PMID- 28727780
OWN - NLM
STAT- MEDLINE
DCOM- 20171010
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 7
DP  - 2017
TI  - Comparative analysis of signature genes in PRRSV-infected porcine 
      monocyte-derived cells to different stimuli.
PG  - e0181256
LID - 10.1371/journal.pone.0181256 [doi]
LID - e0181256
AB  - Monocyte-derived DCs (mDCs) are major target cells in porcine reproductive and 
      respiratory syndrome virus (PRRSV) pathogenesis; however, the plasticity of mDCs 
      in response to activation stimuli and PRRSV infection remains unstudied. In this 
      study, we polarized mDCs, and applied genome-wide transcriptomic analysis and 
      predicted protein-protein interaction networks to compare signature genes 
      involved in mDCs activation and response to PRRSV infection. Porcine mDCs were 
      polarized with mediators for 30 hours, then mock-infected, infected with PRRSV 
      strain VR2332, or a highly pathogenic PRRSV strain (rJXwn06), for 5 h. Total RNA 
      was extracted and used to construct sequencing libraries for RNA-Seq. Comparisons 
      were made between each polarized and unpolarized group (i.e. mediator vs. PBS), 
      and between PRRSV-infected and uninfected cells stimulated with the same 
      mediator. Differentially expressed genes (DEG) from the comparisons were used for 
      prediction of interaction networks affected by the viruses and mediators. The 
      results showed that PRRSV infection inhibited M1-prone immune activity, 
      downregulated genes, predicted network interactions related to cellular 
      integrity, and inflammatory signaling in favor of M2 activity. Additionally, the 
      number of DEG and predicted network interactions stimulated in HP-PRRSV infected 
      mDCs was superior to the VR-2332 infected mDCs and conformed with HP-PRRSV 
      pathogenicity.
FAU - Miller, Laura C
AU  - Miller LC
AUID- ORCID: 0000-0002-8946-9416
AD  - Virus and Prion Diseases of Livestock Research Unit, National Animal Disease 
      Center, USDA-ARS, 1920 Dayton Ave, Ames, United States of America.
FAU - Fleming, Damarius S
AU  - Fleming DS
AD  - Virus and Prion Diseases of Livestock Research Unit, National Animal Disease 
      Center, USDA-ARS, 1920 Dayton Ave, Ames, United States of America.
FAU - Li, Xiangdong
AU  - Li X
AD  - National Research Center for Veterinary Medicine, Cuiwei Rd, High-Tech District, 
      Luoyang, Henan, China.
FAU - Bayles, Darrell O
AU  - Bayles DO
AD  - Infectious Bacterial Diseases Research Unit, National Animal Disease Center, 
      USDA-ARS, 1920 Dayton Ave, Ames, United States of America.
FAU - Blecha, Frank
AU  - Blecha F
AD  - Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas 
      State University, Manhattan, United States of America.
FAU - Sang, Yongming
AU  - Sang Y
AD  - Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas 
      State University, Manhattan, United States of America.
AD  - Department of Agricultural and Environmental Sciences, College of Agriculture, 
      Human and Natural Sciences, Tennessee State University, 3500 John A. Merritt 
      Boulevard, Nashville, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20170720
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - Cell Polarity
MH  - Gene Expression Profiling
MH  - Gene Regulatory Networks
MH  - Monocytes/cytology/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics
MH  - Protein Interaction Maps
MH  - Signal Transduction
MH  - Swine
MH  - Transcriptome
PMC - PMC5519058
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/07/21 06:00
MHDA- 2017/10/11 06:00
PMCR- 2017/07/20
CRDT- 2017/07/21 06:00
PHST- 2016/10/27 00:00 [received]
PHST- 2017/06/28 00:00 [accepted]
PHST- 2017/07/21 06:00 [entrez]
PHST- 2017/07/21 06:00 [pubmed]
PHST- 2017/10/11 06:00 [medline]
PHST- 2017/07/20 00:00 [pmc-release]
AID - PONE-D-16-42832 [pii]
AID - 10.1371/journal.pone.0181256 [doi]
PST - epublish
SO  - PLoS One. 2017 Jul 20;12(7):e0181256. doi: 10.1371/journal.pone.0181256. 
      eCollection 2017.