PMID- 28696557 OWN - NLM STAT- MEDLINE DCOM- 20180314 LR - 20200108 IS - 1934-8258 (Electronic) IS - 1934-8258 (Linking) VI - 94 DP - 2017 Jul 11 TI - Microscopy and Image Analysis. PG - 4.4.1-4.4.89 LID - 10.1002/cphg.42 [doi] AB - This unit provides an overview of light microscopy, including objectives, light sources, filters, film, and color photography for fluorescence microscopy and fluorescence in situ hybridization (FISH). We believe there are excellent opportunities for cytogeneticists, pathologists, and other biomedical readers, to take advantage of specimen optical clearing techniques and expansion microscopy-we briefly point to these new opportunities. (c) 2017 by John Wiley & Sons, Inc. CI - Copyright (c) 2017 John Wiley & Sons, Inc. FAU - McNamara, George AU - McNamara G AD - Biomedical Consultant, Baltimore, Maryland. FAU - Difilippantonio, Michael AU - Difilippantonio M AD - Division of Cancer Treatment and Diagnosis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland. FAU - Ried, Thomas AU - Ried T AD - Section of Cancer Genomics, Genetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland. FAU - Bieber, Frederick R AU - Bieber FR AD - Brigham and Women's Hospital, Boston, Massachusetts. LA - eng PT - Journal Article PT - Review DEP - 20170711 PL - United States TA - Curr Protoc Hum Genet JT - Current protocols in human genetics JID - 101287858 SB - IM MH - Animals MH - Color MH - Cytogenetics MH - Humans MH - In Situ Hybridization, Fluorescence MH - Microscopy/*instrumentation/*methods MH - Microscopy, Fluorescence MH - Pathology MH - Photography OTO - NOTNLM OT - digital imaging OT - fluorescence in situ hybridization OT - functional genomics OT - light microscopy EDAT- 2017/07/12 06:00 MHDA- 2018/03/15 06:00 CRDT- 2017/07/12 06:00 PHST- 2017/07/12 06:00 [entrez] PHST- 2017/07/12 06:00 [pubmed] PHST- 2018/03/15 06:00 [medline] AID - 10.1002/cphg.42 [doi] PST - epublish SO - Curr Protoc Hum Genet. 2017 Jul 11;94:4.4.1-4.4.89. doi: 10.1002/cphg.42.