PMID- 28694521 OWN - NLM STAT- MEDLINE DCOM- 20190201 LR - 20190201 IS - 2045-2322 (Electronic) IS - 2045-2322 (Linking) VI - 7 IP - 1 DP - 2017 Jul 10 TI - Genome-wide analysis of long noncoding RNA profiling in PRRSV-infected PAM cells by RNA sequencing. PG - 4952 LID - 10.1038/s41598-017-05279-z [doi] LID - 4952 AB - Porcine reproductive and respiratory syndrome (PRRS) is a major threat to the global swine industry and causes tremendous economic losses. Its causative agent, porcine reproductive and respiratory syndrome virus (PRRSV), primarily infects immune cells, such as porcine alveolar macrophages and dendritic cells. PRRSV infection results in immune suppression, antibody-dependent enhancement, and persistent infection. Highly pathogenic strains in China cause high fever and severe inflammatory responses in the lungs. However, the pathogenesis of PRRSV is still not fully understood. In this study, we analysed the long noncoding RNA (lncRNA) and mRNA expression profiles of the HP-PRRSV GSWW15 and the North American strain FL-12 in infected porcine alveolar macrophages (PAMs) at 12 and 24 hours post-infection. We predicted 12,867 novel lncRNAs, 299 of which were differentially expressed after viral infection. The Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analyses of the genes adjacent to lncRNAs showed that they were enriched in pathways related to viral infection and immune response, indicating that lncRNAs might play regulatory roles in virus-host interactions. Our study provided information about lncRNAs in the porcine immune system and offers new insights into the pathogenic mechanism of PRRSV infection and novel antiviral therapy development. FAU - Zhang, Jing AU - Zhang J AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Sun, Pu AU - Sun P AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Gan, Lipeng AU - Gan L AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Bai, Weijie AU - Bai W AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Wang, Zhijia AU - Wang Z AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Li, Dong AU - Li D AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Cao, Yimei AU - Cao Y AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Fu, Yuanfang AU - Fu Y AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Li, Pinghua AU - Li P AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Bai, Xingwen AU - Bai X AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Ma, Xueqing AU - Ma X AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Bao, Huifang AU - Bao H AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Chen, Yingli AU - Chen Y AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. FAU - Liu, Zaixin AU - Liu Z AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. liuzaixincaas@163.com. FAU - Lu, Zengjun AU - Lu Z AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China. luzengjun@caas.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170710 PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 RN - 0 (RNA, Long Noncoding) SB - IM MH - Animals MH - Cells, Cultured MH - China MH - Gene Expression Profiling MH - Gene Expression Regulation MH - Gene Ontology MH - Gene Regulatory Networks MH - Host-Pathogen Interactions MH - Macrophages, Alveolar/chemistry/cytology/*virology MH - Porcine respiratory and reproductive syndrome virus/*pathogenicity MH - RNA, Long Noncoding/*genetics MH - Sequence Analysis, RNA/*methods MH - Swine PMC - PMC5504012 COIS- The authors declare that they have no competing interests. EDAT- 2017/07/12 06:00 MHDA- 2019/02/02 06:00 PMCR- 2017/07/10 CRDT- 2017/07/12 06:00 PHST- 2016/11/22 00:00 [received] PHST- 2017/05/26 00:00 [accepted] PHST- 2017/07/12 06:00 [entrez] PHST- 2017/07/12 06:00 [pubmed] PHST- 2019/02/02 06:00 [medline] PHST- 2017/07/10 00:00 [pmc-release] AID - 10.1038/s41598-017-05279-z [pii] AID - 5279 [pii] AID - 10.1038/s41598-017-05279-z [doi] PST - epublish SO - Sci Rep. 2017 Jul 10;7(1):4952. doi: 10.1038/s41598-017-05279-z.