PMID- 28707960
OWN - NLM
STAT- MEDLINE
DCOM- 20171127
LR  - 20181201
IS  - 1527-1315 (Electronic)
IS  - 0033-8419 (Print)
IS  - 0033-8419 (Linking)
VI  - 285
IP  - 3
DP  - 2017 Dec
TI  - Biofunctionalized Hybrid Magnetic Gold Nanoparticles as Catalysts for 
      Photothermal Ablation of Colorectal Liver Metastases.
PG  - 809-819
LID - 10.1148/radiol.2017161497 [doi]
AB  - Purpose To demonstrate that anti-MG1 conjugated hybrid magnetic gold 
      nanoparticles (HNPs) act as a catalyst during photothermal ablation (PTA) of 
      colorectal liver metastases, and thus increase ablation zones. Materials and 
      Methods All experiments were performed with approval of the institutional animal 
      care and use committee. Therapeutic and diagnostic multifunctional HNPs 
      conjugated with anti-MG1 monoclonal antibodies were synthesized, and the coupling 
      efficiency was determined. Livers of 19 Wistar rats were implanted with 5 x 10(6) 
      rat colorectal liver metastasis cell line cells. The rats were divided into three 
      groups according to injection: anti-MG1-coupled HNPs (n = 6), HNPs only (n = 6), 
      and cells only (control group, n = 7). Voxel-wise R2 and R2* magnetic resonance 
      (MR) imaging measurements were obtained before, immediately after, and 24 hours 
      after injection. PTA was then performed with a fiber-coupled near-infrared (808 
      nm) diode laser with laser power of 0.56 W/cm(2) for 3 minutes, while temperature 
      changes were measured. Tumors were assessed for necrosis with hematoxylin-eosin 
      staining. Organs were analyzed with inductively coupled plasma mass spectrometry 
      to assess biodistribution. Therapeutic efficacy and tumor necrosis area were 
      compared by using a one-way analysis of variance with post hoc analysis for 
      statistically significant differences. Results The coupling efficiency was 22 
      mug/mg (55%). Significant differences were found between preinfusion and 24-hour 
      postinfusion measurements of both T2 (repeated measures analysis of variance, P = 
      .025) and T2* (P < .001). Significant differences also existed for T2* 
      measurements between the anti-MG1 HNP and HNP-only groups (P = .034). Mean 
      temperature +/- standard deviation with PTA in the anti-MG1-coated HNP, HNP, and 
      control groups was 50.2 degrees C +/- 7.8, 51 degrees C +/- 4.4, and 39.5 degrees C +/- 2.0, respectively. 
      Inductively coupled plasma mass spectrometry revealed significant tumor targeting 
      and splenic sequestration. Mean percentages of tumor necrosis in the 
      anti-MG1-coated HNP, HNP, and control groups were 38% +/- 29, 14% +/- 17, and 7% +/- 8, 
      respectively (P = .043). Conclusion Targeted monoclonal antibody-conjugated HNPs 
      can serve as a catalyst for photothermal ablation of colorectal liver metastases 
      by increasing ablation zones. ((c)) RSNA, 2017.
FAU - White, Sarah B
AU  - White SB
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
FAU - Kim, Dong-Hyun
AU  - Kim DH
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
FAU - Guo, Yang
AU  - Guo Y
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
FAU - Li, Weiguo
AU  - Li W
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
FAU - Yang, Yihe
AU  - Yang Y
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
FAU - Chen, Jeane
AU  - Chen J
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
FAU - Gogineni, Venkateswara R
AU  - Gogineni VR
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
FAU - Larson, Andrew C
AU  - Larson AC
AD  - From the Department of Radiology, Division of Vascular and Interventional 
      Radiology, Medical College of Wisconsin, Milwaukee, Wis (S.B.W., V.R.G.); 
      Department of Radiology (S.B.W., D.H.K., Y.G., W.L., Y.Y., J.C., A.C.L.) and 
      Robert H. Lurie Comprehensive Cancer Center (D.H.K., A.C.L.), Northwestern 
      University, 710 N Fairbanks Ct, Olson 8th floor 8-317, Chicago, IL 60611; 
      Department of Chemical and Biological Engineering (J.C.) and Department of 
      Biomedical Engineering (A.C.L.), Northwestern University, Evanston, Ill.
LA  - eng
GR  - R25 CA132822/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170713
PL  - United States
TA  - Radiology
JT  - Radiology
JID - 0401260
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (MUC5B protein, human)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Mucin-5B)
RN  - 0 (Nanoconjugates)
RN  - 7440-57-5 (Gold)
SB  - IM
CIN - Radiology. 2017 Dec;285(3):699-701. PMID: 29155621
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacokinetics
MH  - Cell Line, Tumor
MH  - Colorectal Neoplasms/immunology/pathology/*therapy
MH  - Gold/*therapeutic use
MH  - Hyperthermia, Induced/methods
MH  - Liver Neoplasms/immunology/*secondary/*therapy
MH  - Low-Level Light Therapy/*methods
MH  - Magnetite Nanoparticles/*therapeutic use
MH  - Mucin-5B/immunology
MH  - Nanoconjugates/*therapeutic use
MH  - Rats
MH  - Rats, Wistar
MH  - Treatment Outcome
PMC - PMC5708290
EDAT- 2017/07/15 06:00
MHDA- 2017/11/29 06:00
PMCR- 2018/12/01
CRDT- 2017/07/15 06:00
PHST- 2017/07/15 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/07/15 06:00 [entrez]
PHST- 2018/12/01 00:00 [pmc-release]
AID - 10.1148/radiol.2017161497 [doi]
PST - ppublish
SO  - Radiology. 2017 Dec;285(3):809-819. doi: 10.1148/radiol.2017161497. Epub 2017 Jul 
      13.