PMID- 2869607 OWN - NLM STAT- MEDLINE DCOM- 19860331 LR - 20211216 IS - 0378-1135 (Print) IS - 0378-1135 (Linking) VI - 10 IP - 6 DP - 1985 Dec TI - Enterotoxigenic Escherichia coli (ETEC) and Klebsiella pneumoniae isolated from dogs with diarrhoea. PG - 577-89 AB - Faecal samples from 148 dogs with diarrhoea and from 15 healthy dogs were cultured for bacterial pathogens with enterotoxigenic properties. The aim of the study was to define the toxin profile (production of heat-labile [LT] and heat-stable [ST] toxins) and possible surface fimbrial antigens. Enterotoxigenic bacteria were isolated from 6 (4.1%) dogs with diarrhoea, four of these were Escherichia coli and two were Klebsiella pneumoniae. The E. coli strains and K. pneumoniae strains were producing both LT and ST toxins. The LT toxin from these strains was not neutralized by human anti-LT serum or anti-choleragen and did not cause coagglutination with Staphylococcus aureus coated with anti-human-LT. This suggests that the LT toxin produced by these canine isolates is non-identical to LT toxin from human strains. Three of the ETEC strains were haemagglutinating and showed surface hydrophobic properties. Electron microscopy showed that canine ETEC isolates possessed fimbriae of two different types: thick (5-5.5 nm) and thin (2-3 nm). FAU - Olson, P AU - Olson P FAU - Hedhammar, A AU - Hedhammar A FAU - Faris, A AU - Faris A FAU - Krovacek, K AU - Krovacek K FAU - Wadstrom, T AU - Wadstrom T LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 RN - 0 (Antigens, Bacterial) RN - 0 (Bacterial Toxins) RN - 0 (Enterotoxins) RN - 0 (Escherichia coli Proteins) RN - 0 (heat stable toxin (E coli)) RN - D9K3SN2LNY (heat-labile enterotoxin, E coli) SB - IM MH - Animals MH - Antigens, Bacterial MH - Bacterial Toxins/biosynthesis MH - Diarrhea/microbiology/*veterinary MH - Dog Diseases/*microbiology MH - Dogs MH - Enterotoxins/*biosynthesis MH - Escherichia coli/isolation & purification/*metabolism/ultrastructure MH - Escherichia coli Infections/microbiology/veterinary MH - *Escherichia coli Proteins MH - Feces/microbiology MH - Fimbriae, Bacterial MH - Hemagglutination MH - Klebsiella Infections/microbiology/veterinary MH - Klebsiella pneumoniae/isolation & purification/*metabolism EDAT- 1985/12/01 00:00 MHDA- 1985/12/01 00:01 CRDT- 1985/12/01 00:00 PHST- 1985/12/01 00:00 [pubmed] PHST- 1985/12/01 00:01 [medline] PHST- 1985/12/01 00:00 [entrez] AID - 0378-1135(85)90066-5 [pii] AID - 10.1016/0378-1135(85)90066-5 [doi] PST - ppublish SO - Vet Microbiol. 1985 Dec;10(6):577-89. doi: 10.1016/0378-1135(85)90066-5.