PMID- 28719910
OWN - NLM
STAT- MEDLINE
DCOM- 20170929
LR  - 20170929
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 152
IP  - 2
DP  - 2017
TI  - Correlating the Genetic and Physical Map of Barley Chromosome 3H Revealed 
      Limitations of the FISH-Based Mapping of Nearby Single-Copy Probes Caused by the 
      Dynamic Structure of Metaphase Chromosomes.
PG  - 90-96
LID - 10.1159/000478631 [doi]
AB  - Genetic maps are based on the recombination frequency of molecular markers which 
      often show different positions in comparison to the corresponding physical maps. 
      To decipher the position and order of DNA sequences genetically mapped to 
      terminal and interstitial regions of barley (Hordeum vulgare) chromosome 3H, 
      fluorescence in situ hybridization (FISH) on mitotic metaphase chromosomes was 
      performed with 16 genomic single-copy probes derived from fingerprinted BAC 
      contigs. Long genetic distances at subterminal regions translated into short 
      physical distances, confirming that recombination events occur more often at 
      distal regions of chromosome 3H. Nonoverlapping FISH signals were frequently 
      obtained for probes with a physical distance of at least 30-60 kb. Only 8% of the 
      analyzed chromosomes showed a symmetric order of FISH signals on both sister 
      chromatids. Due to the dynamic packing of metaphase chromatin, the order of 2 
      adjacent single-copy signals along the chromosome arms outside the 
      (peri)centromeric region can only reliably be determined if the cytological 
      distance is approximately 3%, corresponding to 21.6 Mb.
CI  - (c) 2017 S. Karger AG, Basel.
FAU - Bustamante, Fernanda O
AU  - Bustamante FO
AD  - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 
      Seeland, Germany.
FAU - Aliyeva-Schnorr, Lala
AU  - Aliyeva-Schnorr L
FAU - Fuchs, Jorg
AU  - Fuchs J
FAU - Beier, Sebastian
AU  - Beier S
FAU - Houben, Andreas
AU  - Houben A
LA  - eng
PT  - Journal Article
DEP - 20170719
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Base Pairing/genetics
MH  - Chromatids/genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Plant/*genetics
MH  - *Gene Dosage
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Metaphase/*genetics
MH  - Physical Chromosome Mapping/*methods
OTO - NOTNLM
OT  - Chromosome structure
OT  - Metaphase chromosome
OT  - Physical mapping
OT  - Single-copy FISH
EDAT- 2017/07/19 06:00
MHDA- 2017/09/30 06:00
CRDT- 2017/07/19 06:00
PHST- 2017/05/03 00:00 [accepted]
PHST- 2017/07/19 06:00 [pubmed]
PHST- 2017/09/30 06:00 [medline]
PHST- 2017/07/19 06:00 [entrez]
AID - 000478631 [pii]
AID - 10.1159/000478631 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2017;152(2):90-96. doi: 10.1159/000478631. Epub 2017 Jul 
      19.