PMID- 28730101
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 10
IP  - 7
DP  - 2017
TI  - Comparative analysis of different feeder layers with 3T3 fibroblasts for 
      culturing rabbits limbal stem cells.
PG  - 1021-1027
LID - 10.18240/ijo.2017.07.01 [doi]
AB  - AIM: To explore the possibility of human umbilical cord mesenchymal stem cells 
      (hUCMSCs), human umbilical vein endothelial cells (hUVECs), human dental pulp 
      stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) serving 
      as feeder cells in co-culture systems for the cultivation of limbal stem cells. 
      METHODS: Different feeder layers were cultured in Dulbecco's modified Eagle's 
      medium (DMEM)/F12 and were treated with mitomycin C. Rabbits limbal stem cells 
      (LSCs) were co-cultured on hUCMSCs, hUVECs, hDPSCs, hPDLSCs and NIH-3T3, and then 
      comparative analysis were made between each group to see their respective 
      colony-forming efficiency (CFE) assay and immunofluorescence (IPO13,CK3/12). 
      RESULTS: The efficiency of the four type cells in supporting the LSCs morphology 
      and its cellular differentiation was similar to that of NIH-3T3 fibroblasts as 
      demonstrated by the immunostaining properties analysis, with each group 
      exhibiting a similar strong expression pattern of IPO13, but lacking CK3 and CK12 
      expression in terms of immunostaining. But hUCMSCs, hDPSCs and hPDLSCs feeder 
      layers were superior in promoting colony formation potential of cells when 
      compared to hUVECs and feeder-cell-free culture. CONCLUSION: hUCMSCs, hDPSCs and 
      hPDLSCs can be a suitable alternative to conventional mouse NIH-3T3 feeder cells, 
      so that risk of zoonotic infection can be diminished.
FAU - Wang, Hui-Xian
AU  - Wang HX
AD  - Medical College of Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous 
      Region, China.
AD  - Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur 
      Autonomous Region, China.
FAU - Gao, Xiao-Wei
AU  - Gao XW
AD  - Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur 
      Autonomous Region, China.
FAU - Ren, Bing
AU  - Ren B
AD  - Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur 
      Autonomous Region, China.
FAU - Cai, Yan
AU  - Cai Y
AD  - Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur 
      Autonomous Region, China.
FAU - Li, Wen-Jing
AU  - Li WJ
AD  - Ophthalmic Center, No.474 Hospital of Chinese PLA, Urumqi 830013, Xinjiang Uygur 
      Autonomous Region, China.
FAU - Yang, Yu-Li
AU  - Yang YL
AD  - Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
FAU - Li, Yi-Jian
AU  - Li YJ
AD  - Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
LA  - eng
PT  - Journal Article
DEP - 20170718
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC5514260
OTO - NOTNLM
OT  - dental pulp stem cells
OT  - feeder layers
OT  - limbal stem cells
OT  - periodontal ligament stem cells
OT  - umbilical cord mesenchymal stem cells
OT  - umbilical vein endothelial cells
EDAT- 2017/07/22 06:00
MHDA- 2017/07/22 06:01
PMCR- 2017/07/18
CRDT- 2017/07/22 06:00
PHST- 2016/08/10 00:00 [received]
PHST- 2017/03/31 00:00 [accepted]
PHST- 2017/07/22 06:00 [entrez]
PHST- 2017/07/22 06:00 [pubmed]
PHST- 2017/07/22 06:01 [medline]
PHST- 2017/07/18 00:00 [pmc-release]
AID - ijo-10-07-1021 [pii]
AID - 10.18240/ijo.2017.07.01 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2017 Jul 18;10(7):1021-1027. doi: 10.18240/ijo.2017.07.01. 
      eCollection 2017.