PMID- 287
OWN - NLM
STAT- MEDLINE
DCOM- 19760209
LR  - 20190827
IS  - 0015-5632 (Print)
IS  - 0015-5632 (Linking)
VI  - 20
IP  - 6
DP  - 1975
TI  - Transport of 4-deoxy- and 6-deoxy-D-glucose in baker's yeast.
PG  - 496-503
AB  - Tritium-labelled 4-deoxy-D-glucose (4-dglc) and 6-deoxy-D-glucose (6-dgcl) were 
      prepared by catalytic hydrogenolysis of the corresponding deoxyiodo derivatives 
      with gaseous tritium. The two sugars are transported into Saccharomyces 
      cerevisiae by both the constitutive glucose and the inducible galactose carrier. 
      Uranyl ions are powerful inhibitors. The pH optimum in uninduced cells lies at 
      5.5 for both sugars, the apparent activation energies (between 15 and 35 degrees 
      C) are 25.1 kJ/mol and 16.5 kJ/mol, respectively. The steady-state intracellular 
      concentration of both sugars is less than the extracellular one (no uphill 
      transport). Neither of them is a substrate of yeast hexokinase. 4-Deoxy-D-glucose 
      undergoes a dinitrophenol-sensitive conversion to an unknown metabolite which is 
      not phosphorylated and may represent one of its oxidation products.
FAU - Kotyk, A
AU  - Kotyk A
FAU - Michaljanicova, D
AU  - Michaljanicova D
FAU - Veres, K
AU  - Veres K
FAU - Soukupova, V
AU  - Soukupova V
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Folia Microbiol (Praha)
JT  - Folia microbiologica
JID - 0376757
RN  - 0 (Deoxy Sugars)
RN  - 0 (Dinitrophenols)
RN  - 30237-26-4 (Fructose)
RN  - 4OC371KSTK (Uranium)
RN  - 9G2MP84A8W (Deoxyglucose)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
RN  - ZRH8M27S79 (Iodoacetamide)
SB  - IM
MH  - Deoxy Sugars/*metabolism
MH  - Deoxyglucose/chemical synthesis/*metabolism
MH  - Dinitrophenols/pharmacology
MH  - Electron Transport
MH  - Fructose/metabolism
MH  - Glucose/metabolism
MH  - Hexokinase/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Iodoacetamide/pharmacology
MH  - Saccharomyces cerevisiae/enzymology/*metabolism
MH  - Temperature
MH  - Uranium/pharmacology
EDAT- 1975/01/01 00:00
MHDA- 1975/01/01 00:01
CRDT- 1975/01/01 00:00
PHST- 1975/01/01 00:00 [pubmed]
PHST- 1975/01/01 00:01 [medline]
PHST- 1975/01/01 00:00 [entrez]
AID - 10.1007/BF02891709 [doi]
PST - ppublish
SO  - Folia Microbiol (Praha). 1975;20(6):496-503. doi: 10.1007/BF02891709.