PMID- 28719653
OWN - NLM
STAT- MEDLINE
DCOM- 20170927
LR  - 20201214
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Isolation of a monoclonal antibody from a phage display library binding the 
      rhesus macaque MHC class I allomorph Mamu-A1*001.
PG  - e0179039
LID - 10.1371/journal.pone.0179039 [doi]
LID - e0179039
AB  - Monoclonal antibodies that bind to human leukocyte antigen (HLA) are useful tools 
      for HLA-typing, tracking donor-recipient chimerisms after bone marrow 
      transplants, and characterizing specific major histocompatibility complexes (MHC) 
      on cell surfaces. Unfortunately, equivalent reagents are not available for rhesus 
      macaques, which are commonly used animal as models in organ transplant and 
      infectious disease research. To address this deficiency, we isolated an antibody 
      that recognizes the common Indian rhesus macaque MHC class I molecule, 
      Mamu-A1*001. We induced Mamu-A1*001-binding antibodies by alloimmunizing a female 
      Mamu-A1*001-negative rhesus macaque with peripheral blood mononuclear cells 
      (PBMC) from a male Mamu-A1*001-positive donor. A Fab phage display library was 
      constructed with PBMC from the alloimmunized macaque and panned to isolate an 
      antibody that binds to Mamu-A1*001 but not to other common rhesus macaque MHC 
      class I molecules. The isolated antibody distinguishes PBMC from 
      Mamu-A1*001-positive and -negative macaques. Additionally, the 
      Mamu-A1*001-specific antibody binds the cynomolgus macaque MHC class I ortholog 
      Mafa-A1*001:01 but not variants Mafa-A1*001:02/03, indicating a high degree of 
      binding specificity. The Mamu-A1*001-specific antibody will be useful for 
      identifying Mamu-A1*001-positive rhesus macaques, for detecting 
      Mamu-A1*001-positive cells in populations of Mamu-A1*001-negative cells, and for 
      examining disease processes that alter expression of Mamu-A1*001 on cell 
      surfaces. Moreover, the alloimmunization process we describe will be useful for 
      isolating additional MHC allomorph-specific monoclonal antibodies or antibodies 
      against other polymorphic host proteins which are difficult to isolate with 
      traditional technologies.
FAU - Holman, Nathan
AU  - Holman N
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, Wisconsin, United States of America.
FAU - Weinfurter, Jason T
AU  - Weinfurter JT
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, Wisconsin, United States of America.
FAU - Harsla, Trevor R
AU  - Harsla TR
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, Wisconsin, United States of America.
FAU - Wiseman, Roger W
AU  - Wiseman RW
AD  - Wisconsin National Primate Research Center, University of Wisconsin-Madison, 
      Madison, Wisconsin, United States of America.
FAU - Belli, Aaron J
AU  - Belli AJ
AD  - MassBiologics, University of Massachusetts Medical School, Boston, Massachusetts, 
      United States of America.
FAU - Michaels, Anthony J
AU  - Michaels AJ
AD  - MassBiologics, University of Massachusetts Medical School, Boston, Massachusetts, 
      United States of America.
FAU - Reimann, Keith A
AU  - Reimann KA
AD  - MassBiologics, University of Massachusetts Medical School, Boston, Massachusetts, 
      United States of America.
FAU - DeMars, Robert I
AU  - DeMars RI
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, Wisconsin, United States of America.
FAU - Reynolds, Matthew R
AU  - Reynolds MR
AUID- ORCID: 0000-0002-1484-6408
AD  - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 
      Madison, Wisconsin, United States of America.
AD  - Wisconsin National Primate Research Center, University of Wisconsin-Madison, 
      Madison, Wisconsin, United States of America.
LA  - eng
GR  - HHSN272201100013I/AI/NIAID NIH HHS/United States
GR  - HHSN272201600007C/AI/NIAID NIH HHS/United States
GR  - HHSN272201100013C/AI/NIAID NIH HHS/United States
GR  - C06 RR020141/RR/NCRR NIH HHS/United States
GR  - C06 RR015459/RR/NCRR NIH HHS/United States
PT  - Journal Article
DEP - 20170718
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptide Library)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology/*isolation & purification
MH  - Female
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Humans
MH  - Immunization
MH  - Macaca mulatta
MH  - Male
MH  - *Peptide Library
PMC - PMC5515393
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/07/19 06:00
MHDA- 2017/09/28 06:00
PMCR- 2017/07/18
CRDT- 2017/07/19 06:00
PHST- 2017/03/24 00:00 [received]
PHST- 2017/05/23 00:00 [accepted]
PHST- 2017/07/19 06:00 [entrez]
PHST- 2017/07/19 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2017/07/18 00:00 [pmc-release]
AID - PONE-D-17-11624 [pii]
AID - 10.1371/journal.pone.0179039 [doi]
PST - epublish
SO  - PLoS One. 2017 Jul 18;12(7):e0179039. doi: 10.1371/journal.pone.0179039. 
      eCollection 2017.