PMID- 28698933
OWN - NLM
STAT- MEDLINE
DCOM- 20171101
LR  - 20181201
IS  - 1420-9071 (Electronic)
IS  - 1420-682X (Print)
IS  - 1420-682X (Linking)
VI  - 74
IP  - 23
DP  - 2017 Dec
TI  - Distinct effects on the dendritic arbor occur by microbead versus bath 
      administration of brain-derived neurotrophic factor.
PG  - 4369-4385
LID - 10.1007/s00018-017-2589-7 [doi]
AB  - Proper communication among neurons depends on an appropriately formed dendritic 
      arbor, and thus, aberrant changes to the arbor are implicated in many 
      pathologies, ranging from cognitive disorders to neurodegenerative diseases. Due 
      to the importance of dendritic shape to neuronal network function, the morphology 
      of dendrites is tightly controlled and is influenced by both intrinsic and 
      extrinsic factors. In this work, we examine how brain-derived neurotrophic 
      factor (BDNF), one of the most well-studied extrinsic regulators of dendritic 
      branching, affects the arbor when it is applied locally via microbeads to 
      cultures of hippocampal neurons. We found that local application of BDNF 
      increases both proximal and distal branching in a time-dependent manner and that 
      local BDNF application attenuates pruning of dendrites that occurs with neuronal 
      maturation. Additionally, we examined whether cytosolic PSD-95 
      interactor (cypin), an intrinsic regulator of dendritic branching, plays a role 
      in these changes and found strong evidence for the involvement of cypin in 
      BDNF-promoted increases in dendrites after 24 but not 48 h of application. This 
      current study extends our previous work in which we found that bath application 
      of BDNF for 72 h, but not shorter times, increases proximal dendrite branching 
      and that this increase occurs through transcriptional regulation of cypin. 
      Moreover, this current work illustrates how dendritic branching is regulated 
      differently by the same growth factor depending on its spatial localization, 
      suggesting a novel pathway for modulation of dendritic branching locally.
FAU - O'Neill, Kate M
AU  - O'Neill KM
AD  - Department of Cell Biology & Neuroscience, Rutgers University, 604 Allison Road, 
      Piscataway, NJ, 08854, USA.
AD  - Graduate Program in Biomedical Engineering Rutgers University, Piscataway, NJ, 
      USA.
FAU - Kwon, Munjin
AU  - Kwon M
AD  - Department of Cell Biology & Neuroscience, Rutgers University, 604 Allison Road, 
      Piscataway, NJ, 08854, USA.
FAU - Donohue, Katherine E
AU  - Donohue KE
AD  - Department of Cell Biology & Neuroscience, Rutgers University, 604 Allison Road, 
      Piscataway, NJ, 08854, USA.
FAU - Firestein, Bonnie L
AU  - Firestein BL
AUID- ORCID: 0000-0002-1679-3565
AD  - Department of Cell Biology & Neuroscience, Rutgers University, 604 Allison Road, 
      Piscataway, NJ, 08854, USA. firestein@biology.rutgers.edu.
AD  - Biomedical Engineering Graduate Faculty Rutgers University, Piscataway, NJ, USA. 
      firestein@biology.rutgers.edu.
LA  - eng
GR  - T32 GM008339/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170711
PL  - Switzerland
TA  - Cell Mol Life Sci
JT  - Cellular and molecular life sciences : CMLS
JID - 9705402
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Dendrites/*drug effects/metabolism/ultrastructure
MH  - Disks Large Homolog 4 Protein
MH  - Embryo, Mammalian
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Hippocampus/*drug effects/metabolism/ultrastructure
MH  - Intracellular Signaling Peptides and Proteins/antagonists & 
      inhibitors/*genetics/metabolism
MH  - Membrane Proteins/antagonists & inhibitors/*genetics/metabolism
MH  - Microspheres
MH  - Neuronal Plasticity/*drug effects/genetics
MH  - Primary Cell Culture
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Rats
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Transfection
PMC - PMC5819737
MID - NIHMS892285
OTO - NOTNLM
OT  - BDNF
OT  - Cytosolic PSD-95 interactor (cypin)
OT  - Dendrite arborization
OT  - Hippocampal neurons
OT  - Local stimulation
OT  - Sholl analysis
COIS- Conflict of Interest: The authors declare that they have no conflicts of 
      interest.
EDAT- 2017/07/13 06:00
MHDA- 2017/11/02 06:00
PMCR- 2018/12/01
CRDT- 2017/07/13 06:00
PHST- 2017/02/02 00:00 [received]
PHST- 2017/07/06 00:00 [accepted]
PHST- 2017/06/15 00:00 [revised]
PHST- 2017/07/13 06:00 [pubmed]
PHST- 2017/11/02 06:00 [medline]
PHST- 2017/07/13 06:00 [entrez]
PHST- 2018/12/01 00:00 [pmc-release]
AID - 10.1007/s00018-017-2589-7 [pii]
AID - 10.1007/s00018-017-2589-7 [doi]
PST - ppublish
SO  - Cell Mol Life Sci. 2017 Dec;74(23):4369-4385. doi: 10.1007/s00018-017-2589-7. 
      Epub 2017 Jul 11.