PMID- 28715454
OWN - NLM
STAT- MEDLINE
DCOM- 20170927
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Reduction of Cav1.3 channels in dorsal hippocampus impairs the development of 
      dentate gyrus newborn neurons and hippocampal-dependent memory tasks.
PG  - e0181138
LID - 10.1371/journal.pone.0181138 [doi]
LID - e0181138
AB  - Cav1.3 has been suggested to mediate hippocampal neurogenesis of adult mice and 
      contribute to hippocampal-dependent learning and memory processes. However, the 
      mechanism of Cav1.3 contribution in these processes is unclear. Here, roles of 
      Cav1.3 of mouse dorsal hippocampus during newborn cell development were examined. 
      We find that knock-out (KO) of Cav1.3 resulted in the reduction of survival of 
      newborn neurons at 28 days old after mitosis. The retroviral eGFP expression 
      showed that both dendritic complexity and the number and length of mossy fiber 
      bouton (MFB) filopodia of newborn neurons at >/= 14 days old were significantly 
      reduced in KO mice. Both contextual fear conditioning (CFC) and object-location 
      recognition tasks were impaired in recent (1 day) memory test while passive 
      avoidance task was impaired only in remote (>/= 20 days) memory in KO mice. Results 
      using adeno-associated virus (AAV)-mediated Cav1.3 knock-down (KD) or 
      retrovirus-mediated KD in dorsal hippocampal DG area showed that the recent 
      memory of CFC was impaired in both KD mice but the remote memory was impaired 
      only in AAV KD mice, suggesting that Cav1.3 of mature neurons play important 
      roles in both recent and remote CFC memory while Cav1.3 in newborn neurons is 
      selectively involved in the recent CFC memory process. Meanwhile, AAV KD of 
      Cav1.3 in ventral hippocampal area has no effect on the recent CFC memory. In 
      conclusion, the results suggest that Cav1.3 in newborn neurons of dorsal 
      hippocampus is involved in the survival of newborn neurons while mediating 
      developments of dendritic and axonal processes of newborn cells and plays a role 
      in the memory process differentially depending on the stage of maturation and the 
      type of learning task.
FAU - Kim, Su-Hyun
AU  - Kim SH
AD  - Center for Neuroscience, Korea Institute of Science and Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology, Seoul, Korea.
FAU - Park, Ye-Ryoung
AU  - Park YR
AD  - Center for Neuroscience, Korea Institute of Science and Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology, Seoul, Korea.
FAU - Lee, Boyoung
AU  - Lee B
AD  - Center for Cognition and Sociality, Institute for Basic Science, Daejeon, Korea.
FAU - Choi, Byungil
AU  - Choi B
AD  - Department of Anatomy and Division of Brain Korea 21 Biomedical Science, College 
      of Medicine, Korea University, Seoul, Korea.
FAU - Kim, Hyun
AU  - Kim H
AD  - Department of Anatomy and Division of Brain Korea 21 Biomedical Science, College 
      of Medicine, Korea University, Seoul, Korea.
FAU - Kim, Chong-Hyun
AU  - Kim CH
AUID- ORCID: 0000-0002-0968-4176
AD  - Center for Neuroscience, Korea Institute of Science and Technology, Seoul, Korea.
AD  - Neuroscience Program, Division of Bio-Medical Science and Technology, KIST 
      School, Korea University of Science and Technology, Seoul, Korea.
LA  - eng
PT  - Journal Article
DEP - 20170717
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cacna1d protein, mouse)
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Calcium Channels, L-Type/chemistry/genetics/*metabolism
MH  - Dendrites/physiology
MH  - Dentate Gyrus/growth & development/*metabolism/pathology
MH  - Dependovirus/genetics
MH  - Fear
MH  - Genetic Vectors/metabolism
MH  - Hippocampus/*metabolism/pathology
MH  - Male
MH  - Memory/physiology
MH  - Memory, Long-Term
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Microscopy, Confocal
MH  - Neurons/*metabolism
MH  - Pseudopodia/physiology
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Retroviridae/genetics
PMC - PMC5513490
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/07/18 06:00
MHDA- 2017/09/28 06:00
PMCR- 2017/07/17
CRDT- 2017/07/18 06:00
PHST- 2017/03/18 00:00 [received]
PHST- 2017/06/27 00:00 [accepted]
PHST- 2017/07/18 06:00 [entrez]
PHST- 2017/07/18 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2017/07/17 00:00 [pmc-release]
AID - PONE-D-17-10715 [pii]
AID - 10.1371/journal.pone.0181138 [doi]
PST - epublish
SO  - PLoS One. 2017 Jul 17;12(7):e0181138. doi: 10.1371/journal.pone.0181138. 
      eCollection 2017.