PMID- 28701927
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 11
DP  - 2017
TI  - Activation of the CREB/c-Fos Pathway during Long-Term Synaptic Plasticity in the 
      Cerebellum Granular Layer.
PG  - 184
LID - 10.3389/fncel.2017.00184 [doi]
LID - 184
AB  - The induction of long-term potentiation and depression (LTP and LTD) is thought 
      to trigger gene expression and protein synthesis, leading to consolidation of 
      synaptic and neuronal changes. However, while LTP and LTD have been proposed to 
      play important roles for sensori-motor learning in the cerebellum granular layer, 
      their association with these mechanisms remained unclear. Here, we have 
      investigated phosphorylation of the cAMP-responsive element binding protein 
      (CREB) and activation of the immediate early gene c-Fos pathway following the 
      induction of synaptic plasticity by theta-burst stimulation (TBS) in acute 
      cerebellar slices. LTP and LTD were localized using voltage-sensitive dye imaging 
      (VSDi). At two time points following TBS (15 min and 120 min), corresponding to 
      the early and late phases of plasticity, slices were fixed and processed to 
      evaluate CREB phosphorylation (P-CREB) and c-FOS protein levels, as well as Creb 
      and c-Fos mRNA expression. High levels of P-CREB and Creb/c-Fos were detected 
      before those of c-FOS, as expected if CREB phosphorylation triggered gene 
      expression followed by protein synthesis. No differences between control slices 
      and slices stimulated with TBS were observed in the presence of an 
      N-methyl-D-aspartate receptor (NMDAR) antagonist. Interestingly, activation of 
      the CREB/c-Fos system showed a relevant degree of colocalization with long-term 
      synaptic plasticity. These results show that NMDAR-dependent plasticity at the 
      cerebellum input stage bears about transcriptional and post-transcriptional 
      processes potentially contributing to cerebellar learning and memory 
      consolidation.
FAU - Gandolfi, Daniela
AU  - Gandolfi D
AD  - Neurophysiology Unit, Department of Brain and Behavioral Sciences, University of 
      PaviaPavia, Italy.
AD  - Brain Connectivity Center, Fondazione Istituto Neurologico Nazionale Casimiro 
      Mondino (IRCCS)Pavia, Italy.
FAU - Cerri, Silvia
AU  - Cerri S
AD  - Laboratory of Functional Neurochemistry, Center for Research in Neurodegenerative 
      Diseases, Fondazione Istituto Neurologico Nazionale Casimiro Mondino 
      (IRCCS)Pavia, Italy.
FAU - Mapelli, Jonathan
AU  - Mapelli J
AD  - Neurophysiology Unit, Department of Brain and Behavioral Sciences, University of 
      PaviaPavia, Italy.
AD  - Department of Biomedical, Metabolic and Neural Sciences, Center for Neuroscience 
      and Neurotechnology, University of Modena and Reggio EmiliaModena, Italy.
FAU - Polimeni, Mariarosa
AU  - Polimeni M
AD  - Department of Public Health, Experimental and Forensic Medicine, Human Anatomy 
      Unit, University of PaviaPavia Italy.
FAU - Tritto, Simona
AU  - Tritto S
AD  - Neurophysiology Unit, Department of Brain and Behavioral Sciences, University of 
      PaviaPavia, Italy.
FAU - Fuzzati-Armentero, Marie-Therese
AU  - Fuzzati-Armentero MT
AD  - Laboratory of Functional Neurochemistry, Center for Research in Neurodegenerative 
      Diseases, Fondazione Istituto Neurologico Nazionale Casimiro Mondino 
      (IRCCS)Pavia, Italy.
FAU - Bigiani, Albertino
AU  - Bigiani A
AD  - Department of Biomedical, Metabolic and Neural Sciences, Center for Neuroscience 
      and Neurotechnology, University of Modena and Reggio EmiliaModena, Italy.
FAU - Blandini, Fabio
AU  - Blandini F
AD  - Laboratory of Functional Neurochemistry, Center for Research in Neurodegenerative 
      Diseases, Fondazione Istituto Neurologico Nazionale Casimiro Mondino 
      (IRCCS)Pavia, Italy.
FAU - Mapelli, Lisa
AU  - Mapelli L
AD  - Neurophysiology Unit, Department of Brain and Behavioral Sciences, University of 
      PaviaPavia, Italy.
AD  - Museo Storico Della Fisica e Centro Studi e Ricerche Enrico FermiRome, Italy.
FAU - D'Angelo, Egidio
AU  - D'Angelo E
AD  - Neurophysiology Unit, Department of Brain and Behavioral Sciences, University of 
      PaviaPavia, Italy.
AD  - Brain Connectivity Center, Fondazione Istituto Neurologico Nazionale Casimiro 
      Mondino (IRCCS)Pavia, Italy.
LA  - eng
PT  - Journal Article
DEP - 20170628
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC5487453
OTO - NOTNLM
OT  - CREB
OT  - NMDARs
OT  - cerebellum
OT  - gene expression
OT  - long-term plasticity
EDAT- 2017/07/14 06:00
MHDA- 2017/07/14 06:01
PMCR- 2017/01/01
CRDT- 2017/07/14 06:00
PHST- 2017/03/16 00:00 [received]
PHST- 2017/06/16 00:00 [accepted]
PHST- 2017/07/14 06:00 [entrez]
PHST- 2017/07/14 06:00 [pubmed]
PHST- 2017/07/14 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2017.00184 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2017 Jun 28;11:184. doi: 10.3389/fncel.2017.00184. 
      eCollection 2017.