PMID- 28730835
OWN - NLM
STAT- MEDLINE
DCOM- 20180718
LR  - 20200116
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 66
IP  - 5
DP  - 2017 Nov 24
TI  - The effect of very-low-calorie diet on mitochondrial dysfunction in subcutaneous 
      adipose tissue and peripheral monocytes of obese subjects with type 2 diabetes 
      mellitus.
PG  - 811-822
AB  - Mitochondrial dysfunction is a potentially important player in the development of 
      insulin resistance and type 2 diabetes mellitus (T2DM). We investigated the 
      changes of mRNA expression of genes encoding main enzymatic complexes of 
      mitochondrial respiratory chain in subcutaneous adipose tissue (SCAT) and 
      peripheral monocytes (PM) of 11 subjects with simple obesity (OB), 16 obese 
      patients with T2DM and 17 healthy lean subjects (C) before and after very 
      low-calorie diet (VLCD) using quantitative real time PCR. At baseline in SCAT, 
      both T2DM and OB group had decreased mRNA expression of all investigated 
      mitochondrial genes with the exception of 2 complex I (NDUFA 12) and complex IV 
      (COX 4/1) enzymes in OB subjects. In contrast, in PM only the expression of 
      complex I enzymes NDUFA 12 and MT-ND5 was reduced in both T2DM and OB subjects 
      along with decreased expression of citrate synthase (CS) in T2DM group. 
      Additionally, T2DM subjects showed reduced activity of pyruvate dehydrogenase and 
      complex IV in peripheral blood elements. VLCD further decreased mRNA expression 
      of CS and complex I (NT-ND5) and II (SDHA) enzymes in SCAT and complex IV 
      (COX4/1) and ATP synthase in PM of T2DM group, while increasing the activity of 
      complex IV in their peripheral blood elements. We conclude that impaired 
      mitochondrial biogenesis and decreased activity of respiratory chain enzymatic 
      complexes was present in SCAT and PM of obese and diabetic patients. VLCD 
      improved metabolic parameters and ameliorated mitochondrial oxidative function in 
      peripheral blood elements of T2DM subjects but had only minor and inconsistent 
      effect on mitochondrial gene mRNA expression in SCAT and PM.
FAU - Urbanova, M
AU  - Urbanova M
AD  - Institute of Rheumatology, Prague, Czech Republic, Centre for Experimental 
      Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech 
      Republic. halm@medicon.cz.
FAU - Mraz, M
AU  - Mraz M
FAU - Durovcova, V
AU  - Durovcova V
FAU - Trachta, P
AU  - Trachta P
FAU - Klouckova, J
AU  - Klouckova J
FAU - Kavalkova, P
AU  - Kavalkova P
FAU - Haluzikova, D
AU  - Haluzikova D
FAU - Lacinova, Z
AU  - Lacinova Z
FAU - Hansikova, H
AU  - Hansikova H
FAU - Wenchich, L
AU  - Wenchich L
FAU - Krsek, M
AU  - Krsek M
FAU - Haluzik, M
AU  - Haluzik M
LA  - eng
PT  - Journal Article
DEP - 20170718
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
SB  - IM
MH  - Adult
MH  - Caloric Restriction/*methods/trends
MH  - Diabetes Mellitus, Type 2/*blood/diet therapy/epidemiology
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Mitochondria/*metabolism
MH  - Obesity/*blood/diet therapy/epidemiology
MH  - Subcutaneous Fat/*metabolism
MH  - Treatment Outcome
EDAT- 2017/07/22 06:00
MHDA- 2018/07/19 06:00
CRDT- 2017/07/22 06:00
PHST- 2017/07/22 06:00 [pubmed]
PHST- 2018/07/19 06:00 [medline]
PHST- 2017/07/22 06:00 [entrez]
AID - 933469 [pii]
AID - 10.33549/physiolres.933469 [doi]
PST - ppublish
SO  - Physiol Res. 2017 Nov 24;66(5):811-822. doi: 10.33549/physiolres.933469. Epub 
      2017 Jul 18.