PMID- 28738228
OWN - NLM
STAT- MEDLINE
DCOM- 20180504
LR  - 20180504
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 99
DP  - 2018 Jan 15
TI  - Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme 
      immobilization on an electrode.
PG  - 56-61
LID - S0956-5663(17)30493-1 [pii]
LID - 10.1016/j.bios.2017.07.044 [doi]
AB  - A tetrameric streptavidin (SA)-appended LPETG tag was site-specifically linked to 
      azido-containing tri-glycine via sortase A catalysis and the resulting 
      azido-modified SA (SA-N3) was retained in the biotin-binding pocket. SA-N3 was 
      polymerized with dibenzylcyclooctyne-modified branched poly(ethyleneglycol) 
      (DBCO-PEG) using azido-modified branched PEG (N3-PEG) as a spacer via copper-free 
      click chemistry. The resulting SA-based hydrogel exhibited gel-like mechanical 
      properties and could immobilize biotin-modified molecules through biotin-SA 
      affinity. Glucose dehydrogenase (GDH) was immobilized in the SA-based hydrogel, 
      and the hydrogel was then coated on a glassy carbon electrode (GCE) and used for 
      the biocatalytic oxidation of glucose. The designed GCE exhibited better 
      performance and stability compared with GDH chemically adsorbed onto a GCE. In 
      addition, the designed GCE anode and a Pt-carbon cathode were assembled into a 
      glucose/O(2) fuel cell that provided a maximum power density and open circuit 
      voltage of 11.8+/-0.56microWcm(-2) and 0.17V, respectively.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Matsumoto, Takuya
AU  - Matsumoto T
AD  - Graduate School of Science, Technology and Innovation, Kobe University, 1-1 
      Rokkodaicho, Nada, Kobe 657-8501, Japan.
FAU - Isogawa, Yuki
AU  - Isogawa Y
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
FAU - Tanaka, Tsutomu
AU  - Tanaka T
AD  - Department of Chemical Science and Engineering, Graduate School of Engineering, 
      Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan. Electronic address: 
      tanaka@kitty.kobe-u.ac.jp.
FAU - Kondo, Akihiko
AU  - Kondo A
AD  - Graduate School of Science, Technology and Innovation, Kobe University, 1-1 
      Rokkodaicho, Nada, Kobe 657-8501, Japan; Department of Chemical Science and 
      Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, 
      Nada, Kobe 657-8501, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170719
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Enzymes, Immobilized)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 7440-44-0 (Carbon)
RN  - 9013-20-1 (Streptavidin)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - *Biosensing Techniques
MH  - Carbon/chemistry
MH  - *Click Chemistry
MH  - Enzymes, Immobilized/chemistry
MH  - Glucose/chemistry/*isolation & purification
MH  - Glucose 1-Dehydrogenase/chemistry
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry
MH  - Oxidation-Reduction
MH  - Streptavidin/chemistry
OTO - NOTNLM
OT  - Bioanode
OT  - Hydrogel
OT  - Sortase A
OT  - Streptavidin
EDAT- 2017/07/25 06:00
MHDA- 2018/05/05 06:00
CRDT- 2017/07/25 06:00
PHST- 2017/04/20 00:00 [received]
PHST- 2017/07/14 00:00 [revised]
PHST- 2017/07/18 00:00 [accepted]
PHST- 2017/07/25 06:00 [pubmed]
PHST- 2018/05/05 06:00 [medline]
PHST- 2017/07/25 06:00 [entrez]
AID - S0956-5663(17)30493-1 [pii]
AID - 10.1016/j.bios.2017.07.044 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2018 Jan 15;99:56-61. doi: 10.1016/j.bios.2017.07.044. Epub 
      2017 Jul 19.