PMID- 28728385 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20240327 IS - 1011-2367 (Print) IS - 1976-5517 (Electronic) IS - 1011-2367 (Linking) VI - 30 IP - 12 DP - 2017 Dec TI - Effects of dietary energy sources on early postmortem muscle metabolism of finishing pigs. PG - 1764-1772 LID - 10.5713/ajas.17.0090 [doi] AB - OBJECTIVE: This study investigated the effects of different dietary energy sources on early postmortem muscle metabolism of finishing pigs. METHODS: Seventy-two barrow (DurocxLandracexYorkshire, DLY) pigs (65.0+/-2.0 kg) were allotted to three iso-energetic and iso-nitrogenous diets: A (44.1% starch, 5.9% crude fat, and 12.6% neutral detergent fibre [NDF]), B (37.6% starch, 9.5% crude fat, and 15.4% NDF) or C (30.9% starch, 14.3% crude fat, and 17.8% NDF). After the duration of 28-day feeding experiment, 24 pigs (eight per treatment) were slaughtered and the M. longissimus lumborum (LL) samples at 45 min postmortem were collected. RESULTS: Compared with diet A, diet C resulted in greater adenosine triphosphate and decreased phosphocreatine (PCr) concentrations, greater activity of creatine kinase and reduced percentage bound activities of hexokinase (HK), and pyruvate kinase (PK) in LL muscles (p<0.05). Moreover, diet C decreased the phosphor-AKT level and increased the hydroxy-hypoxia-inducible factor-1alpha (HIF-1alpha) level, as well as decreased the bound protein expressions of HK II, PKM2, and lactate dehydrogenase A (p<0.05). CONCLUSION: Diet C with the lowest level of starch and the highest levels of fat and NDF could enhance the PCr utilization and attenuate glycolysis early postmortem in LL muscle of finishing pigs. FAU - Li, Yanjiao AU - Li Y AD - College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. AD - College of Animal Science and Technology, Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, China. FAU - Yu, Changning AU - Yu C AD - College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. FAU - Li, Jiaolong AU - Li J AD - College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. FAU - Zhang, Lin AU - Zhang L AD - College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. FAU - Gao, Feng AU - Gao F AD - College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. FAU - Zhou, Guanghong AU - Zhou G AD - College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China. LA - eng PT - Journal Article DEP - 20170626 PL - Korea (South) TA - Asian-Australas J Anim Sci JT - Asian-Australasian journal of animal sciences JID - 9884245 PMC - PMC5666181 OTO - NOTNLM OT - Dietary Energy Source OT - Finishing Pigs OT - Glycolytic Enzyme OT - PI3K/AKT-HIF-1alpha Pathway OT - Postmortem Metabolism COIS- CONFLICT OF INTEREST We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript. EDAT- 2017/07/22 06:00 MHDA- 2017/07/22 06:01 PMCR- 2017/12/01 CRDT- 2017/07/22 06:00 PHST- 2017/02/11 00:00 [received] PHST- 2017/04/26 00:00 [revised] PHST- 2017/05/22 00:00 [accepted] PHST- 2017/07/22 06:00 [pubmed] PHST- 2017/07/22 06:01 [medline] PHST- 2017/07/22 06:00 [entrez] PHST- 2017/12/01 00:00 [pmc-release] AID - ajas.17.0090 [pii] AID - ajas-30-12-1764 [pii] AID - 10.5713/ajas.17.0090 [doi] PST - ppublish SO - Asian-Australas J Anim Sci. 2017 Dec;30(12):1764-1772. doi: 10.5713/ajas.17.0090. Epub 2017 Jun 26.