PMID- 28710013 OWN - NLM STAT- MEDLINE DCOM- 20180420 LR - 20231213 IS - 1096-1208 (Electronic) IS - 0882-4010 (Linking) VI - 110 DP - 2017 Sep TI - Murine bone marrow-derived DCs activated by porcine rotavirus stimulate the Th1 subtype response in vitro. PG - 325-334 LID - S0882-4010(16)30568-X [pii] LID - 10.1016/j.micpath.2017.07.015 [doi] AB - Rotavirus (RV) infection causes acute, watery dehydrating diarrhea and even death in infants and other young animals, resulting in a severe economic burden; however, little is known about the innate immune mechanisms associated with RV infection. Dendritic cells (DCs), which are professional antigen-presenting cells (APCs), serve as a bridge connecting the innate and adaptive immune system. In this study, the interaction between murine bone marrow-derived DCs (BMDCs) and porcine rotavirus (PRV) was investigated in vitro. Upon stimulation, the expression levels of MHC-II, CD40, CD80, CD86 and CD83 in BMDCs increased in a time-dependent manner, indicating activation and maturation by PRV. In addition, up-regulated Toll-like receptor 2 (TLR2), TLR3 and NF-kappaB increased the production of interleukin-12 and interferon-gamma. The PRV-stimulated BMDCs also showed increased stimulatory capacity in mixed lymphocyte reactions and promoted the Th1 subtype response. CI - Copyright (c) 2017 Elsevier Ltd. All rights reserved. FAU - Ye, Liping AU - Ye L AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Jiang, Yanlong AU - Jiang Y AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Yang, Guilian AU - Yang G AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Yang, Wentao AU - Yang W AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Hu, Jingtao AU - Hu J AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Cui, Yulin AU - Cui Y AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Shi, Chunwei AU - Shi C AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Liu, Jing AU - Liu J AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. FAU - Wang, Chunfeng AU - Wang C AD - College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, Jilin Agricultural University, Changchun, 130118, China. Electronic address: wangchunfeng@jlau.edu.cn. LA - eng PT - Journal Article DEP - 20170712 PL - England TA - Microb Pathog JT - Microbial pathogenesis JID - 8606191 RN - 0 (Antigens, CD) RN - 0 (B7-1 Antigen) RN - 0 (B7-2 Antigen) RN - 0 (CD40 Antigens) RN - 0 (Cytokines) RN - 0 (Immunoglobulins) RN - 0 (Membrane Glycoproteins) RN - 0 (NF-kappa B) RN - 0 (TLR3 protein, mouse) RN - 0 (Toll-Like Receptor 2) RN - 0 (Toll-Like Receptor 3) RN - 187348-17-0 (Interleukin-12) RN - 82115-62-6 (Interferon-gamma) SB - IM MH - Adaptive Immunity MH - Animals MH - Antigen-Presenting Cells/immunology/virology MH - Antigens, CD/metabolism MH - B7-1 Antigen/metabolism MH - B7-2 Antigen/metabolism MH - Bone Marrow/*immunology MH - CD40 Antigens/metabolism MH - Cytokines/genetics/metabolism MH - Dendritic Cells/cytology/*immunology/metabolism/*virology MH - Gene Expression Regulation MH - Genes, MHC Class II MH - Host-Pathogen Interactions/immunology MH - Immunity, Innate MH - Immunity, Mucosal MH - Immunoglobulins/metabolism MH - Interferon-gamma/metabolism MH - Interleukin-12/metabolism MH - Male MH - Membrane Glycoproteins/metabolism MH - Mice MH - Mice, Inbred BALB C MH - NF-kappa B/metabolism MH - Rotavirus/*immunology/pathogenicity MH - Rotavirus Infections/*immunology MH - Swine MH - Th1 Cells/*immunology MH - Toll-Like Receptor 2/genetics/metabolism MH - Toll-Like Receptor 3/genetics/metabolism MH - Up-Regulation MH - CD83 Antigen OTO - NOTNLM OT - Activation OT - Bone marrow-derived DCs (BMDCs) OT - Rotavirus EDAT- 2017/07/16 06:00 MHDA- 2018/04/21 06:00 CRDT- 2017/07/16 06:00 PHST- 2016/09/20 00:00 [received] PHST- 2016/12/18 00:00 [revised] PHST- 2017/07/10 00:00 [accepted] PHST- 2017/07/16 06:00 [pubmed] PHST- 2018/04/21 06:00 [medline] PHST- 2017/07/16 06:00 [entrez] AID - S0882-4010(16)30568-X [pii] AID - 10.1016/j.micpath.2017.07.015 [doi] PST - ppublish SO - Microb Pathog. 2017 Sep;110:325-334. doi: 10.1016/j.micpath.2017.07.015. Epub 2017 Jul 12.