PMID- 28694500
OWN - NLM
STAT- MEDLINE
DCOM- 20190116
LR  - 20230805
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Jul 10
TI  - Resistance exercise initiates mechanistic target of rapamycin (mTOR) 
      translocation and protein complex co-localisation in human skeletal muscle.
PG  - 5028
LID - 10.1038/s41598-017-05483-x [doi]
LID - 5028
AB  - The mechanistic target of rapamycin (mTOR) is a central mediator of protein 
      synthesis in skeletal muscle. We utilized immunofluorescence approaches to study 
      mTOR cellular distribution and protein-protein co-localisation in human skeletal 
      muscle in the basal state as well as immediately, 1 and 3 h after an acute bout 
      of resistance exercise in a fed (FED; 20 g Protein/40 g carbohydrate/1 g fat) or 
      energy-free control (CON) state. mTOR and the lysosomal protein LAMP2 were highly 
      co-localised in basal samples. Resistance exercise resulted in rapid 
      translocation of mTOR/LAMP2 towards the cell membrane. Concurrently, resistance 
      exercise led to the dissociation of TSC2 from Rheb and increased in the 
      co-localisation of mTOR and Rheb post exercise in both FED and CON. In addition, 
      mTOR co-localised with Eukaryotic translation initiation factor 3 subunit F 
      (eIF3F) at the cell membrane post-exercise in both groups, with the response 
      significantly greater at 1 h of recovery in the FED compared to CON. Collectively 
      our data demonstrate that cellular trafficking of mTOR occurs in human muscle in 
      response to an anabolic stimulus, events that appear to be primarily influenced 
      by muscle contraction. The translocation and association of mTOR with positive 
      regulators (i.e. Rheb and eIF3F) is consistent with an enhanced mRNA 
      translational capacity after resistance exercise.
FAU - Song, Zhe
AU  - Song Z
AD  - School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, 
      England, UK.
FAU - Moore, Daniel R
AU  - Moore DR
AD  - Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, 
      Canada.
AD  - Human Health and Nutritional Sciences, University of Guelph, Guelph, Canada.
FAU - Hodson, Nathan
AU  - Hodson N
AD  - School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, 
      England, UK.
FAU - Ward, Carl
AU  - Ward C
AD  - Institute of Cancer and Genomic Sciences, University of Birmingham, England, UK.
FAU - Dent, Jessica R
AU  - Dent JR
AD  - School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, 
      England, UK.
FAU - O'Leary, Mary F
AU  - O'Leary MF
AD  - Institute of Inflammation and Ageing, University of Birmingham, England, UK.
FAU - Shaw, Andrew M
AU  - Shaw AM
AD  - School of Sport, University of Stirling, Scotland, UK.
FAU - Hamilton, D Lee
AU  - Hamilton DL
AD  - School of Sport, University of Stirling, Scotland, UK.
FAU - Sarkar, Sovan
AU  - Sarkar S
AD  - Institute of Cancer and Genomic Sciences, University of Birmingham, England, UK.
FAU - Gangloff, Yann-Gael
AU  - Gangloff YG
AD  - Institut NeuroMyoGene (INMG), University Lyon 1, INSERM U 1217, Lyon, France.
FAU - Hornberger, Troy A
AU  - Hornberger TA
AD  - Department of Comparative Biosciences, The University of Wisconsin, Madison, USA.
FAU - Spriet, Lawrence L
AU  - Spriet LL
AD  - Human Health and Nutritional Sciences, University of Guelph, Guelph, Canada.
FAU - Heigenhauser, George J
AU  - Heigenhauser GJ
AD  - Department of Medicine, McMaster University, Hamilton, Canada.
FAU - Philp, Andrew
AU  - Philp A
AD  - School of Sport, Exercise and Rehabilitation Sciences, University of Birmingham, 
      England, UK. a.philp@bham.ac.uk.
LA  - eng
GR  - R01 AR057347/AR/NIAMS NIH HHS/United States
GR  - R56 AR057347/AR/NIAMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170710
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Proteins)
RN  - 0 (EIF3F protein, human)
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - 0 (LAMP2 protein, human)
RN  - 0 (Lysosomal-Associated Membrane Protein 2)
RN  - 0 (RHEB protein, human)
RN  - 0 (Ras Homolog Enriched in Brain Protein)
RN  - 0 (TSC2 protein, human)
RN  - 0 (Tuberous Sclerosis Complex 2 Protein)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Adult
MH  - Cell Membrane/metabolism
MH  - Dietary Carbohydrates/administration & dosage
MH  - Dietary Proteins/administration & dosage
MH  - Eukaryotic Initiation Factor-3/*metabolism
MH  - Female
MH  - Humans
MH  - Lysosomal-Associated Membrane Protein 2/*metabolism
MH  - Male
MH  - Muscle Contraction
MH  - Muscle, Skeletal/*metabolism
MH  - Protein Binding
MH  - Protein Transport
MH  - Ras Homolog Enriched in Brain Protein/*metabolism
MH  - Resistance Training/*methods
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Tuberous Sclerosis Complex 2 Protein/*metabolism
MH  - Young Adult
PMC - PMC5504043
COIS- The authors declare that they have no competing interests.
EDAT- 2017/07/12 06:00
MHDA- 2019/01/17 06:00
PMCR- 2017/07/10
CRDT- 2017/07/12 06:00
PHST- 2015/10/07 00:00 [received]
PHST- 2017/06/19 00:00 [accepted]
PHST- 2017/07/12 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2019/01/17 06:00 [medline]
PHST- 2017/07/10 00:00 [pmc-release]
AID - 10.1038/s41598-017-05483-x [pii]
AID - 5483 [pii]
AID - 10.1038/s41598-017-05483-x [doi]
PST - epublish
SO  - Sci Rep. 2017 Jul 10;7(1):5028. doi: 10.1038/s41598-017-05483-x.