PMID- 28692662
OWN - NLM
STAT- MEDLINE
DCOM- 20170925
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Potential malignant transformation in the gastric mucosa of immunodeficient mice 
      with persistent Mycoplasma penetrans infection.
PG  - e0180514
LID - 10.1371/journal.pone.0180514 [doi]
LID - e0180514
AB  - Mycoplasma infection has been reported in immunocompromised cancer patients; 
      nevertheless, it is not clear if persistent Mycoplasma infection could facilitate 
      the proliferation of cancer cells in immunocompromised organisms. The aim of this 
      study was to examine the relationship between persistent Mycoplasma infection and 
      malignant transformation in an immunodeficient host model. Immunodeficient mouse 
      model was established using cyclophosphamide and mice gastric mucosal cells were 
      infected with Mycoplasma penetrans (Mpe). After 18 weeks, mice were sacrificed 
      and gastric mucosal Mpe infected cells were identified by fluorescence in situ 
      hybridization (FISH). Moreover, pathological and ultrastructural changes in mice 
      gastric mucosa were evaluated and the expression of multiple proto-oncogenes was 
      examined by Western blot. Our data show that Mpe infection was detected in the 
      blood of immunodeficient mice and Mpe persistent infection in mice gastric mucosa 
      was confirmed by FISH. There were pathological and ultrastructural malignant 
      transformation occurred in the gastric mucosa of infected mice compared to 
      control mice. Mpe infected mice showed lower expression of p53 and p21 and higher 
      H-ras expression compared to the control group. Moreover, expression of NF-kappaB p65 
      subunit increased in Mpe infected mice, similar to the TNF-alpha expression. Bax 
      expression in gastric mucosa of Mpe infected mice was lower while Bcl-2 
      expression was higher than in the uninfected control group. Collectively these 
      data demonstrate that persistent Mpe infection is associated with aberrant 
      expression of multiple proto-oncogenes in gastric mucosa of immunodeficient mice 
      which potentially facilitate the malignant transformation.
FAU - Cao, Shuyan
AU  - Cao S
AD  - The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China.
FAU - Shen, Dandan
AU  - Shen D
AD  - Department of Clinical Laboratory, Shanghai Tenth People's Hospital, Shanghai, 
      China.
FAU - Wang, Yadong
AU  - Wang Y
AD  - Department of Emergency, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China.
FAU - Li, Linxi
AU  - Li L
AD  - The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China.
FAU - Zhou, Liping
AU  - Zhou L
AD  - Department of Laboratory Medicine, Wenzhou Medical University, Wenzhou, Zhejiang, 
      China.
FAU - Wang, Yuxue
AU  - Wang Y
AUID- ORCID: 0000-0003-2756-9494
AD  - Department of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, 
      Hubei, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20170710
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 3.6.5.2 (ras Proteins)
SB  - IM
RIN - PLoS One. 2020 Oct 22;15(10):e0241463. PMID: 33091082
MH  - Animals
MH  - Apoptosis
MH  - Cell Transformation, Neoplastic/*pathology
MH  - Cyclin-Dependent Kinase Inhibitor p21/metabolism
MH  - Female
MH  - Gastric Mucosa/*microbiology/*pathology/ultrastructure
MH  - Mice, Inbred C57BL
MH  - Mice, SCID
MH  - Mycoplasma Infections/diagnosis/*microbiology/*pathology
MH  - Mycoplasma penetrans/*physiology/ultrastructure
MH  - NF-kappa B/metabolism
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Tumor Suppressor Protein p53/metabolism
MH  - bcl-2-Associated X Protein/metabolism
MH  - ras Proteins/metabolism
PMC - PMC5503272
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/07/12 06:00
MHDA- 2017/09/26 06:00
PMCR- 2017/07/10
CRDT- 2017/07/11 06:00
PHST- 2017/05/03 00:00 [received]
PHST- 2017/06/17 00:00 [accepted]
PHST- 2017/07/11 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/09/26 06:00 [medline]
PHST- 2017/07/10 00:00 [pmc-release]
AID - PONE-D-17-17050 [pii]
AID - 10.1371/journal.pone.0180514 [doi]
PST - epublish
SO  - PLoS One. 2017 Jul 10;12(7):e0180514. doi: 10.1371/journal.pone.0180514. 
      eCollection 2017.