PMID- 28735348
OWN - NLM
STAT- MEDLINE
DCOM- 20180827
LR  - 20181113
IS  - 1432-1211 (Electronic)
IS  - 0093-7711 (Linking)
VI  - 70
IP  - 2
DP  - 2018 Feb
TI  - Analysis of the CD8(+) T cell anti-HIV activity in heterologous cell co-cultures 
      reveals the benefit of multiple HLA class I matches.
PG  - 99-113
LID - 10.1007/s00251-017-1021-7 [doi]
AB  - CD8(+) T lymphocytes can reduce the production of human immunodeficiency virus 1 
      (HIV-1) by CD4(+) T cells by cytotoxic and non-cytotoxic mechanisms. To 
      investigate the involvement of human leukocyte antigen (HLA) class I 
      compatibility in anti-HIV responses, we co-cultured primary CD8(+) T cells, 
      isolated from the peripheral blood of HIV-1-infected individuals, with panels of 
      autologous and heterologous acutely HIV-1-infected primary CD4(+) T cells. 
      Altogether, CD8(+) T cell anti-HIV activity was evaluated in more than 200 
      co-cultures. Marked heterogeneity in HIV-1 replication levels was observed among 
      the co-cultures sharing a common CD8(+) T cell source. The co-cultures that 
      exhibited greater than 50% reduction in HIV production were found to have 
      significantly increased numbers of matching HLA class I alleles (Yates 
      chi-square = 54.21; p < 0.001). With CD8(+) T cells from HIV controllers and 
      asymptomatic viremic individuals, matching HLA-B and/or HLA-C alleles were more 
      predictive of strong anti-HIV activity than matching HLA-A alleles. Overall, HLA 
      class I genotype matches were more closely associated with CD8(+) T cell anti-HIV 
      activity than supertype pairings. Antibodies against HLA class I and CD3 reduced 
      the CD8(+) T cell anti-HIV activity. Stimulated CD8(+) T cells exhibited 
      increased anti-HIV activity and reduced dependency on HLA compatibility. These 
      findings provide evidence that the maximal suppression of HIV replication by 
      CD8(+) T cells requires the recognition of multiple epitopes. These studies 
      provide insight for HIV vaccine development, and the analytic approach can be 
      useful for the functional characterization of HLA class I alleles and tentative 
      HLA class I supertypes.
FAU - Killian, M Scott
AU  - Killian MS
AUID- ORCID: 0000-0002-2012-3732
AD  - Basic Biomedical Sciences, Sanford School of Medicine, University of South 
      Dakota, 414 E. Clark St, Vermillion, SD, 57069, USA. m.scott.killian@gmail.com.
AD  - Department of Public Health, School of Health Sciences, University of South 
      Dakota, Vermillion, SD, 57069, USA. m.scott.killian@gmail.com.
FAU - Teque, Fernando
AU  - Teque F
AD  - Department of Medicine, University of California San Francisco, San Francisco, 
      CA, 94143, USA.
FAU - Sudhagoni, Ramu
AU  - Sudhagoni R
AD  - Department of Public Health, School of Health Sciences, University of South 
      Dakota, Vermillion, SD, 57069, USA.
LA  - eng
GR  - ID09-SF-058/California HIV/AIDS Research Program/International
GR  - 3P30AI027763-19S2/National Institute of Allergy and Infectious 
      Diseases/International
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170722
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology/*physiology
MH  - Coculture Techniques/methods
MH  - Genes, MHC Class I/immunology
MH  - HIV Infections/virology
MH  - HIV-1/immunology
MH  - HLA-B Antigens/genetics
MH  - HLA-C Antigens/genetics
MH  - Histocompatibility Antigens Class I/*genetics/*immunology
MH  - Humans
MH  - Primary Cell Culture
MH  - Virus Replication
OTO - NOTNLM
OT  - Antiviral immunity
OT  - CD8+ T cells
OT  - Elite controllers
OT  - HIV-1
OT  - HLA class I
OT  - Supertype
EDAT- 2017/07/25 06:00
MHDA- 2018/08/28 06:00
CRDT- 2017/07/24 06:00
PHST- 2017/05/23 00:00 [received]
PHST- 2017/07/17 00:00 [accepted]
PHST- 2017/07/25 06:00 [pubmed]
PHST- 2018/08/28 06:00 [medline]
PHST- 2017/07/24 06:00 [entrez]
AID - 10.1007/s00251-017-1021-7 [pii]
AID - 10.1007/s00251-017-1021-7 [doi]
PST - ppublish
SO  - Immunogenetics. 2018 Feb;70(2):99-113. doi: 10.1007/s00251-017-1021-7. Epub 2017 
      Jul 22.