PMID- 28732679
OWN - NLM
STAT- MEDLINE
DCOM- 20190312
LR  - 20190312
IS  - 1879-1220 (Electronic)
IS  - 0960-0760 (Linking)
VI  - 177
DP  - 2018 Mar
TI  - Serum 25-hydroxyvitamin D concentrations are associated with nuclear factor 
      kappa-B activity but not with inflammatory markers in healthy normoglycemic 
      adults.
PG  - 216-222
LID - S0960-0760(17)30178-4 [pii]
LID - 10.1016/j.jsbmb.2017.07.013 [doi]
AB  - Vitamin D has been reported to have anti-inflammatory properties in in vitro and 
      animal studies, which are thought to occur via inhibition of the nuclear factor 
      kappa-B (NFkappaB) pathway. However, the association between vitamin D and in vivo 
      NFkappaB activity in humans has not previously been reported. The aim of the present 
      study was to examine the associations between circulating 25-hydroxyvitamin D 
      (25(OH)D) concentrations and NFkappaB activity in peripheral blood mononuclear cells 
      (PBMCs) as well as plasma inflammatory markers in healthy individuals. We 
      hypothesized that 25(OH)D concentrations would be negatively associated with NFkappaB 
      activity and pro-inflammatory markers downstream of NFkappaB, and positively 
      associated with anti-inflammatory markers. We measured circulating 25(OH)D 
      (chemiluminescent immunoassay); anthropometry: body mass index (BMI), 
      waist-to-hip ratio (WHR), and % body fat (dual X-ray absorptiometry); plasma pro- 
      and anti-inflammatory markers: high sensitivity C-reactive protein (hsCRP), tumor 
      necrosis factor (TNF), monocyte chemoattractant protein-1 (MCP-1), interleukin-6 
      (IL-6), and IL-10 (ELISA); and NFkappaB activity in PBMCs (DNA-binding assay). 
      Forty-nine participants were included in the study (21M/28F; age=31.6+/-10.2years 
      (mean+/-SD); BMI=28.4+/-4.6kg/m(2); % body fat=30.2+/-9.3%). Mean 25(OH)D concentration 
      was 48.2+/-24.5 nmol/l. There were no differences in 25(OH)D concentrations between 
      genders and no association between 25(OH)D concentrations and age, BMI, or % body 
      fat (all p>0.1). Serum 25(OH)D concentrations were positively associated with 
      NFkappaB activity in PBMCs (r=0.48, p=0.0008) but not with any of the pro- or 
      anti-inflammatory markers measured (all p>0.1). After adjusting for age, sex, and 
      % body fat, 25(OH)D concentrations remained positively associated with NFkappaB 
      activity in PBMCs (beta=0.55, p<0.0001). Although in-vitro studies suggest that 
      vitamin D inhibits NFkappaB activity, our novel cross-sectional data from a cohort of 
      healthy individuals suggest that vitamin D may regulate rather than inhibit the 
      NFkappaB pathway. Large-scale intervention and mechanistic studies are needed to 
      further investigate the effects of vitamin D on NFkappaB activity in vivo in humans.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Mousa, Aya
AU  - Mousa A
AD  - Monash Centre for Health Research and Implementation (MCHRI), School of Public 
      Health and Preventive Medicine, Monash University, 43-51 Kanooka Grove, Clayton, 
      Melbourne, VIC 3168, Australia. Electronic address: aya.mousa@monash.edu.
FAU - de Courten, Maximilian P J
AU  - de Courten MPJ
AD  - Centre for Chronic Disease, Victoria University, 176 Furlong Road, St Albans, 
      Melbourne, VIC 3021, Australia. Electronic address: 
      Maximilian.deCourten@vu.edu.au.
FAU - Forbes, Josephine
AU  - Forbes J
AD  - Mater Medical Research Institute, Level 3, Aubigny Place, Raymond Terrace, South 
      Brisbane, QLD 4101, Australia. Electronic address: 
      josephine.forbes@mater.uq.edu.au.
FAU - de Courten, Barbora
AU  - de Courten B
AD  - Monash Centre for Health Research and Implementation (MCHRI), School of Public 
      Health and Preventive Medicine, Monash University, 43-51 Kanooka Grove, Clayton, 
      Melbourne, VIC 3168, Australia. Electronic address: barbora.decourten@monash.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170718
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 1406-16-2 (Vitamin D)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - A288AR3C9H (25-hydroxyvitamin D)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - C-Reactive Protein/analysis
MH  - Cytokines/blood
MH  - Female
MH  - Humans
MH  - Inflammation/*blood
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
MH  - NF-kappa B/*blood
MH  - Vitamin D/*analogs & derivatives/blood
MH  - Young Adult
OTO - NOTNLM
OT  - 25(OH)D
OT  - Inflammation
OT  - Nuclear factor kappa-B
OT  - Vitamin D
EDAT- 2017/07/25 06:00
MHDA- 2019/03/13 06:00
CRDT- 2017/07/23 06:00
PHST- 2017/04/27 00:00 [received]
PHST- 2017/06/30 00:00 [revised]
PHST- 2017/07/15 00:00 [accepted]
PHST- 2017/07/25 06:00 [pubmed]
PHST- 2019/03/13 06:00 [medline]
PHST- 2017/07/23 06:00 [entrez]
AID - S0960-0760(17)30178-4 [pii]
AID - 10.1016/j.jsbmb.2017.07.013 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2018 Mar;177:216-222. doi: 
      10.1016/j.jsbmb.2017.07.013. Epub 2017 Jul 18.