PMID- 28735223
OWN - NLM
STAT- MEDLINE
DCOM- 20170926
LR  - 20170926
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1061-1062
DP  - 2017 Sep 1
TI  - Isomeric separation and quantitation of di-(2-ethylhexyl) trimellitates and 
      mono-(2-ethylhexyl) trimellitates in blood by LC-MS/MS.
PG  - 153-162
LID - S1570-0232(17)30426-9 [pii]
LID - 10.1016/j.jchromb.2017.07.014 [doi]
AB  - A new and fast HPLC-method for the simultaneous determination of 
      tri-(2-ethylhexyl) trimellitate (TOTM or TEHTM), its diesters 
      2,4-di-(2-ethylhexyl) trimellitate (2,4-DEHTM), 1,4-di-(2-ethylhexyl) 
      trimellitate (1,4-DEHTM), 1,2-di-(2-ethylhexyl) trimellitate (1,2-DEHTM) and 
      monoesters 1-mono-(2-ethylhexyl) trimellitate (1-MEHTM), 2-mono-(2-ethylhexyl) 
      trimellitate (2-MEHTM) and 4-mono-(2-ethylhexyl) trimellitate (4-MEHTM) together 
      with di-(2-ethylhexyl) phthalate (DEHP) and its primary metabolite 
      mono-(2-ethylhexyl) phthalate (MEHP) in blood was developed and validated. The 
      analytes are extracted from blood using liquid-liquid extraction and are 
      chromatographically separated by reversed-phase HPLC using core shell material. 
      Quantitative assessment was performed by ESI-tandem mass spectrometry in negative 
      ionization mode using stable isotope dilution. In less than 30min six postulated 
      primary metabolites of TOTM along with the DEHP metabolite MEHP can be 
      selectively and sensitively quantified. Additionally, the method enables the 
      determination of the parent plasticizers TOTM and DEHP. The detection limits in 
      blood were found to range between 0.7-5.5mug/L for all TOTM analytes. Precision 
      and repeatability of the method were proven by relative standard deviations 
      between 0.9% and 8.7%. TOTM, an alternative plasticizer to DEHP, is already 
      increasingly used for medical devices. Nevertheless, data about the human 
      metabolism of TOTM are still limited. The presented method is the first one 
      enabling the simultaneous determination of the parent plasticizers TOTM and DEHP 
      together with their primary degradation products (DEHTM, MEHTM, MEHP) and can 
      thus be applied manifold including the investigation of the human metabolism of 
      TOTM.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Hollerer, Christine
AU  - Hollerer C
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 
      Erlangen, Germany.
FAU - Muller, Johannes
AU  - Muller J
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 
      Erlangen, Germany.
FAU - Goen, Thomas
AU  - Goen T
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 
      Erlangen, Germany.
FAU - Eckert, Elisabeth
AU  - Eckert E
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, University of Erlangen-Nuremberg, Schillerstrasse 25/29, 91054 
      Erlangen, Germany. Electronic address: elisabeth.eckert@fau.de.
LA  - eng
PT  - Journal Article
DEP - 20170710
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Benzoates)
RN  - 3319-31-1 (tri-(2-ethylhexyl)trimellitate)
SB  - IM
MH  - Animals
MH  - Benzoates/*blood/chemistry/*isolation & purification
MH  - Chromatography, Liquid/*methods
MH  - Horses
MH  - Humans
MH  - Isomerism
MH  - Limit of Detection
MH  - Linear Models
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Biomonitoring
OT  - Core shell material
OT  - Di-(2-ethylhexyl) phthalate
OT  - Human metabolites
OT  - Tri-(2-ethylhexyl) trimellitate
EDAT- 2017/07/25 06:00
MHDA- 2017/09/28 06:00
CRDT- 2017/07/24 06:00
PHST- 2017/03/14 00:00 [received]
PHST- 2017/07/05 00:00 [revised]
PHST- 2017/07/07 00:00 [accepted]
PHST- 2017/07/25 06:00 [pubmed]
PHST- 2017/09/28 06:00 [medline]
PHST- 2017/07/24 06:00 [entrez]
AID - S1570-0232(17)30426-9 [pii]
AID - 10.1016/j.jchromb.2017.07.014 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 1;1061-1062:153-162. doi: 
      10.1016/j.jchromb.2017.07.014. Epub 2017 Jul 10.