5501716
OTO - NOTNLM
OT  - fluorescence in situ hybridization
OT  - genetic anomalies
OT  - medical science
COIS- The authors have declared that no competing interests exist.
EDAT- 2017/07/12 06:00
MHDA- 2017/07/12 06:01
PMCR- 2017/06/09
CRDT- 2017/07/11 06:00
PHST- 2017/07/11 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/07/12 06:01 [medline]
PHST- 2017/06/09 00:00 [pmc-release]
AID - 10.7759/cureus.1325 [doi]
PST - epublish
SO  - Cureus. 2017 Jun 9;9(6):e1325. doi: 10.7759/cureus.1325.