PMID- 28735626
OWN - NLM
STAT- MEDLINE
DCOM- 20180409
LR  - 20181202
IS  - 1532-2823 (Electronic)
IS  - 0952-3278 (Linking)
VI  - 122
DP  - 2017 Jul
TI  - Differentiation of hMSC and hPDLSC induced by PGE2 or BMP-7 in 3D models.
PG  - 30-37
LID - S0952-3278(16)30246-0 [pii]
LID - 10.1016/j.plefa.2017.06.005 [doi]
AB  - Regenerative therapies of pathogenic tissue defects are gaining increasing 
      importance in periodontology. Among others, the osteogenic effect of BMP-7 seems 
      to play a major role in the development of teeth and alveolar bone. Human 
      periodontal ligament stem cells (hPDLSC), as well as human mesenchymal stem cells 
      (hMSC), show the ability to differentiate into various types of tissues. 
      Regarding prostaglandin E2, many studies have confirmed that it is involved in 
      the inflammation associated to periodontitis stimulating osteoclasts, which 
      ultimately leads to resorption of tooth supporting bone. Herein, we aimed to 
      investigate how PGE2 influences regenerative processes. The influence of PGE2 and 
      BMP-7 on the osteogenic differentiation of hMSC and hPDLSC was determined in a 3D 
      cell culture model using qRT-PCR, immunocytochemistry and REM. BMP-7 enhanced the 
      expression of osteogenic markers in hMSC and lowered it in hPDLSC-TERT. BMP-7 had 
      a lower osteogenic effect on hPDLSC-hTERT than on hMSC, while PGE2 decreases the 
      osteogenic differentiation in both cell types, thus, inhibiting anabolic 
      processes. Both cell types presented good proliferation and adhesion onto the 
      scaffolds. The well-developed structural morphology and the support of osteogenic 
      differentiation suggest that the scaffolds are potential candidate materials for 
      bone regeneration. The positivity for Cap in hPDLSC and more in hMSC 
      immunostaining samples indicates the initiation of neocementogenesis as part of 
      periodontal regeneration. In conclusion, BMP7, in particular combined with MSC, 
      seems to have a favourable application also in periodontal regeneration. Our 
      results show that inflammation plays an important role in periodontal 
      regeneration. PGE2 is a key mediator, which stimulates bone resorption also via a 
      mechanism involving the inhibition of osteogenic differentiation of MSC as well 
      as PDLSC. Therefore, regenerative approaches should always be conducted in 
      combination with anti-inflammatory measures oriented to control inflammation.
CI  - Copyright (c) 2017. Published by Elsevier Ltd.
FAU - Ern, Christina
AU  - Ern C
AD  - Department of Operative Dentistry and Periodontology, University Hospital, 
      Ludwig-Maximilians-Universitat Munchen, Germany. Electronic address: 
      chrisern@dent.med.uni-muenchen.de.
FAU - Berger, Tamara
AU  - Berger T
AD  - Department of Operative Dentistry and Periodontology, University Hospital, 
      Ludwig-Maximilians-Universitat Munchen, Germany; Max Planck Institute of 
      Psychiatry, Munich, Germany. Electronic address: berger_tamara@gmx.de.
FAU - Frasheri, Iris
AU  - Frasheri I
AD  - Department of Operative Dentistry and Periodontology, University Hospital, 
      Ludwig-Maximilians-Universitat Munchen, Germany. Electronic address: 
      irisfrasheri@yahoo.it.
FAU - Heym, Richard
AU  - Heym R
AD  - Department of Operative Dentistry and Periodontology, University Hospital, 
      Ludwig-Maximilians-Universitat Munchen, Germany. Electronic address: 
      rheym@dent.med.uni-muenchen.de.
FAU - Hickel, Reinhard
AU  - Hickel R
AD  - Department of Operative Dentistry and Periodontology, University Hospital, 
      Ludwig-Maximilians-Universitat Munchen, Germany. Electronic address: 
      hickel@dent.med.uni-muenchen.de.
FAU - Folwaczny, Matthias
AU  - Folwaczny M
AD  - Department of Operative Dentistry and Periodontology, University Hospital, 
      Ludwig-Maximilians-Universitat Munchen, Germany. Electronic address: 
      mfolwa@dent.med.uni-muenchen.de.
LA  - eng
PT  - Journal Article
DEP - 20170615
PL  - Scotland
TA  - Prostaglandins Leukot Essent Fatty Acids
JT  - Prostaglandins, leukotrienes, and essential fatty acids
JID - 8802730
RN  - 0 (BMP7 protein, human)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Bone Morphogenetic Protein 7/*pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Dinoprostone/*pharmacology
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism/pathology
MH  - Periodontal Ligament/*metabolism/pathology
OTO - NOTNLM
OT  - BMP-7
OT  - Mesenchymal stem cell
OT  - Periodontal ligament stem cell
OT  - Periodontitis
OT  - Scaffold
OT  - hTERT
EDAT- 2017/07/25 06:00
MHDA- 2018/04/10 06:00
CRDT- 2017/07/25 06:00
PHST- 2016/12/02 00:00 [received]
PHST- 2017/04/06 00:00 [revised]
PHST- 2017/06/12 00:00 [accepted]
PHST- 2017/07/25 06:00 [entrez]
PHST- 2017/07/25 06:00 [pubmed]
PHST- 2018/04/10 06:00 [medline]
AID - S0952-3278(16)30246-0 [pii]
AID - 10.1016/j.plefa.2017.06.005 [doi]
PST - ppublish
SO  - Prostaglandins Leukot Essent Fatty Acids. 2017 Jul;122:30-37. doi: 
      10.1016/j.plefa.2017.06.005. Epub 2017 Jun 15.