PMID- 28719814
OWN - NLM
STAT- MEDLINE
DCOM- 20180201
LR  - 20181202
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 145
DP  - 2017 Oct 25
TI  - Enantiomeric separation of seven beta-agonists by NACE-Study of chiral selectivity 
      with diacetone-d-mannitol-boric acid complex.
PG  - 399-405
LID - S0731-7085(17)30743-4 [pii]
LID - 10.1016/j.jpba.2017.06.044 [doi]
AB  - A rapid and effective nonaqueous capillary electrophoresis (NACE)-ultraviolet 
      (UV) method was developed for the enantiomeric separation of seven beta-agonists. 
      Diacetone-d-mannitol-boric acid complex was used as a new chiral selector. It was 
      in situ synthesized by the reaction of diacetone-d-mannitol and boric acid in 
      methanol medium containing triethylamine. The effects of diacetone-d-mannitol, 
      boric acid, and triethylamine concentrations on the enantioseparation were 
      carefully investigated. Under the optimized conditions, baseline 
      enantioseparation could be obtained for six of the tested beta-agonists within 
      12min. These results were better than that obtained with d-mannitol-boric acid 
      complex in previous work. (11)B nuclear magnetic resonance ((11)B NMR) was 
      applied to determine the fraction of boron species and confirm the formation of 
      diacetone-d-mannitol-boric acid complex. Validation of the established NACE 
      method was also carried out according to ICH guidelines. Calibration curves 
      showed good linearity with correlation coefficients (r)>/=0.9992 over a certain 
      concentration range for each enantiomer of the tested five beta-agonists. The 
      relative standard deviations (RSDs) of intra-day precisions and inter-day 
      precisions of migration times were </=1.4% (n=6), and </=6.3% (n=10), respectively. 
      That of peak areas were </=3.7% (n=6), and </=5.6% (n=10), respectively. The limits 
      of detection (LODs) and the limits of quantitation (LOQs) based on the 
      signal-to-noise ratios of 3 and 10 were found below 1.25mugmL(-1) and 
      5.00mugmL(-1), respectively. The proposed method was successfully applied to the 
      determination of clenbuterol enantiomers in a multi-component pharmaceutical 
      dosage form called "Ambroxol Hydrochloride and Clenbuterol Hydrochloride Oral 
      Solution".
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Lv, Lili
AU  - Lv L
AD  - Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of 
      Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory 
      of Medical Chemistry and Molecular Diagnosis, Ministry of Education, Hebei 
      University, Baoding 071002, China.
FAU - Wang, Lijuan
AU  - Wang L
AD  - Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of 
      Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory 
      of Medical Chemistry and Molecular Diagnosis, Ministry of Education, Hebei 
      University, Baoding 071002, China. Electronic address: wanglijuan@hbu.edu.cn.
FAU - Li, Jun
AU  - Li J
AD  - Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of 
      Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory 
      of Medical Chemistry and Molecular Diagnosis, Ministry of Education, Hebei 
      University, Baoding 071002, China.
FAU - Jiao, Yajun
AU  - Jiao Y
AD  - Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of 
      Pharmaceutical Sciences, Hebei University, Baoding 071002, China; Key Laboratory 
      of Medical Chemistry and Molecular Diagnosis, Ministry of Education, Hebei 
      University, Baoding 071002, China.
FAU - Gao, Shengnan
AU  - Gao S
AD  - Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of 
      Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
FAU - Wang, Jiachang
AU  - Wang J
AD  - Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of 
      Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
FAU - Yan, Hongyuan
AU  - Yan H
AD  - Key Laboratory of Medical Chemistry and Molecular Diagnosis, Ministry of 
      Education, Hebei University, Baoding 071002, China.
LA  - eng
PT  - Journal Article
DEP - 20170708
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Boric Acids)
RN  - 3OWL53L36A (Mannitol)
SB  - IM
MH  - Boric Acids/*chemistry
MH  - Electrophoresis, Capillary
MH  - Mannitol
MH  - Signal-To-Noise Ratio
MH  - Stereoisomerism
OTO - NOTNLM
OT  - (11)B Nuclear magnetic resonance
OT  - Beta-agonist
OT  - Chiral selector
OT  - Diacetone-d-mannitol-boric acid complex
OT  - Method validation
OT  - Nonaqueous capillary electrophoresis
EDAT- 2017/07/19 06:00
MHDA- 2018/02/02 06:00
CRDT- 2017/07/19 06:00
PHST- 2017/03/24 00:00 [received]
PHST- 2017/06/13 00:00 [revised]
PHST- 2017/06/17 00:00 [accepted]
PHST- 2017/07/19 06:00 [pubmed]
PHST- 2018/02/02 06:00 [medline]
PHST- 2017/07/19 06:00 [entrez]
AID - S0731-7085(17)30743-4 [pii]
AID - 10.1016/j.jpba.2017.06.044 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2017 Oct 25;145:399-405. doi: 10.1016/j.jpba.2017.06.044. 
      Epub 2017 Jul 8.