PMID- 28728363
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1011-2367 (Print)
IS  - 1976-5517 (Electronic)
IS  - 1011-2367 (Linking)
VI  - 31
IP  - 2
DP  - 2018 Feb
TI  - Effect of different levels of protein concentrates supplementation on the growth 
      performance, plasma amino acids profile and mTOR cascade genes expression in 
      early-weaned yak calves.
PG  - 218-224
LID - 10.5713/ajas.16.0999 [doi]
AB  - OBJECTIVE: This study evaluated the effects of different levels of protein 
      concentrate supplementation on the growth performance of yak calves, and 
      correlated the growth rate to changes occurring in the plasma- amino acids, 
      -insulin profile, and signaling activity of mammalian target of rapamycin (mTOR) 
      cascade to characterize the mechanism through which the protein synthesis can be 
      improved in early weaned yaks. METHODS: For this study, 48 early (3 months old) 
      weaned yak calves were selected, and assigned into four dietary treatments 
      according to randomized complete block design. The four blocks were balanced for 
      body weight and sex. The yaks were either grazed on natural pasture (control 
      diet) in a single herd or the grazing yaks was supplemented with one of the three 
      protein rich supplements containing low (17%; LP), medium (19%; MP), or high 
      (21%; HP) levels of crude proteins for a period of 30 days. RESULTS: Results 
      showed that the average daily gain of calves increased (0.14 vs 0.23-0.26 kg; 
      p<0.05) with protein concentrates supplementation. The concentration of plasma 
      methionine increased (p<0.05; 8.6 vs 10.1-12.4 mumol/L), while those of serine and 
      tyrosine did not change (p>0.05) when the grazing calves were supplemented with 
      protein concentrates. Compared to control diet, the insulin level of calves 
      increased (p<0.05; 1.86 vs 2.16-2.54 muIU/mL) with supplementation of protein 
      concentrates. Addition of protein concentrates up-regulated (p<0.05) expression 
      of mTOR-raptor, mammalian vacuolar protein sorting 34 homolog, the translational 
      regulators eukaryotic translation initiation factor 4E binding protein 1, and S6 
      kinase 1 genes in both Longissimus dorsi and semitendinosus. In contrast, the 
      expression of sequestosome 1 was down-regulated in the concentrate supplemented 
      calves. CONCLUSION: Our results show that protein supplementation improves the 
      growth performance of early weaned yak calves, and that plasma methionine and 
      insulin concentrations were the key mediator for gene expression and protein 
      deposition in the muscles.
FAU - Peng, Q H
AU  - Peng QH
AUID- ORCID: 0000-0003-1421-9145
AD  - Animal Nutrition Institute of Sichuan Agricultural University, Key Laboratory of 
      Bovine Low-Carbon Farming and Safe Production, Ya'an 625014, China.
FAU - Khan, N A
AU  - Khan NA
AUID- ORCID: 0000-0001-5969-8616
AD  - Department of Animal Nutrition, The University of Agriculture Peshawar, KP 25120, 
      Pakistan.
FAU - Xue, B
AU  - Xue B
AUID- ORCID: 0000-0001-9283-3015
AD  - Animal Nutrition Institute of Sichuan Agricultural University, Key Laboratory of 
      Bovine Low-Carbon Farming and Safe Production, Ya'an 625014, China.
FAU - Yan, T H
AU  - Yan TH
AUID- ORCID: 0000-0002-1994-5202
AD  - Animal Nutrition Institute of Sichuan Agricultural University, Key Laboratory of 
      Bovine Low-Carbon Farming and Safe Production, Ya'an 625014, China.
AD  - Agri-Food and Biosciences Institute, Hillsborough, Co Down BT26 6DR, UK.
FAU - Wang, Z S
AU  - Wang ZS
AUID- ORCID: 0000-0001-7999-3612
AD  - Animal Nutrition Institute of Sichuan Agricultural University, Key Laboratory of 
      Bovine Low-Carbon Farming and Safe Production, Ya'an 625014, China.
LA  - eng
PT  - Journal Article
DEP - 20170627
PL  - Korea (South)
TA  - Asian-Australas J Anim Sci
JT  - Asian-Australasian journal of animal sciences
JID - 9884245
PMC - PMC5767503
OTO - NOTNLM
OT  - Early Weaning
OT  - Protein Synthesis
OT  - Supplementary Feeding
OT  - Yak
OT  - Yak Calves Growth
COIS- CONFLICT OF INTEREST We certify that there is no conflict of interest with any 
      financial organization regarding the material discussed in the manuscript.
EDAT- 2017/07/22 06:00
MHDA- 2017/07/22 06:01
PMCR- 2018/02/01
CRDT- 2017/07/22 06:00
PHST- 2016/12/10 00:00 [received]
PHST- 2017/03/26 00:00 [revised]
PHST- 2017/06/13 00:00 [accepted]
PHST- 2017/07/22 06:00 [pubmed]
PHST- 2017/07/22 06:01 [medline]
PHST- 2017/07/22 06:00 [entrez]
PHST- 2018/02/01 00:00 [pmc-release]
AID - ajas.16.0999 [pii]
AID - ajas-31-2-218 [pii]
AID - 10.5713/ajas.16.0999 [doi]
PST - ppublish
SO  - Asian-Australas J Anim Sci. 2018 Feb;31(2):218-224. doi: 10.5713/ajas.16.0999. 
      Epub 2017 Jun 27.