PMID- 28713579
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2053-1095 (Print)
IS  - 2053-1095 (Electronic)
IS  - 2053-1095 (Linking)
VI  - 3
IP  - 2
DP  - 2017 May
TI  - Ring trial 2016 for Bluetongue virus detection by real-time RT-PCR in France.
PG  - 107-114
LID - 10.1002/vms3.63 [doi]
AB  - Since the unexpected emergence of BTV-8 in Northern Europe and the incursion of 
      BTV-8 and 1 in France in 2006-2007, molecular diagnosis has considerably evolved. 
      Several real-time RT-PCR (rtRT-PCR) methods have been developed and published, 
      and are currently being used in many countries across Europe for BTV detection 
      and typing. In France, the national reference laboratory (NRL) for orbiviruses 
      develops and validates 'ready-to-use' kits with private companies for viral RNA 
      detection. The regional laboratories network that was set up to deal with a heavy 
      demand for analyses has used these available kits. From 2007, ring tests were 
      organized to monitor the performance of the French laboratories. This study 
      presents the results of 63 regional laboratories in the ring trial organized in 
      2016. Blood samples were sent to the laboratories. Participants were asked to use 
      the rtRT-PCR methods in place in their laboratory, for detection of all BTV 
      serotypes and specifically BTV-8. The French regional laboratories are able to 
      detect and genotype BTV in affected animals. Despite the use of several methods 
      (i.e. RNA extraction and different commercial rtRT-PCRs), the network is 
      homogeneous. The ring trial demonstrated that the French regional veterinary 
      laboratories have reliable and robust BTV diagnostic tools for BTV genome 
      detection.
FAU - Sailleau, Corinne
AU  - Sailleau C
AUID- ORCID: 0000-0001-9077-6934
AD  - ANSES/INRA/ENVA-UPECUMR 1161 Virologie14 rue Pierre et Marie CURIE-94700Maisons 
      AlfortFrance.
FAU - Viarouge, Cyril
AU  - Viarouge C
AD  - ANSES/INRA/ENVA-UPECUMR 1161 Virologie14 rue Pierre et Marie CURIE-94700Maisons 
      AlfortFrance.
FAU - Breard, Emmanuel
AU  - Breard E
AD  - ANSES/INRA/ENVA-UPECUMR 1161 Virologie14 rue Pierre et Marie CURIE-94700Maisons 
      AlfortFrance.
FAU - Vitour, Damien
AU  - Vitour D
AD  - ANSES/INRA/ENVA-UPECUMR 1161 Virologie14 rue Pierre et Marie CURIE-94700Maisons 
      AlfortFrance.
FAU - Zientara, Stephan
AU  - Zientara S
AD  - ANSES/INRA/ENVA-UPECUMR 1161 Virologie14 rue Pierre et Marie CURIE-94700Maisons 
      AlfortFrance.
LA  - eng
PT  - Journal Article
DEP - 20170416
PL  - England
TA  - Vet Med Sci
JT  - Veterinary medicine and science
JID - 101678837
PMC - PMC5488199
OTO - NOTNLM
OT  - Bluetongue
OT  - diagnosis
OT  - real-time RT-PCR
OT  - ring trial
EDAT- 2017/07/18 06:00
MHDA- 2017/07/18 06:01
PMCR- 2017/04/16
CRDT- 2017/07/18 06:00
PHST- 2016/11/07 00:00 [received]
PHST- 2017/02/24 00:00 [revised]
PHST- 2017/02/28 00:00 [accepted]
PHST- 2017/07/18 06:00 [entrez]
PHST- 2017/07/18 06:00 [pubmed]
PHST- 2017/07/18 06:01 [medline]
PHST- 2017/04/16 00:00 [pmc-release]
AID - VMS363 [pii]
AID - 10.1002/vms3.63 [doi]
PST - epublish
SO  - Vet Med Sci. 2017 Apr 16;3(2):107-114. doi: 10.1002/vms3.63. eCollection 2017 
      May.