PMID- 28724635
OWN - NLM
STAT- MEDLINE
DCOM- 20170929
LR  - 20240214
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 292
IP  - 36
DP  - 2017 Sep 8
TI  - Heterodimers of the transcriptional factors NFATc3 and FosB mediate tissue factor 
      expression for 15(S)-hydroxyeicosatetraenoic acid-induced monocyte trafficking.
PG  - 14885-14901
LID - 10.1074/jbc.M117.804344 [doi]
AB  - Tissue factor (TF) is expressed in vascular and nonvascular tissues and functions 
      in several pathways, including embryonic development, inflammation, and cell 
      migration. Many risk factors for atherosclerosis, including hypertension, 
      diabetes, obesity, and smoking, increase TF expression. To better understand the 
      TF-related mechanisms in atherosclerosis, here we investigated the role of 
      12/15-lipoxygenase (12/15-LOX) in TF expression. 15(S)-hydroxyeicosatetraenoic 
      acid (15(S)-HETE), the major product of human 15-LOXs 1 and 2, induced TF 
      expression and activity in a time-dependent manner in the human monocytic cell 
      line THP1. Moreover, TF suppression with neutralizing antibodies blocked 
      15(S)-HETE-induced monocyte migration. We also found that NADPH- and xanthine 
      oxidase-dependent reactive oxygen species (ROS) production, 
      calcium/calmodulin-dependent protein kinase IV (CaMKIV) activation, and 
      interactions between nuclear factor of activated T cells 3 (NFATc3) and FosB 
      proto-oncogene, AP-1 transcription factor subunit (FosB) are involved in 
      15(S)-HETE-induced TF expression. Interestingly, NFATc3 first induced the 
      expression of its interaction partner FosB before forming the heterodimeric 
      NFATc3-FosB transcription factor complex, which bound the proximal AP-1 site in 
      the TF gene promoter and activated TF expression. We also observed that 
      macrophages from 12/15-LOX(-/-) mice exhibit diminished migratory response to 
      monocyte chemotactic protein 1 (MCP-1) and lipopolysaccharide compared with WT 
      mouse macrophages. Similarly, compared with WT macrophages, monocytes from 
      12/15-LOX(-/-) mice displayed diminished trafficking, which was rescued by prior 
      treatment with 12(S)-HETE, in a peritonitis model. These observations indicate 
      that 15(S)-HETE-induced monocyte/macrophage migration and trafficking require 
      ROS-mediated CaMKIV activation leading to formation of NFATc3 and FosB 
      heterodimer, which binds and activates the TF promoter.
CI  - (c) 2017 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Kotla, Sivareddy
AU  - Kotla S
AD  - From the Department of Physiology, University of Tennessee Health Science Center, 
      Memphis, Tennessee 38163 and.
FAU - Singh, Nikhlesh K
AU  - Singh NK
AD  - From the Department of Physiology, University of Tennessee Health Science Center, 
      Memphis, Tennessee 38163 and.
FAU - Kirchhofer, Daniel
AU  - Kirchhofer D
AD  - Department of Early Discovery Biochemistry, Genentech Inc., South San Francisco, 
      California 94080.
FAU - Rao, Gadiparthi N
AU  - Rao GN
AD  - From the Department of Physiology, University of Tennessee Health Science Center, 
      Memphis, Tennessee 38163 and.
LA  - eng
GR  - R01 HL064165/HL/NHLBI NIH HHS/United States
GR  - R01 HL103575/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20170719
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (12-15-lipoxygenase)
RN  - 0 (FOSB protein, human)
RN  - 0 (Hydroxyeicosatetraenoic Acids)
RN  - 0 (MAS1 protein, human)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (NFATC3 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Reactive Oxygen Species)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 1.13.11.31 (Arachidonate 12-Lipoxygenase)
RN  - EC 1.13.11.33 (Arachidonate 15-Lipoxygenase)
SB  - IM
EIN - J Biol Chem. 2022 Apr;298(4):101812. PMID: 35303608
MH  - Animals
MH  - Arachidonate 12-Lipoxygenase/deficiency/genetics/*metabolism
MH  - Arachidonate 15-Lipoxygenase/deficiency/genetics/*metabolism
MH  - Cell Movement/*drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Hydroxyeicosatetraenoic Acids/*pharmacology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Monocytes/cytology/*drug effects/metabolism
MH  - NFATC Transcription Factors/*metabolism
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins c-fos/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Thromboplastin/*genetics/metabolism
MH  - Time Factors
PMC - PMC5592668
OTO - NOTNLM
OT  - gene expression
OT  - migration
OT  - monocyte
OT  - signal transduction
OT  - trafficking
COIS- The authors declare that they have no conflicts of interest with the contents of 
      this article
EDAT- 2017/07/21 06:00
MHDA- 2017/09/30 06:00
PMCR- 2018/09/08
CRDT- 2017/07/21 06:00
PHST- 2017/06/26 00:00 [received]
PHST- 2017/07/14 00:00 [revised]
PHST- 2017/07/21 06:00 [pubmed]
PHST- 2017/09/30 06:00 [medline]
PHST- 2017/07/21 06:00 [entrez]
PHST- 2018/09/08 00:00 [pmc-release]
AID - S0021-9258(20)34312-X [pii]
AID - M117.804344 [pii]
AID - 10.1074/jbc.M117.804344 [doi]
PST - ppublish
SO  - J Biol Chem. 2017 Sep 8;292(36):14885-14901. doi: 10.1074/jbc.M117.804344. Epub 
      2017 Jul 19.