PMID- 28730645
OWN - NLM
STAT- MEDLINE
DCOM- 20180918
LR  - 20180918
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
VI  - 50
IP  - 2
DP  - 2018 Mar
TI  - Effect of metformin on germ cell-specific apoptosis, oxidative stress and 
      epididymal sperm quality after testicular torsion/detorsion in rats.
LID - 10.1111/and.12846 [doi]
AB  - The study was designed to evaluate the effects of metformin on apoptosis and 
      epididymal sperm quality in a rat testicular ischaemia/reperfusion (I/R) injury 
      model. A total of 72 male rats were divided into four groups (n = 18 for each 
      group): group 1 (sham-operated group), group 2 (metformin group), group 3 
      (torsion/detorsion [T/D] + saline) and group 4 (T/D + 300 mg kg(-1) metformin). 
      Testicular torsion was achieved by rotating the right testis 720 degrees  in a clockwise 
      direction for 1 hr. Tissue malondialdehyde (MDA) level and caspase-3 activity 
      increased and the activities of catalase, superoxide dismutase and glutathione 
      peroxidase decreased in comparison with sham-operated group 4 hr after detorsion 
      (p < .001). In six rats of each group 24 hr after detorsion, histopathological 
      changes and germ cell apoptosis were significantly deteriorated by measuring mean 
      of seminiferous tubule diameters (MSTD) and TUNEL test. Moreover, 30 days after 
      T/D, sperm concentration and motility were examined in six animals per group. 
      Metformin pre-treatment reduced MDA and caspase-3 levels and normalised 
      antioxidant enzyme activities 4 hr after detorsion, and germ cell apoptosis was 
      significantly decreased, and the MSTD, as well as sperm functions, was 
      significantly improved. Reduction in oxidative stress and apoptosis may have a 
      major role in cytoprotective effects of metformin.
CI  - (c) 2017 Blackwell Verlag GmbH.
FAU - Ghasemnejad-Berenji, M
AU  - Ghasemnejad-Berenji M
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
AD  - Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical 
      Sciences, Urmia, Iran.
FAU - Ghazi-Khansari, M
AU  - Ghazi-Khansari M
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Yazdani, I
AU  - Yazdani I
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Nobakht, M
AU  - Nobakht M
AD  - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, 
      Tehran, Iran.
FAU - Abdollahi, A
AU  - Abdollahi A
AD  - Department of Pathology, Cancer Institute, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Ghasemnejad-Berenji, H
AU  - Ghasemnejad-Berenji H
AD  - Department of Anatomy and Reproductive Biology, School of Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Mohajer Ansari, J
AU  - Mohajer Ansari J
AD  - Department of Anatomy, School of Medicine, Iran University of Medical Sciences, 
      Tehran, Iran.
FAU - Pashapour, S
AU  - Pashapour S
AD  - Department of Pathology, Urmia University of Medical Science, Urmia, Iran.
FAU - Dehpour, A R
AU  - Dehpour AR
AUID- ORCID: 0000-0002-8001-5565
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20170721
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
RN  - 0 (Enzyme Activators)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 9100L32L2N (Metformin)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Caspase 3/metabolism
MH  - Catalase/metabolism
MH  - Disease Models, Animal
MH  - Enzyme Activators/*pharmacology/therapeutic use
MH  - Epididymis/cytology/drug effects/pathology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Metformin/*pharmacology/therapeutic use
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/drug therapy/etiology/*physiopathology
MH  - Sperm Count
MH  - Spermatic Cord Torsion/complications
MH  - Spermatozoa/drug effects/*physiology
MH  - Testis/pathology
OTO - NOTNLM
OT  - apoptosis
OT  - metformin
OT  - oxidative stress
OT  - testicular torsion/detorsion
EDAT- 2017/07/22 06:00
MHDA- 2018/09/19 06:00
CRDT- 2017/07/22 06:00
PHST- 2017/03/21 00:00 [accepted]
PHST- 2017/07/22 06:00 [pubmed]
PHST- 2018/09/19 06:00 [medline]
PHST- 2017/07/22 06:00 [entrez]
AID - 10.1111/and.12846 [doi]
PST - ppublish
SO  - Andrologia. 2018 Mar;50(2). doi: 10.1111/and.12846. Epub 2017 Jul 21.