PMID- 28694931
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1948-9358 (Print)
IS  - 1948-9358 (Electronic)
IS  - 1948-9358 (Linking)
VI  - 8
IP  - 6
DP  - 2017 Jun 15
TI  - Interleukin-18 polymorphism as an inflammatory index in metabolic syndrome: A 
      preliminary study.
PG  - 304-310
LID - 10.4239/wjd.v8.i6.304 [doi]
AB  - AIM: To assess circulatory levels of interleukin-18 (IL-18) and determine whether 
      the presence of IL-18 promoter polymorphism influences metabolic syndrome 
      phenotypes. METHODS: This study recruited one hundred and eighty individuals 
      divided into three groups with sixty subjects each as: Normal weight (18.0-22.9 
      kg/m(2)), overweight (23.0-25.9 kg/m(2)) and obese (> 26.0 kg/m(2)) according to 
      South Asian criteria of BMI. Fasting blood glucose (FBG), Lipid profile, insulin, 
      IL-18 and tumor necrosis factor (TNF)alpha were measured using ELISA kits, whereas 
      low density lipoprotein (LDL)-cholesterol, insulin resistance (HOMA-IR) and 
      insulin sensitivity (QUICKI) were calculated. The body fat percentage (BF) was 
      measured through bioelectrical impedance analysis; waist and hip circumference 
      were measured. Genotyping of IL-18 -607 C/A polymorphism was performed by using 
      tetra-primer amplification refractory mutation system. Student t test, One-way 
      analysis of variance, Hardy-Weinberg equilibrium, Pearson's chi(2) test and 
      Pearson's correlation were used, where a P value < 0.05 was considered 
      significant. RESULTS: In an aged matched study, obese subjects showed higher 
      levels of FBG, cholesterol, triglycerides and LDL levels as compared to normal 
      weight (P < 0.001). Highest levels of IL-18 and TNF levels were also seen in 
      obese subjects (IL-18: 58.87 +/- 8.59 ng/L) (TNF: 4581.93 +/- 2132.05 pg/mL). The 
      percentage of IL-18 -607 A/A polymorphism was higher in overweight and obese 
      subjects vs normal weight subjects (P < 0.001). Moreover, subjects with AA 
      genotype had a higher BF, insulin resistance, TNFalpha and IL-18 levels when compared 
      with subjects with AC (heterozygous) or CC (wild type) genotypes. However, we did 
      not find any difference in the lipid profile between three subgroups. CONCLUSION: 
      This preliminary data suggests that IL-18 polymorphism affects IL-18 levels that 
      might cause low grade inflammation, further exacerbated by increased TNFalpha. All 
      these increase the susceptibility to develop MetS. Further studies are required 
      to validate our findings.
FAU - Fatima, Syeda Sadia
AU  - Fatima SS
AD  - Syeda Sadia Fatima, Zehra Jamil, Syed Hani Abidi, Department of Biological and 
      Biomedical Sciences, Aga Khan University, Karachi, Pakistan, Karachi 74800, 
      Pakistan.
FAU - Jamil, Zehra
AU  - Jamil Z
AD  - Syeda Sadia Fatima, Zehra Jamil, Syed Hani Abidi, Department of Biological and 
      Biomedical Sciences, Aga Khan University, Karachi, Pakistan, Karachi 74800, 
      Pakistan.
FAU - Abidi, Syed Hani
AU  - Abidi SH
AD  - Syeda Sadia Fatima, Zehra Jamil, Syed Hani Abidi, Department of Biological and 
      Biomedical Sciences, Aga Khan University, Karachi, Pakistan, Karachi 74800, 
      Pakistan.
FAU - Nadeem, Daniyal
AU  - Nadeem D
AD  - Syeda Sadia Fatima, Zehra Jamil, Syed Hani Abidi, Department of Biological and 
      Biomedical Sciences, Aga Khan University, Karachi, Pakistan, Karachi 74800, 
      Pakistan.
FAU - Bashir, Zara
AU  - Bashir Z
AD  - Syeda Sadia Fatima, Zehra Jamil, Syed Hani Abidi, Department of Biological and 
      Biomedical Sciences, Aga Khan University, Karachi, Pakistan, Karachi 74800, 
      Pakistan.
FAU - Ansari, Ahmed
AU  - Ansari A
AD  - Syeda Sadia Fatima, Zehra Jamil, Syed Hani Abidi, Department of Biological and 
      Biomedical Sciences, Aga Khan University, Karachi, Pakistan, Karachi 74800, 
      Pakistan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Diabetes
JT  - World journal of diabetes
JID - 101547524
PMC - PMC5483429
OTO - NOTNLM
OT  - Body fat
OT  - High density lipoprotein
OT  - Insulin
OT  - Interleukin-18
OT  - Low density lipoprotein
OT  - Metabolic syndrome
OT  - Obesity
OT  - Polymorphism
COIS- Conflict-of-interest statement: The authors declare that they have no conflict of 
      interest.
EDAT- 2017/07/12 06:00
MHDA- 2017/07/12 06:01
PMCR- 2017/06/15
CRDT- 2017/07/12 06:00
PHST- 2017/02/28 00:00 [received]
PHST- 2017/04/03 00:00 [revised]
PHST- 2017/04/23 00:00 [accepted]
PHST- 2017/07/12 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/07/12 06:01 [medline]
PHST- 2017/06/15 00:00 [pmc-release]
AID - 10.4239/wjd.v8.i6.304 [doi]
PST - ppublish
SO  - World J Diabetes. 2017 Jun 15;8(6):304-310. doi: 10.4239/wjd.v8.i6.304.