PMID- 28708941
OWN - NLM
STAT- MEDLINE
DCOM- 20171227
LR  - 20180319
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 95
IP  - 6
DP  - 2017 Dec
TI  - Regulation of the insulin-Akt signaling pathway and glycolysis during dehydration 
      stress in the African clawed frog Xenopus laevis.
PG  - 663-671
LID - 10.1139/bcb-2017-0117 [doi]
AB  - Estivation is an adaptive stress response utilized by some amphibians during 
      periods of drought in the summer season. In this study, we examine the regulation 
      of the insulin signaling cascade and glycolysis pathway in the African clawed 
      frog Xenopus laevis during the dehydration stress induced state of estivation. We 
      show that in the brain and heart of X. laevis, dehydration reduces the 
      phosphorylation of the insulin growth factor-1 receptor (IGF-1R), and this is 
      followed by similar reductions in the phosphorylation of the Akt and mechanistic 
      target of rapamycin (mTOR) kinase. Interestingly, phosphorylation levels of 
      IGF-1R and mTOR were not affected in the kidney, and phosphorylation levels of 
      P70S6K and the ribosomal S6 protein were elevated during dehydration stress. 
      Animals under estivation are also susceptible to periods of hypoxia, suggesting 
      that glycolysis may also be affected. We observed that protein levels of many 
      glycolytic enzymes remained unchanged during dehydration; however, the hypoxia 
      response factor-1 alpha (HIF-1alpha) protein was elevated by greater than twofold in 
      the heart during dehydration. Overall, we provide evidence that shows that the 
      insulin signaling pathway in X. laevis is regulated in a tissue-specific manner 
      during dehydration stress and suggests an important role for this signaling 
      cascade in mediating the estivation response.
FAU - Wu, Cheng-Wei
AU  - Wu CW
AD  - Institute of Biochemistry and Department of Biology, Carleton University, 1125 
      Colonel By Drive, Ottawa, K1S 5B6, Canada.
AD  - Institute of Biochemistry and Department of Biology, Carleton University, 1125 
      Colonel By Drive, Ottawa, K1S 5B6, Canada.
FAU - Tessier, Shannon N
AU  - Tessier SN
AD  - Institute of Biochemistry and Department of Biology, Carleton University, 1125 
      Colonel By Drive, Ottawa, K1S 5B6, Canada.
AD  - Institute of Biochemistry and Department of Biology, Carleton University, 1125 
      Colonel By Drive, Ottawa, K1S 5B6, Canada.
FAU - Storey, Kenneth B
AU  - Storey KB
AD  - Institute of Biochemistry and Department of Biology, Carleton University, 1125 
      Colonel By Drive, Ottawa, K1S 5B6, Canada.
AD  - Institute of Biochemistry and Department of Biology, Carleton University, 1125 
      Colonel By Drive, Ottawa, K1S 5B6, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170714
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Insulin)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Dehydration
MH  - Glycolysis
MH  - Heart
MH  - Insulin/*metabolism
MH  - Kidney/metabolism
MH  - Oxidative Stress
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - *Signal Transduction
MH  - Xenopus laevis
OTO - NOTNLM
OT  - HIF-1
OT  - desiccation
OT  - dessiccation
OT  - depression metabolique
OT  - estivation
OT  - mTOR
OT  - metabolic depression
EDAT- 2017/07/15 06:00
MHDA- 2017/12/28 06:00
CRDT- 2017/07/15 06:00
PHST- 2017/07/15 06:00 [pubmed]
PHST- 2017/12/28 06:00 [medline]
PHST- 2017/07/15 06:00 [entrez]
AID - 10.1139/bcb-2017-0117 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2017 Dec;95(6):663-671. doi: 10.1139/bcb-2017-0117. Epub 2017 
      Jul 14.