PMID- 28699024
OWN - NLM
STAT- MEDLINE
DCOM- 20180724
LR  - 20181201
IS  - 1995-8218 (Electronic)
IS  - 1673-7067 (Print)
IS  - 1995-8218 (Linking)
VI  - 33
IP  - 6
DP  - 2017 Dec
TI  - Transcriptome Sequencing Reveals Astrocytes as a Therapeutic Target in 
      Heat-Stroke.
PG  - 627-640
LID - 10.1007/s12264-017-0156-8 [doi]
AB  - Heat-stroke is a serious form of hyperthermia with high mortality, and can induce 
      severe central nervous system disorders. The neurovascular unit (NVU), which 
      consists of vascular cells, glial cells, and neurons, controls blood-brain 
      barrier (BBB) permeability and cerebral blood flow, and maintains the proper 
      functioning of neuronal circuits. However, the detailed function of each BBB 
      component in heat-stroke remains unknown. In order to interpret alterations 
      caused by heat stress, we performed transcriptome comparison of neuron and 
      astrocyte primary cultures after heat treatment. Differentially-expressed genes 
      were then selected and underwent Gene Ontology annotation and Kyoto Encyclopedia 
      of Genes and Genomes pathway analysis. Gene-act networks were also constructed, 
      and the expression of pivotal genes was validated by quantitative PCR, as well as 
      single-cell qPCR in heat-stroke rats. Our work provides valuable information on 
      the transcriptional changes in NVU cells after heat stress, reveals the diverse 
      regulatory mechanisms of two of these cellular components, and shows that a 
      cell-type-specific approach may be a promising therapeutic strategy for 
      heat-stroke treatments.
FAU - Niu, Bing
AU  - Niu B
AD  - Department of Neurology, General Hospital of Jinan Military Region, Jinan, 
      250031, China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Department of Neurology, General Hospital of Jinan Military Region, Jinan, 
      250031, China.
AD  - Department of Neurology, The 303rd Hospital of the People's Liberation Army, 
      Nanning, 530021, China.
FAU - Hu, Huaiqiang
AU  - Hu H
AD  - Department of Neurology, General Hospital of Jinan Military Region, Jinan, 
      250031, China. huhuaiqiang@126.com.
FAU - Cao, Bingzhen
AU  - Cao B
AD  - Department of Neurology, General Hospital of Jinan Military Region, Jinan, 
      250031, China. cbzxia2011@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170711
PL  - Singapore
TA  - Neurosci Bull
JT  - Neuroscience bulletin
JID - 101256850
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology
MH  - Embryo, Mammalian
MH  - Heat Stroke/genetics/*metabolism
MH  - Neurons/*metabolism
MH  - Neurovascular Coupling/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Analysis, RNA/*methods
MH  - Transcriptome/*genetics
PMC - PMC5725376
OTO - NOTNLM
OT  - Astrocyte
OT  - Heat stroke
OT  - Neuron
OT  - Primary culture
OT  - RNA-seq
OT  - Single-cell qPCR
EDAT- 2017/07/13 06:00
MHDA- 2018/07/25 06:00
PMCR- 2018/12/01
CRDT- 2017/07/13 06:00
PHST- 2017/01/17 00:00 [received]
PHST- 2017/04/24 00:00 [accepted]
PHST- 2017/07/13 06:00 [pubmed]
PHST- 2018/07/25 06:00 [medline]
PHST- 2017/07/13 06:00 [entrez]
PHST- 2018/12/01 00:00 [pmc-release]
AID - 10.1007/s12264-017-0156-8 [pii]
AID - 156 [pii]
AID - 10.1007/s12264-017-0156-8 [doi]
PST - ppublish
SO  - Neurosci Bull. 2017 Dec;33(6):627-640. doi: 10.1007/s12264-017-0156-8. Epub 2017 
      Jul 11.