PMID- 28725105
OWN - NLM
STAT- MEDLINE
DCOM- 20171227
LR  - 20240326
IS  - 1928-9022 (Electronic)
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 81
IP  - 3
DP  - 2017 Jul
TI  - Porcine reproductive and respiratory syndrome virus (PRRSV) in pig meat.
PG  - 162-170
AB  - Porcine reproductive and respiratory syndrome, caused by the porcine reproductive 
      and respiratory syndrome virus (PRRSV), is an economically important disease in 
      the swine industry. Previous studies demonstrated the presence of the virus in 
      pig meat and its transmissibility by oral consumption. This study further 
      analyzed the infectivity of PRRSV in commercial pig meat. Fresh bottom meat 
      pieces (n = 1500) randomly selected over a period of 2 y from a pork ham boning 
      plant located in Quebec, Canada, were tested by reverse transcriptase polymerase 
      chain reaction (RT-PCR). Each trimmed meat was stored in the plant freezer, 
      subsampled weekly for up to 15 wk, and tested with quantitative RT-PCR to 
      determine the viral load. Meat infectivity was evaluated using specific 
      pathogen-free piglets, each fed with approximately 500 g of meat at the end of 
      the storage time. Genotype-specific RT-PCR confirmed the presence of PRRSV mainly 
      during cold weather in 0.73% of the fresh meat pieces. Wild and vaccine strains 
      of genotype 2 were detected. Porcine reproductive and respiratory syndrome virus 
      nucleic acid was stable in meat stored at around -20 degrees C during the 15 wk. 
      Serological and molecular analysis showed the transmission of infection by a 
      majority of PRRSV positive meat pieces (5/9) fed orally to naive recipients. The 
      results confirmed a low prevalence of PRRSV in market's pig meat, and virus 
      transmissibility by oral consumption to naive recipients even after several weeks 
      of storage in a commercial freezer. It occurred mainly with meat harboring the 
      highest PRRSV RNA copies, in the range of 10(9) copies per 500 g of meat, with 
      both wild type and vaccine-related strains.
FAU - Raymond, Philippe
AU  - Raymond P
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
FAU - Bellehumeur, Christian
AU  - Bellehumeur C
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
FAU - Nagarajan, Malliga
AU  - Nagarajan M
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
FAU - Longtin, Diane
AU  - Longtin D
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
FAU - Ferland, Alexandra
AU  - Ferland A
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
FAU - Muller, Peter
AU  - Muller P
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
FAU - Bissonnette, Rachel
AU  - Bissonnette R
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
FAU - Simard, Carole
AU  - Simard C
AD  - Canadian Food Inspection Agency, Saint-Hyacinthe Laboratory, Saint-Hyacinthe, 
      Quebec J2S 8E3.
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Circovirus/isolation & purification
MH  - Food Microbiology
MH  - Genotype
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/transmission/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - RNA, Viral/genetics
MH  - Red Meat/*virology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seasons
MH  - Specific Pathogen-Free Organisms
MH  - Swine
MH  - Time Factors
MH  - Viral Load
PMC - PMC5508380
EDAT- 2017/07/21 06:00
MHDA- 2017/12/28 06:00
PMCR- 2018/01/01
CRDT- 2017/07/21 06:00
PHST- 2016/07/07 00:00 [received]
PHST- 2017/01/17 00:00 [accepted]
PHST- 2017/07/21 06:00 [entrez]
PHST- 2017/07/21 06:00 [pubmed]
PHST- 2017/12/28 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - cjvr_03_162 [pii]
PST - ppublish
SO  - Can J Vet Res. 2017 Jul;81(3):162-170.