PMID- 28722422
OWN - NLM
STAT- MEDLINE
DCOM- 20180515
LR  - 20181118
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 16
IP  - 9
DP  - 2017 Sep 1
TI  - First Time View on Human Metabolome Changes after a Single Intake of 
      3,4-Methylenedioxymethamphetamine in Healthy Placebo-Controlled Subjects.
PG  - 3310-3320
LID - 10.1021/acs.jproteome.7b00294 [doi]
AB  - 3,4-Methylenedioxymethamphetamine (MDMA; "ecstasy") is widely consumed 
      recreationally. Little is known about its effects on the human metabolome. 
      Mapping biochemical changes after drug exposure can complement traditional 
      approaches by revealing potential biomarkers of organ toxicity or discovering new 
      metabolomic features in a time- and dose-dependent manner. We aimed to analyze 
      for the first time plasma samples from a randomized, double-blind, 
      placebo-controlled crossover study in healthy adults to explore changes in 
      endogenous plasma metabolites following a single intake of MDMA. Plasma samples 
      from 15 subjects taken at four different time points were analyzed with the 
      commercially available AbsoluteIDQ kit (Biocrates). Time series analysis revealed 
      a total of nine metabolites, which showed a significant concentration change 
      after MDMA administration compared with placebo. Paired t tests of the single 
      time points showed statistically significant concentration changes mainly of 
      glycerophospholipids and the metabolic ratio of methionine-sulfoxide over 
      methionine. Changes of this metabolic ratio may be indicative for changes in 
      systemic oxidative stress levels, and the increased amount of 
      glycerophospholipids could be interpreted as an upregulation of energy 
      production. Baseline samples within the experimental study design were crucial 
      for evaluation of metabolomics data as interday individuality within subjects was 
      high otherwise resulting in overestimations of the findings.
FAU - Boxler, Martina I
AU  - Boxler MI
AD  - Department of Forensic Pharmacology & Toxicology, Zurich Institute of Forensic 
      Medicine, University of Zurich ,Winterthurerstrasse 190/52, 8057 Zurich, 
      Switzerland.
FAU - Liechti, Matthias E
AU  - Liechti ME
AD  - Psychopharmacology Research, Division of Clinical Pharmacology and Toxicology, 
      Department of Biomedicine, Department of Clinical Research, University Hospital 
      Basel , 4031 Basel, Switzerland.
FAU - Schmid, Yasmin
AU  - Schmid Y
AD  - Psychopharmacology Research, Division of Clinical Pharmacology and Toxicology, 
      Department of Biomedicine, Department of Clinical Research, University Hospital 
      Basel , 4031 Basel, Switzerland.
FAU - Kraemer, Thomas
AU  - Kraemer T
AD  - Department of Forensic Pharmacology & Toxicology, Zurich Institute of Forensic 
      Medicine, University of Zurich ,Winterthurerstrasse 190/52, 8057 Zurich, 
      Switzerland.
FAU - Steuer, Andrea E
AU  - Steuer AE
AUID- ORCID: 0000-0001-8983-2353
AD  - Department of Forensic Pharmacology & Toxicology, Zurich Institute of Forensic 
      Medicine, University of Zurich ,Winterthurerstrasse 190/52, 8057 Zurich, 
      Switzerland.
LA  - eng
SI  - ClinicalTrials.gov/NCT01771874
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20170809
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Glycerophospholipids)
RN  - 0 (Hallucinogens)
RN  - 0 (Placebos)
RN  - AE28F7PNPL (Methionine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - XN1XVI4B2C (methionine sulfoxide)
SB  - IM
MH  - Adult
MH  - Chromatography, High Pressure Liquid/methods
MH  - Cross-Over Studies
MH  - Double-Blind Method
MH  - Female
MH  - Glycerophospholipids/blood
MH  - Hallucinogens/administration & dosage/*blood
MH  - Healthy Volunteers
MH  - Humans
MH  - Male
MH  - *Metabolome
MH  - Methionine/analogs & derivatives/blood
MH  - N-Methyl-3,4-methylenedioxyamphetamine/administration & dosage/*blood
MH  - *Oxidative Stress
MH  - Placebos
OTO - NOTNLM
OT  - 3,4-methylenedioxymethamphetamine (MDMA)
OT  - biocrates
OT  - ecstasy
OT  - methionine
OT  - placebo-controlled
OT  - targeted metabolomics
EDAT- 2017/07/20 06:00
MHDA- 2018/05/16 06:00
CRDT- 2017/07/20 06:00
PHST- 2017/07/20 06:00 [pubmed]
PHST- 2018/05/16 06:00 [medline]
PHST- 2017/07/20 06:00 [entrez]
AID - 10.1021/acs.jproteome.7b00294 [doi]
PST - ppublish
SO  - J Proteome Res. 2017 Sep 1;16(9):3310-3320. doi: 10.1021/acs.jproteome.7b00294. 
      Epub 2017 Aug 9.