PMID- 28694523
OWN - NLM
STAT- MEDLINE
DCOM- 20190115
LR  - 20190115
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Jul 10
TI  - Visualizing changes in brain-derived neurotrophic factor (BDNF) expression using 
      bioluminescence imaging in living mice.
PG  - 4949
LID - 10.1038/s41598-017-05297-x [doi]
LID - 4949
AB  - Brain-derived neurotrophic factor (BDNF) plays a fundamental role in expressing 
      various neural functions including memory consolidation. Alterations of BDNF 
      levels in the brain are associated with neurodegenerative and neuropsychiatric 
      disorders. Therefore, it is important to understand how levels of BDNF are 
      controlled. Recently we generated a novel transgenic mouse strain, termed the 
      Bdnf-Luciferase transgenic (Bdnf-Luc Tg) mouse, to monitor changes in Bdnf 
      expression. In the present study, we detected the bioluminescence signal from 
      living Bdnf-Luc Tg mice after intraperitoneal administration of d-luciferin. 
      Despite high levels of Bdnf expression in the brain, it was difficult to detect a 
      signal from the brain region, probably because of its poorly penetrable 
      (short-wavelength) bioluminescence. However, we could detect the changes in the 
      bioluminescence signal in the brain region using a luciferin analogue generating 
      a near-infrared wavelength of bioluminescence. We also found a strong correlation 
      between increases in body weight and bioluminescence signal in the abdominal 
      region of Tg mice fed a high-fat diet. These results show that changes in Bdnf 
      expression can be visualized using living mice, and that the Tg mouse could be a 
      powerful tool for clarification of the role of Bdnf expression in 
      pathophysiological and physiological conditions.
FAU - Fukuchi, Mamoru
AU  - Fukuchi M
AD  - Laboratory of Molecular Neuroscience, Faculty of Pharmacy, Takasaki University of 
      Health and Welfare, 60 Nakaorui-machi, Takasaki-shi, Gunma, 370-0033, Japan. 
      fukuchi@takasaki-u.ac.jp.
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama, 
      930-0194, Japan. fukuchi@takasaki-u.ac.jp.
FAU - Izumi, Hironori
AU  - Izumi H
AD  - Department of Molecular Neuroscience, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama, 
      930-0194, Japan.
FAU - Mori, Hisashi
AU  - Mori H
AD  - Department of Molecular Neuroscience, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama, 
      930-0194, Japan.
FAU - Kiyama, Masahiro
AU  - Kiyama M
AD  - Department of Engineering Science, Graduate School of Informatics and 
      Engineering, The University of Electro-Communications, 1-5-1 Chofugaoka, 
      Chofu-shi, Tokyo, 182-8585, Japan.
FAU - Otsuka, Satoshi
AU  - Otsuka S
AD  - Department of Engineering Science, Graduate School of Informatics and 
      Engineering, The University of Electro-Communications, 1-5-1 Chofugaoka, 
      Chofu-shi, Tokyo, 182-8585, Japan.
FAU - Maki, Shojiro
AU  - Maki S
AD  - Department of Engineering Science, Graduate School of Informatics and 
      Engineering, The University of Electro-Communications, 1-5-1 Chofugaoka, 
      Chofu-shi, Tokyo, 182-8585, Japan.
FAU - Maehata, Yosuke
AU  - Maehata Y
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama, 
      930-0194, Japan.
FAU - Tabuchi, Akiko
AU  - Tabuchi A
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama, 
      930-0194, Japan.
FAU - Tsuda, Masaaki
AU  - Tsuda M
AD  - Department of Biological Chemistry, Graduate School of Medicine and 
      Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama-shi, Toyama, 
      930-0194, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170710
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Diet, High-Fat
MH  - *Gene Expression
MH  - Genes, Reporter
MH  - *Luminescent Measurements/methods
MH  - Mice
MH  - Mice, Transgenic
MH  - *Molecular Imaging
MH  - Recombinant Fusion Proteins/genetics/metabolism
PMC - PMC5504055
COIS- The authors declare that they have no competing interests.
EDAT- 2017/07/12 06:00
MHDA- 2019/01/16 06:00
PMCR- 2017/07/10
CRDT- 2017/07/12 06:00
PHST- 2016/10/10 00:00 [received]
PHST- 2017/05/26 00:00 [accepted]
PHST- 2017/07/12 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2019/01/16 06:00 [medline]
PHST- 2017/07/10 00:00 [pmc-release]
AID - 10.1038/s41598-017-05297-x [pii]
AID - 5297 [pii]
AID - 10.1038/s41598-017-05297-x [doi]
PST - epublish
SO  - Sci Rep. 2017 Jul 10;7(1):4949. doi: 10.1038/s41598-017-05297-x.