PMID- 28695121
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 4
DP  - 2017
TI  - Abundance and Diversity of Hydrogenotrophic Microorganisms in the Infant Gut 
      before the Weaning Period Assessed by Denaturing Gradient Gel Electrophoresis and 
      Quantitative PCR.
PG  - 29
LID - 10.3389/fnut.2017.00029 [doi]
LID - 29
AB  - Delivery mode (natural vs. cesarean) and feeding type (breast vs. formula 
      feeding) are relevant factors for neonatal gut colonization. Biomolecular methods 
      have shown that the ecological structure of infant microbiota is more complex 
      than previously proposed, suggesting a relevant presence of unculturable 
      bacteria. It has also been postulated that among unculturable bacteria, 
      hydrogenotrophic populations might play a key role in infant health. 
      Sulfate-reducing bacteria (SRB), acetogens, and methanogenic archaea use 
      hydrogenotrophic pathways within the human colon. However, to date, few studies 
      have reported detection of hydrogenotrophic microorganisms in newborns, possibly 
      because of limitations on available group-specific, culture-independent 
      quantification procedures. In the present work, we analyzed 16 fecal samples of 
      healthy babies aged 1-6 months by means of quantitative PCR (qPCR) targeting the 
      16S rRNA or metabolic functional genes and by denaturing gradient gel 
      electrophoresis (DGGE). qPCR data showed quantifiable levels of methanogens, SRB, 
      and acetogens in all samples, indicating that the relative abundances of these 
      microbial groups were not affected by delivery mode (natural vs. caesarian). DGGE 
      revealed a high prevalence of the Blautia genus within the acetogenic bacteria 
      despite strong interindividual variability. Our preliminary results suggest that 
      hydrogenotrophic microorganisms, which have been a neglected group to date, 
      should be included in future ecological and metabolic studies evaluating the 
      infant intestinal microbiota.
FAU - Sagheddu, Valeria
AU  - Sagheddu V
AD  - Facolta di Scienze Agrarie, Alimentari e Ambientali, Istituto di Microbiologia, 
      Universita Cattolica del Sacro Cuore, Piacenza, Italy.
FAU - Patrone, Vania
AU  - Patrone V
AD  - Facolta di Scienze Agrarie, Alimentari e Ambientali, Istituto di Microbiologia, 
      Universita Cattolica del Sacro Cuore, Piacenza, Italy.
FAU - Miragoli, Francesco
AU  - Miragoli F
AD  - Facolta di Scienze Agrarie, Alimentari e Ambientali, Istituto di Microbiologia, 
      Universita Cattolica del Sacro Cuore, Piacenza, Italy.
FAU - Morelli, Lorenzo
AU  - Morelli L
AD  - Facolta di Scienze Agrarie, Alimentari e Ambientali, Istituto di Microbiologia, 
      Universita Cattolica del Sacro Cuore, Piacenza, Italy.
LA  - eng
PT  - Journal Article
DEP - 20170626
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC5483434
OTO - NOTNLM
OT  - Blautia
OT  - babies
OT  - gut microbiota
OT  - hydrogenotrophs
OT  - quantitative PCR
EDAT- 2017/07/12 06:00
MHDA- 2017/07/12 06:01
PMCR- 2017/01/01
CRDT- 2017/07/12 06:00
PHST- 2016/12/15 00:00 [received]
PHST- 2017/06/09 00:00 [accepted]
PHST- 2017/07/12 06:00 [entrez]
PHST- 2017/07/12 06:00 [pubmed]
PHST- 2017/07/12 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2017.00029 [doi]
PST - epublish
SO  - Front Nutr. 2017 Jun 26;4:29. doi: 10.3389/fnut.2017.00029. eCollection 2017.