PMID- 2872917
OWN - NLM
STAT- MEDLINE
DCOM- 19860801
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 25
IP  - 10
DP  - 1986 May 20
TI  - Structural aspects and orientation mechanism of mitochondrial F1 
      adenosinetriphosphatase. Evidence for a negative electric birefringence due to a 
      permanent moment perpendicular to the long axes of the particle.
PG  - 2854-8
AB  - The electric birefringence technique was used to investigate the steady-state 
      birefringence, the orientational relaxation time, and the orientation mechanism 
      of pig heart mitochondrial F1 adenosine-5'-triphosphatase (F1-ATPase). The 
      electrooptical properties of this enzyme in solution were studied as functions of 
      pH, protein concentration, and applied electric field. The F1-ATPase exhibits a 
      surprising negative electric birefringence with a specific Kerr constant of -1.5 
      X 10(-3) esu cgs. The field-independent relaxation time was found to be 0.65 +/- 
      0.05 microseconds, corresponding to a rotational diffusion constant of 2.55 X 
      10(5) s-1. The overall size and shape of F1-ATPase have been calculated from both 
      translational and rotational diffusion constants. The enzyme may be assumed to be 
      an oblate ellipsoid of revolution with dimensions of about 170 X 170 X 70 A. The 
      orientation mechanism of F1-ATPase was analyzed by fitting experimental 
      birefringence rising curves with theoretical rising functions. The ratio of the 
      permanent to induced dipole moment is found to be very high; therefore, the 
      birefringence of F1-ATPase is due to a strong permanent dipole moment in a 
      direction perpendicular to the long axes of the particle. These particular 
      electric properties can be explained by the oligomeric structure of the protein 
      and seem likely to play a role in its mechanism of functioning.
FAU - Deleage, G
AU  - Deleage G
FAU - Roux, B
AU  - Roux B
FAU - Marion, C
AU  - Marion C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - EC 3.6.3.14 (Proton-Translocating ATPases)
SB  - IM
MH  - Animals
MH  - Birefringence
MH  - Kinetics
MH  - Mathematics
MH  - Mitochondria, Heart/*enzymology
MH  - Protein Conformation
MH  - Proton-Translocating ATPases/*metabolism
MH  - Swine
EDAT- 1986/05/20 00:00
MHDA- 1986/05/20 00:01
CRDT- 1986/05/20 00:00
PHST- 1986/05/20 00:00 [pubmed]
PHST- 1986/05/20 00:01 [medline]
PHST- 1986/05/20 00:00 [entrez]
AID - 10.1021/bi00358a017 [doi]
PST - ppublish
SO  - Biochemistry. 1986 May 20;25(10):2854-8. doi: 10.1021/bi00358a017.