PMID- 28710025 OWN - NLM STAT- MEDLINE DCOM- 20180507 LR - 20180507 IS - 1873-2747 (Electronic) IS - 0361-9230 (Linking) VI - 134 DP - 2017 Sep TI - Inhibitory effects of tetramethylpyrazine on pain transmission of trigeminal neuralgia in CCI-ION rats. PG - 72-78 LID - S0361-9230(16)30399-9 [pii] LID - 10.1016/j.brainresbull.2017.07.005 [doi] AB - Tetramethylpyrazine (TMP) has anti-inflammatory effects and is used to treat cerebral ischemic injury, but the mechanism of TMP on neural protection is not clear. Trigeminal neuralgia (TN) is a facial pain syndrome that is characterized by paroxysmal, shock-like pain attacks located in the somatosensory distribution of the trigeminal nerve. P2X3 receptor plays a crucial role in facilitating pain transmission. The present study investigates the effects of TMP on trigeminal neuralgia transmission mediated by P2X3 receptor of the trigeminal ganglia (TG). Chronic constriction injury of the infraorbital branch of the trigeminal nerve (CCI-ION) was used as a trigeminal neuralgia model. On day 15 after surgery, there was a significant decline in the mechanical hyperalgesia threshold in the territory of the ligated infraorbital nerve in the TN group, and an increase in expression of P2X3 receptor in the TG of the TN group compared with the Sham group. After treatment with TMP or A-317491, the mechanical hyperalgesia threshold of TN rats was significantly higher, and expression of P2X3 receptor in the TG noticeably declined compared with the TN group. Phosphorylation of p38 and ERK1/2 in the TN group was stronger than in the Sham group. However, the phosphorylation of p38 and ERK1/2 in the TN+TMP group and TN+A-317491 group was much lower than in the TN group. TMP significantly inhibited the ATP activated currents in HEK293 cells transfected with a P2X3 plasmid. Thus, TMP might have inhibitory effects on trigeminal neuralgia by suppressing the expression of P2X3 receptor in the TG and the phosphorylation of p38 and ERK1/2 in the TG. CI - Copyright (c) 2017 Elsevier Inc. All rights reserved. FAU - Xiong, Wei AU - Xiong W AD - Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi 330006, PR China. FAU - Tan, Mengxia AU - Tan M AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - He, Lingkun AU - He L AD - Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi 330006, PR China. FAU - Ou, Xiaoyan AU - Ou X AD - Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi 330006, PR China. FAU - Jin, Youhong AU - Jin Y AD - Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi 330006, PR China. FAU - Yang, Guo AU - Yang G AD - Queen Mary college of grade 2013, Nanchang University, Nanchang, Jiangxi 330006, PR China. FAU - Huang, Liping AU - Huang L AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - Shen, Yulin AU - Shen Y AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - Guan, Shu AU - Guan S AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - Xu, Changshui AU - Xu C AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - Li, Guilin AU - Li G AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - Liu, Shuangmei AU - Liu S AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - Xu, Hong AU - Xu H AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. FAU - Liang, Shangdong AU - Liang S AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. Electronic address: liangsd@hotmail.com. FAU - Gao, Yun AU - Gao Y AD - Department of Physiology, Medical College of Nanchang University, 461 Bayi Road, Nanchang, Jiangxi 330006, PR China. Electronic address: gaoyun90@163.com. LA - eng PT - Journal Article DEP - 20170711 PL - United States TA - Brain Res Bull JT - Brain research bulletin JID - 7605818 RN - 0 (Analgesics, Non-Narcotic) RN - 0 (Pyrazines) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Purinergic P2X3) RN - 8L70Q75FXE (Adenosine Triphosphate) RN - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) RN - V80F4IA5XG (tetramethylpyrazine) SB - IM MH - Adenosine Triphosphate/metabolism MH - Analgesics, Non-Narcotic/*pharmacology MH - Animals MH - Disease Models, Animal MH - HEK293 Cells MH - Humans MH - Hyperalgesia/drug therapy/metabolism/pathology MH - MAP Kinase Signaling System/drug effects MH - Male MH - Membrane Potentials/drug effects/physiology MH - Pain Threshold/drug effects/physiology MH - Phosphorylation/drug effects MH - Pyrazines/*pharmacology MH - RNA, Messenger/metabolism MH - Random Allocation MH - Rats, Sprague-Dawley MH - Receptors, Purinergic P2X3/genetics/metabolism MH - Trigeminal Ganglion/drug effects/metabolism/pathology MH - Trigeminal Neuralgia/*drug therapy/metabolism/pathology MH - p38 Mitogen-Activated Protein Kinases/metabolism OTO - NOTNLM OT - CCI-ION rats OT - P2X3 OT - Trigeminal ganglion OT - Trigeminal neuralgia OT - receptor EDAT- 2017/07/16 06:00 MHDA- 2018/05/08 06:00 CRDT- 2017/07/16 06:00 PHST- 2016/11/10 00:00 [received] PHST- 2017/06/04 00:00 [revised] PHST- 2017/07/06 00:00 [accepted] PHST- 2017/07/16 06:00 [pubmed] PHST- 2018/05/08 06:00 [medline] PHST- 2017/07/16 06:00 [entrez] AID - S0361-9230(16)30399-9 [pii] AID - 10.1016/j.brainresbull.2017.07.005 [doi] PST - ppublish SO - Brain Res Bull. 2017 Sep;134:72-78. doi: 10.1016/j.brainresbull.2017.07.005. Epub 2017 Jul 11.