PMID- 28733114
OWN - NLM
STAT- MEDLINE
DCOM- 20180418
LR  - 20180418
IS  - 1872-9096 (Electronic)
IS  - 0166-3542 (Linking)
VI  - 145
DP  - 2017 Sep
TI  - Targeting heat shock factor 1 as an antiviral strategy against dengue virus 
      replication in vitro and in vivo.
PG  - 44-53
LID - S0166-3542(17)30092-X [pii]
LID - 10.1016/j.antiviral.2017.07.008 [doi]
AB  - Fever onset is correlated with viremia in dengue virus (DENV) patients. Heat 
      shock factor 1 (HSF1), a heat stress response host transcription factor, plays a 
      crucial role in regulating multiple cellular functions, as well as the onset of 
      infectious diseases. This study evaluated the role of HSF1 in DENV replication as 
      a means of regulating DENV infection in vitro and in vivo. DENV infection 
      activated HSF1 in both Ca(2+) and protein kinase A-dependent manners. Inhibiting 
      HSF1 effectively reduced DENV replication, not only in THP-1 cells but also in 
      primary human monocytes. Activated HSF1 contributed to DENV replication by 
      upregulating autophagy-related protein (Atg) 7, as autophagy is crucial for virus 
      replication. Heat stress also activated HSF1, which in turn facilitated DENV 
      replication. Activated HSF1, the increased Atg7, and autophagic induction were 
      founded in the DENV-infected brains and pharmacologically inhibiting HSF1 reduced 
      autophagy, viral protein expression, neuropathy, and mortality. These results 
      provide new insight into HSF1 as a novel host factor for DENV infection through 
      its role in facilitating autophagy-regulated viral replication in the brains.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Tsai, Tsung-Ting
AU  - Tsai TT
AD  - Department of Microbiology and Immunology, School of Medicine, College of 
      Medicine, Taipei Medical University, Taipei 110, Taiwan.
FAU - Chen, Chia-Ling
AU  - Chen CL
AD  - Translational Research Center, Taipei Medical University, Taipei 110, Taiwan.
FAU - Tsai, Cheng-Chieh
AU  - Tsai CC
AD  - Department of Nursing, Chung Hwa University of Medical Technology, Tainan 717, 
      Taiwan.
FAU - Lin, Chiou-Feng
AU  - Lin CF
AD  - Department of Microbiology and Immunology, School of Medicine, College of 
      Medicine, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of 
      Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, 
      Taiwan. Electronic address: cflin2014@tmu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20170718
PL  - Netherlands
TA  - Antiviral Res
JT  - Antiviral research
JID - 8109699
RN  - 0 (Antiviral Agents)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Hsf1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*pharmacology/*therapeutic use
MH  - Autophagy
MH  - Cell Line
MH  - DNA Replication/drug effects
MH  - Dengue/drug therapy/virology
MH  - Dengue Virus/*drug effects/physiology
MH  - Heat Shock Transcription Factors/*antagonists & inhibitors/*physiology
MH  - Humans
MH  - Mice
MH  - Monocytes/drug effects/virology
MH  - Virus Replication/*drug effects
OTO - NOTNLM
OT  - Autophagy
OT  - Dengue virus
OT  - HSF1
OT  - Infection
OT  - Neuropathy
OT  - Replication
EDAT- 2017/07/25 06:00
MHDA- 2018/04/19 06:00
CRDT- 2017/07/23 06:00
PHST- 2017/02/13 00:00 [received]
PHST- 2017/07/12 00:00 [revised]
PHST- 2017/07/13 00:00 [accepted]
PHST- 2017/07/25 06:00 [pubmed]
PHST- 2018/04/19 06:00 [medline]
PHST- 2017/07/23 06:00 [entrez]
AID - S0166-3542(17)30092-X [pii]
AID - 10.1016/j.antiviral.2017.07.008 [doi]
PST - ppublish
SO  - Antiviral Res. 2017 Sep;145:44-53. doi: 10.1016/j.antiviral.2017.07.008. Epub 
      2017 Jul 18.