PMID- 28691008
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2296-858X (Print)
IS  - 2296-858X (Electronic)
IS  - 2296-858X (Linking)
VI  - 4
DP  - 2017
TI  - Fluorescence In Situ Hybridization for Diagnosis of Whipple's Disease in 
      Formalin-Fixed Paraffin-Embedded Tissue.
PG  - 87
LID - 10.3389/fmed.2017.00087 [doi]
LID - 87
AB  - Whipple's disease (WD) is a rare chronic systemic infection with a wide range of 
      clinical symptoms, routinely diagnosed in biopsies from the small intestine and 
      other tissues by periodic acid-Schiff (PAS) diastase staining and 
      immunohistological analysis with specific antibodies. The aim of our study was to 
      improve the pathological diagnosis of WD. Therefore, we analyzed the potential of 
      fluorescence in situ hybridization (FISH) for diagnosing WD, using a Tropheryma 
      (T.) whipplei-specific probe. 19 formalin-fixed paraffin-embedded (FFPE) duodenal 
      biopsy specimens of 12 patients with treated (6/12) and untreated (6/12) WD were 
      retrospectively examined using PAS diastase staining, immunohistochemistry, and 
      FISH. 20 biopsy specimens with normal intestinal mucosa, Helicobacter pylori, or 
      mycobacterial infection, respectively, served as controls. We successfully 
      detected T. whipplei in tissue biopsies with a sensitivity of 83% in untreated 
      (5/6) and 40% in treated (4/10) cases of WD. In our study, we show that 
      FISH-based diagnosis of individual vital T. whipplei in FFPE specimens is 
      feasible and can be considered as ancillary diagnostic tool for the diagnosis of 
      WD in FFPE material. We show that FISH not only detect active WD but also be 
      helpful as an indicator for the efficiency of antibiotic treatment and for 
      detection of recurrence of disease when the signal of PAS diastase and 
      immunohistochemistry lags behind the recurrence of disease, especially if the 
      clinical course of the patient and antimicrobial treatment is considered.
FAU - Braubach, Peter
AU  - Braubach P
AD  - Institute of Pathology, Hannover Medical School (MHH), Hanover, Germany.
FAU - Lippmann, Torsten
AU  - Lippmann T
AD  - Institute of Pathology, Hannover Medical School (MHH), Hanover, Germany.
FAU - Raoult, Didier
AU  - Raoult D
AD  - Aix Marseille University, CNRS, IRD, INSERM, AP-HM, URMITE, IHU Mediterranee 
      Infection, Marseille, France.
FAU - Lagier, Jean-Christophe
AU  - Lagier JC
AD  - Aix Marseille University, CNRS, IRD, INSERM, AP-HM, URMITE, IHU Mediterranee 
      Infection, Marseille, France.
FAU - Anagnostopoulos, Ioannis
AU  - Anagnostopoulos I
AD  - Institute of Pathology, Charite Medical University Berlin, Berlin, Germany.
FAU - Zender, Steffen
AU  - Zender S
AD  - Department of Gastroenterology, Hepatology, and Endocrinology, Centre for 
      Internal Medicine, Hannover Medical School (MHH), Hanover, Germany.
FAU - Langer, Florian Peter
AU  - Langer FP
AD  - Institute of Pathology, Hannover Medical School (MHH), Hanover, Germany.
FAU - Kreipe, Hans-Heinrich
AU  - Kreipe HH
AD  - Institute of Pathology, Hannover Medical School (MHH), Hanover, Germany.
FAU - Kuhnel, Mark Philipp
AU  - Kuhnel MP
AD  - Institute of Pathology, Hannover Medical School (MHH), Hanover, Germany.
FAU - Jonigk, Danny
AU  - Jonigk D
AD  - Institute of Pathology, Hannover Medical School (MHH), Hanover, Germany.
LA  - eng
PT  - Journal Article
DEP - 20170622
PL  - Switzerland
TA  - Front Med (Lausanne)
JT  - Frontiers in medicine
JID - 101648047
PMC - PMC5479881
OTO - NOTNLM
OT  - Tropheryma whipplei
OT  - Whipple's disease
OT  - fluorescence in situ hybridization
OT  - formalin-fixed paraffin-embedded tissue
OT  - immunohistochemistry
EDAT- 2017/07/12 06:00
MHDA- 2017/07/12 06:01
PMCR- 2017/06/22
CRDT- 2017/07/11 06:00
PHST- 2017/05/08 00:00 [received]
PHST- 2017/06/07 00:00 [accepted]
PHST- 2017/07/11 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/07/12 06:01 [medline]
PHST- 2017/06/22 00:00 [pmc-release]
AID - 10.3389/fmed.2017.00087 [doi]
PST - epublish
SO  - Front Med (Lausanne). 2017 Jun 22;4:87. doi: 10.3389/fmed.2017.00087. eCollection 
      2017.