PMID- 28719954
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20181113
IS  - 1738-0006 (Electronic)
IS  - 0023-4001 (Print)
IS  - 0023-4001 (Linking)
VI  - 55
IP  - 3
DP  - 2017 Jun
TI  - Subsets of Inflammatory Cytokine Gene Polymorphisms are Associated with Risk of 
      Carcinogenic Liver Fluke Opisthorchis viverrini-Associated Advanced Periductal 
      Fibrosis and Cholangiocarcinoma.
PG  - 295-304
LID - 10.3347/kjp.2017.55.3.295 [doi]
AB  - Opisthorchis viverrini infection induces chronic inflammation, and a minor 
      proportion of infected individuals develop advanced periductal fibrosis (APF) and 
      cholangiocarcinoma (CCA). Inflammatory cytokines and/or their gene polymorphisms 
      may link to these biliary pathologies. We therefore investigated associations 
      among cytokine gene polymorphisms and cytokine production in 510 Thai cases 
      infected with O. viverrini who presented with APF+ or APF-, as established by 
      abdominal ultrasonography as well as in patients diagnosed with CCA. Levels of 
      pro-inflammatory and anti-inflammatory cytokines were determined in culture 
      supernatants after stimulation of peripheral blood mononuclear cells (PBMCs) with 
      O. viverrini excretory-secretory (ES) products. Pro-inflammatory cytokines, 
      IL-1beta, IL-6, IFN-gamma, LT-alpha, and TNF-alpha were significantly increased in CCA patients 
      compared with non-CCA (APF- and APF+) cases. Polymorphisms in genes encoding 
      IL-1beta-511C/T, IL-6-174G/C, IFN-gamma +874T/A, LT-alpha +252A/G, and TNF-alpha -308G/A were 
      then investigated by using PCR-RFLP or allele specific-PCR (AS-PCR) analyses. In 
      the CCA cases, LT-alpha +252A/G and TNF-alpha -308G/A heterozygous and homozygous 
      variants showed significantly higher levels of these cytokines than the wild 
      type. By contrast, levels of cytokines in wild type of IFN-gamma +874T/A were 
      significantly higher than the variants in CCA cases. IFN-gamma +874T/A polymorphisms 
      were associated with advanced periductal fibrosis, whereas IL-6 -174G/C 
      polymorphisms were associated with CCA. To our knowledge, these findings provide 
      the first demonstration that O. viverrini infected individuals carrying several 
      specific cytokine gene polymorphisms are susceptible to develop fibrosis and CCA.
FAU - Surapaitoon, Arpa
AU  - Surapaitoon A
AD  - Biomedical Sciences, Graduate School, Khon Kaen University, Khon Kaen, Thailand.
AD  - WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast 
      Asian Liver Fluke Disease), Tropical Disease Research Center, Khon Kaen, 
      Thailand.
FAU - Suttiprapa, Sutas
AU  - Suttiprapa S
AD  - WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast 
      Asian Liver Fluke Disease), Tropical Disease Research Center, Khon Kaen, 
      Thailand.
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Mairiang, Eimorn
AU  - Mairiang E
AD  - Department of Radiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Khuntikeo, Narong
AU  - Khuntikeo N
AD  - Liver Fluke and Cholangiocarcinoma Research Center, and Department of Surgery, 
      Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Pairojkul, Chawalit
AU  - Pairojkul C
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
FAU - Bethony, Jeffrey
AU  - Bethony J
AD  - Department of Microbiology, Immunology and Tropical Medicine, and Research Center 
      for Neglected Tropical Diseases of Poverty, School of Medicine & Health Sciences, 
      The George Washington University, Washington DC, 20037, USA.
FAU - Brindley, Paul J
AU  - Brindley PJ
AD  - Department of Microbiology, Immunology and Tropical Medicine, and Research Center 
      for Neglected Tropical Diseases of Poverty, School of Medicine & Health Sciences, 
      The George Washington University, Washington DC, 20037, USA.
FAU - Sripa, Banchob
AU  - Sripa B
AD  - WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast 
      Asian Liver Fluke Disease), Tropical Disease Research Center, Khon Kaen, 
      Thailand.
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 
      Thailand.
LA  - eng
GR  - P50 AI098639/AI/NIAID NIH HHS/United States
GR  - R01 CA164719/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20170630
PL  - Korea (South)
TA  - Korean J Parasitol
JT  - The Korean journal of parasitology
JID - 9435800
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Bile Duct Neoplasms/*etiology/genetics/*parasitology/pathology
MH  - Bile Ducts/*pathology
MH  - Cholangiocarcinoma/*etiology/genetics/*parasitology/pathology
MH  - Cytokines/*genetics
MH  - Female
MH  - Fibrosis
MH  - *Genetic Association Studies
MH  - *Genetic Predisposition to Disease
MH  - Humans
MH  - *Inflammation Mediators
MH  - Male
MH  - Middle Aged
MH  - Opisthorchiasis/*complications/*parasitology
MH  - *Opisthorchis
MH  - Polymorphism, Genetic/*genetics
MH  - Risk
MH  - Severity of Illness Index
MH  - Young Adult
PMC - PMC5523895
OTO - NOTNLM
OT  - Opisthorchis viverrini
OT  - advanced periductal fibrosis
OT  - cholangiocarcinoma
OT  - cytokine gene polymorphisms
OT  - hepatobiliary disease
COIS- CONFLICT OF INTEREST We have no conflict of interest related to this study.
EDAT- 2017/07/20 06:00
MHDA- 2017/11/29 06:00
PMCR- 2017/06/01
CRDT- 2017/07/20 06:00
PHST- 2017/04/18 00:00 [received]
PHST- 2017/05/08 00:00 [revised]
PHST- 2017/05/11 00:00 [accepted]
PHST- 2017/07/20 06:00 [entrez]
PHST- 2017/07/20 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/06/01 00:00 [pmc-release]
AID - kjp-55-3-295 [pii]
AID - 10.3347/kjp.2017.55.3.295 [doi]
PST - ppublish
SO  - Korean J Parasitol. 2017 Jun;55(3):295-304. doi: 10.3347/kjp.2017.55.3.295. Epub 
      2017 Jun 30.