PMID- 28713889
OWN - NLM
STAT- MEDLINE
DCOM- 20180430
LR  - 20211204
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 3
DP  - 2017 Sep
TI  - TFE3 regulates renal adenocarcinoma cell proliferation via activation of the mTOR 
      pathway.
PG  - 2721-2725
LID - 10.3892/mmr.2017.6930 [doi]
AB  - The present study aimed to investigate the role of transcription factor E3 (TFE3) 
      in the regulation of proliferation in renal adenocarcinoma cells. The LV‑TFE3 
      overexpression (OE) lentivirus and negative control CON195 (NC) lentivirus were 
      transfected into the ACHN cell line. Protein expression of FLAG‑tag TFE3 was 
      determined using western blot analysis. Differences in cell proliferation, plate 
      clone formation and cell cycle distribution between OE and NC groups were 
      compared using MTT, plate colony formation and flow cytometry assays, 
      respectively. The levels of mammalian target of rapamycin (mTOR) and 
      phosphorylated ribosomal protein S6 (p‑rpS6) were analyzed by western blotting. 
      Cell proliferation and colony formation increased significantly in the OE group 
      compared with the NC group. The % of cells in the G1 and G2 phases of the cell 
      cycle decreased, while the % of cells in the S phase of the cell cycle increased 
      in the OE group compared with the NC group. In addition, mTOR and p‑rpS6 levels 
      were increased in the OE group compared with the NC group. The results of the 
      present study demonstrated that TFE3 overexpression resulted in increased ACHN 
      cell proliferation and plate clone formation. TFE3 may promote renal tumor growth 
      by regulating cell cycle progression and activating the phosphatidylinositol 
      3‑kinase/AKT serine/threonine kinase 1/mTOR signaling pathway.
FAU - Fang, Yuan
AU  - Fang Y
AD  - Reproductive Medical Center, Jinling Hospital, Clinical Medical School of 
      Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
FAU - Bao, Wei
AU  - Bao W
AD  - Department of Pathology, Jinling Hospital, Clinical Medical School of Southern 
      Medical University, Nanjing, Jiangsu 210002, P.R. China.
FAU - Rao, Qiu
AU  - Rao Q
AD  - Department of Pathology, Jinling Hospital, Clinical Medical School of Southern 
      Medical University, Nanjing, Jiangsu 210002, P.R. China.
FAU - Wang, Xuan
AU  - Wang X
AD  - Department of Pathology, Jinling Hospital, Clinical Medical School of Southern 
      Medical University, Nanjing, Jiangsu 210002, P.R. China.
FAU - Xia, Qiuyuan
AU  - Xia Q
AD  - Department of Pathology, Jinling Hospital, Clinical Medical School of Southern 
      Medical University, Nanjing, Jiangsu 210002, P.R. China.
FAU - Shen, Qin
AU  - Shen Q
AD  - Department of Pathology, Jinling Hospital, Clinical Medical School of Southern 
      Medical University, Nanjing, Jiangsu 210002, P.R. China.
FAU - Zhou, Xiaojun
AU  - Zhou X
AD  - Department of Pathology, Jinling Hospital, Clinical Medical School of Southern 
      Medical University, Nanjing, Jiangsu 210002, P.R. China.
FAU - Yao, Bing
AU  - Yao B
AD  - Reproductive Medical Center, Jinling Hospital, Clinical Medical School of 
      Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170705
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (TFE3 protein, human)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics/*metabolism
MH  - Carcinoma, Renal Cell/genetics/*metabolism/pathology
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - *Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Kidney Neoplasms/genetics/*metabolism/pathology
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Up-Regulation
PMC - PMC5547915
EDAT- 2017/07/18 06:00
MHDA- 2018/05/01 06:00
PMCR- 2017/07/05
CRDT- 2017/07/18 06:00
PHST- 2016/08/17 00:00 [received]
PHST- 2017/05/15 00:00 [accepted]
PHST- 2017/07/18 06:00 [pubmed]
PHST- 2018/05/01 06:00 [medline]
PHST- 2017/07/18 06:00 [entrez]
PHST- 2017/07/05 00:00 [pmc-release]
AID - mmr-16-03-2721 [pii]
AID - 10.3892/mmr.2017.6930 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Sep;16(3):2721-2725. doi: 10.3892/mmr.2017.6930. Epub 2017 Jul 
      5.