PMID- 28693281
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 14
IP  - 1
DP  - 2017 Jul
TI  - Coffee reduces KRAS expression in Caco-2 human colon carcinoma cells via 
      regulation of miRNAs.
PG  - 1109-1114
LID - 10.3892/ol.2017.6227 [doi]
AB  - Previous epidemiological studies have demonstrated that moderate coffee 
      consumption is associated with a lower risk of certain types of cancer, 
      particularly colon cancer. To elucidate the molecular basis for this protective 
      action, the effect of coffee on Caco-2 human colon carcinoma cells was 
      investigated. Low concentrations of coffee (<5%) inhibited proliferation of 
      Caco-2 cells without affecting cell viability. Coffee also reduced KRAS 
      proto-oncogene, GTPase (KRAS) gene expression in a dose-dependent manner; 
      however, caffeine, caffeic acid and chlorogenic acid, three major constituents of 
      coffee, did not exhibit this effect. Increasing the duration of coffee bean 
      roasting increased the reduction in KRAS expression, suggesting that the active 
      constituents responsible for this effect emerged during the roasting process. 
      MicroRNA (miR) analysis revealed that coffee induced the expression of miR-30c 
      and miR-96, both of which target the KRAS gene. The results of the present study 
      suggested that daily coffee consumption may reduce KRAS activity, thereby 
      preventing the malignant growth of colon carcinoma cells.
FAU - Nakayama, Takuya
AU  - Nakayama T
AD  - Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, 
      Japan.
FAU - Funakoshi-Tago, Megumi
AU  - Funakoshi-Tago M
AD  - Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, 
      Japan.
FAU - Tamura, Hiroomi
AU  - Tamura H
AD  - Graduate School of Pharmaceutical Sciences, Keio University, Tokyo 105-8512, 
      Japan.
LA  - eng
PT  - Journal Article
DEP - 20170522
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5494607
OTO - NOTNLM
OT  - Caco-2
OT  - KRAS
OT  - coffee
OT  - colon cancer
OT  - miRNA
OT  - roasting
EDAT- 2017/07/12 06:00
MHDA- 2017/07/12 06:01
PMCR- 2017/05/22
CRDT- 2017/07/12 06:00
PHST- 2015/12/12 00:00 [received]
PHST- 2017/02/17 00:00 [accepted]
PHST- 2017/07/12 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/07/12 06:01 [medline]
PHST- 2017/05/22 00:00 [pmc-release]
AID - OL-0-0-6227 [pii]
AID - 10.3892/ol.2017.6227 [doi]
PST - ppublish
SO  - Oncol Lett. 2017 Jul;14(1):1109-1114. doi: 10.3892/ol.2017.6227. Epub 2017 May 
      22.