PMID- 28708122
OWN - NLM
STAT- MEDLINE
DCOM- 20180416
LR  - 20240326
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 9
IP  - 7
DP  - 2017 Jul 14
TI  - Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative 
      Processes: Genotoxicity, Anti-Genotoxicity, Cytotoxicity and Apoptotic Effects.
LID - 10.3390/nu9070755 [doi]
LID - 755
AB  - Zucchini (Cucurbita pepo subsp. pepo) is a seasonal vegetable with high 
      nutritional and medical values. Many useful properties of this fruit are 
      attributed to bioactive compounds. Zucchini fruits ("Yellow" and "Light Green" 
      varieties) and four distinctive components (lutein, beta-carotene, zeaxanthin and 
      dehydroascorbic acid) were selected. Firstly, the lutein, beta-carotene, zeaxanthin 
      and dehydroascorbic acid contents were determined in these fruits. Then, in order 
      to evaluate the safety and suitability of their use, different assays were 
      carried out: (i) genotoxicity and anti-genotoxicity tests to determine the safety 
      and DNA-protection against hydrogen peroxide; (ii) cytotoxicity; and (iii) DNA 
      fragmentation and Annexin V/PI (Propidium Iodide) assays to evaluate the 
      pro-apoptotic effect. Results showed that: (i) all the substances were 
      non-genotoxic; (ii) all the substances were anti-genotoxic except the highest 
      concentration of lutein; (iii) "Yellow" zucchini epicarp and mesocarp exhibited 
      the highest cytotoxic activity (IC(50) > 0.1 mg/mL and 0.2 mg/mL, respectively); 
      and (iv) "Light Green" zucchini skin induced internucleosomal DNA fragmentation, 
      beta-carotene being the possible molecule responsible for its pro-apoptotic 
      activity. To sum up, zucchini fruit could play a positive role in human health 
      and nutrition due to this fruit and its components were safe, able to inhibit 
      significantly the H(2)O(2)-induced damage and exhibit anti-proliferative and 
      pro-apoptotic activities toward HL60 (human promyelocytic leukemia cells) tumor 
      cells. The information generated from this research should be considered when 
      selecting potential accessions for breeding program purposes.
FAU - Martinez-Valdivieso, Damian
AU  - Martinez-Valdivieso D
AD  - Department of Genomics and Biotechnology, IFAPA (Andalusian Institute of 
      Agricultural Research and Training, Fisheries, Food and Ecological Production) 
      Center La Mojonera, Camino San Nicolas, 1 La Mojonera, 04745 Almeria, Spain. 
      damianvaldivieso@gmail.com.
FAU - Font, Rafael
AU  - Font R
AD  - Department of Food and Health, IFAPA Center La Mojonera Camino San Nicolas, 1 La 
      Mojonera, 04745 Almeria, Spain. rafaelm.font@juntadeandalucia.es.
FAU - Fernandez-Bedmar, Zahira
AU  - Fernandez-Bedmar Z
AD  - Department of Genetics, University of Cordoba, Campus Rabanales, Gregor Mendel 
      Building, 14071 Cordoba, Spain. b12febez@uco.es.
FAU - Merinas-Amo, Tania
AU  - Merinas-Amo T
AD  - Department of Genetics, University of Cordoba, Campus Rabanales, Gregor Mendel 
      Building, 14071 Cordoba, Spain. tania.meram@gmail.com.
FAU - Gomez, Pedro
AU  - Gomez P
AD  - Department of Genomics and Biotechnology, IFAPA (Andalusian Institute of 
      Agricultural Research and Training, Fisheries, Food and Ecological Production) 
      Center La Mojonera, Camino San Nicolas, 1 La Mojonera, 04745 Almeria, Spain. 
      pedro.gomez.j@juntadeandalucia.es.
FAU - Alonso-Moraga, Angeles
AU  - Alonso-Moraga A
AD  - Department of Genetics, University of Cordoba, Campus Rabanales, Gregor Mendel 
      Building, 14071 Cordoba, Spain. ge1almoa@uco.es.
FAU - Del Rio-Celestino, Mercedes
AU  - Del Rio-Celestino M
AD  - Department of Genomics and Biotechnology, IFAPA (Andalusian Institute of 
      Agricultural Research and Training, Fisheries, Food and Ecological Production) 
      Center La Mojonera, Camino San Nicolas, 1 La Mojonera, 04745 Almeria, Spain. 
      mercedes.rio.celestino@juntadeandalucia.es.
LA  - eng
PT  - Journal Article
DEP - 20170714
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Antioxidants)
RN  - 0 (Mutagens)
RN  - 0 (Zeaxanthins)
RN  - 01YAE03M7J (beta Carotene)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - X72A60C9MT (Lutein)
RN  - Y2Z3ZTP9UM (Dehydroascorbic Acid)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic
MH  - Antioxidants
MH  - Apoptosis/drug effects
MH  - Cucurbita/*chemistry
MH  - DNA Damage/*drug effects
MH  - DNA Fragmentation/drug effects
MH  - Dehydroascorbic Acid/analysis/*pharmacology
MH  - Drosophila melanogaster/genetics
MH  - Fruit/chemistry
MH  - HL-60 Cells
MH  - Health Promotion
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Lutein/analysis/*pharmacology
MH  - Mutagens
MH  - Nutritive Value
MH  - Phytotherapy
MH  - Zeaxanthins/analysis/*pharmacology
MH  - beta Carotene/analysis/*pharmacology
PMC - PMC5537869
OTO - NOTNLM
OT  - Cucurbita
OT  - DNA-protection
OT  - cancer
OT  - carotenoid
OT  - chemoprevention
OT  - cytotoxicity
OT  - tumor cells
COIS- The authors declare no conflict of interest.
EDAT- 2017/07/15 06:00
MHDA- 2018/04/17 06:00
PMCR- 2017/07/01
CRDT- 2017/07/15 06:00
PHST- 2017/05/04 00:00 [received]
PHST- 2017/06/26 00:00 [revised]
PHST- 2017/07/11 00:00 [accepted]
PHST- 2017/07/15 06:00 [entrez]
PHST- 2017/07/15 06:00 [pubmed]
PHST- 2018/04/17 06:00 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - nu9070755 [pii]
AID - nutrients-09-00755 [pii]
AID - 10.3390/nu9070755 [doi]
PST - epublish
SO  - Nutrients. 2017 Jul 14;9(7):755. doi: 10.3390/nu9070755.