PMID- 28713363
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Geographic Distribution of Ammonia-Oxidizing Archaea along the Kuril Islands in 
      the Western Subarctic Pacific.
PG  - 1247
LID - 10.3389/fmicb.2017.01247 [doi]
LID - 1247
AB  - Community composition and abundance of ammonia-oxidizing archaea (AOA) in the 
      ocean were affected by different physicochemical conditions, but their responses 
      to physical barriers (such as a chain of islands) were largely unknown. In our 
      study, geographic distribution of the AOA from the surface photic zone to the 
      deep bathypelagic waters in the western subarctic Pacific adjacent to the Kuril 
      Islands was investigated using pyrosequencing based on the ammonia monooxygenase 
      subunit A (amoA) gene. Genotypes of clusters A and B dominated in the upper 
      euphotic zone and the deep waters, respectively. Quantitative PCR assays revealed 
      that the occurrence and ammonia-oxidizing activity of ammonia-oxidizing archaea 
      (AOA) reached their maxima at the depth of 200 m, where a higher diversity and 
      abundance of actively transcribed AOA was observed at the station located in the 
      marginal sea exposed to more terrestrial input. Similar community composition of 
      AOA observed at the two stations adjacent to the Kuril Islands maybe due to water 
      exchange across the Bussol Strait. They distinct from the station located in the 
      western subarctic gyre, where sub-cluster WCAII had a specific distribution in 
      the surface water, and this sub-cluster seemed having a confined distribution in 
      the western Pacific. Habitat-specific groupings of different WCB sub-clusters 
      were observed reflecting the isolated microevolution existed in cluster WCB. The 
      effect of the Kuril Islands on the phylogenetic composition of AOA between the 
      Sea of Okhotsk and the western subarctic Pacific is not obvious, possibly because 
      our sampling stations are near to the Bussol Strait, the main gateway through 
      which water is exchanged between the Sea of Okhotsk and the Pacific. The vertical 
      and horizontal distribution patterns of AOA communities among stations along the 
      Kuril Islands were essentially determined by the in situ prevailing 
      physicochemical gradients along the two dimensions.
FAU - Jing, Hongmei
AU  - Jing H
AD  - CAS Key Laboratory for Experimental Study under Deep-sea Extreme Conditions, 
      Institute of Deep-sea Science and Engineering, Chinese Academy of SciencesSanya, 
      China.
FAU - Cheung, Shunyan
AU  - Cheung S
AD  - Division of Life Science, The Hong Kong University of Science and 
      TechnologyKowloon, China.
FAU - Xia, Xiaomin
AU  - Xia X
AD  - Division of Life Science, The Hong Kong University of Science and 
      TechnologyKowloon, China.
FAU - Suzuki, Koji
AU  - Suzuki K
AD  - Faculty of Environmental Earth Science, Hokkaido UniversitySapporo, Japan.
FAU - Nishioka, Jun
AU  - Nishioka J
AD  - Institute of Low Temperature Science, Hokkaido UniversitySapporo, Japan.
FAU - Liu, Hongbin
AU  - Liu H
AD  - Division of Life Science, The Hong Kong University of Science and 
      TechnologyKowloon, China.
LA  - eng
PT  - Journal Article
DEP - 20170630
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5492448
OTO - NOTNLM
OT  - AOA
OT  - amoA
OT  - geographic distribution
OT  - pyrosequencing
EDAT- 2017/07/18 06:00
MHDA- 2017/07/18 06:01
PMCR- 2017/06/30
CRDT- 2017/07/18 06:00
PHST- 2017/02/15 00:00 [received]
PHST- 2017/06/20 00:00 [accepted]
PHST- 2017/07/18 06:00 [entrez]
PHST- 2017/07/18 06:00 [pubmed]
PHST- 2017/07/18 06:01 [medline]
PHST- 2017/06/30 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.01247 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Jun 30;8:1247. doi: 10.3389/fmicb.2017.01247. eCollection 
      2017.