PMID- 28739272
OWN - NLM
STAT- MEDLINE
DCOM- 20171204
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1513
DP  - 2017 Sep 1
TI  - An on-line analytical approach for detecting haptens in Shuang-huang-lian powder 
      injection.
PG  - 126-139
LID - S0021-9673(17)31014-2 [pii]
LID - 10.1016/j.chroma.2017.07.031 [doi]
AB  - Shuang-huang-lian powder injection (SHLPI) is a traditional Chinese medicine 
      injection (TCMI) frequently used in the clinical treatment of faucitis, 
      bronchitis, and other viral and bacterial infections of upper respiratory tract. 
      However, its allergenic reactions, being the main adverse effects (AEs) of SHLPI, 
      have been a serious problem of its clinical safety. This problem has not been 
      solved due to short of methods for detecting haptens in complex TCMIs. In this 
      study, an on-line high-performance liquid chromatography-diode-array 
      detector-mass spectrometry combined with bovine serum albumin-fluorescence 
      detector (HPLC-DAD-MS-BSA-FLD) system was established for the first time, 
      validated and applied for identification of haptens in SHLPI. Fourteen of 35 
      identified compounds showed BSA binding activity, and they were six flavonoids, 
      six caffeoylquinic acids (CQAs), and two phenylethanoid glycosides. The 
      structure-activity relationships of 10 active components were studied, and their 
      ability of sensitization together with that of two CQAs were further verified by 
      ELISA assay. It was found that 10 compounds had sensitization, and flavonoids 
      showed stronger sensitizability than CQAs while the diCQAs were slightly stronger 
      than caffeoylquinic acids. The system was validated using 3-CQA as a positive 
      control, and was proved to have good reproducibility, stability, precision 
      (RSD<0.1%) and linearity (R(2)>0.9993). This online system is fast, sensitive and 
      efficient for screening haptens in traditional Chinese medicine injection (TCMI), 
      provides a new approach to reveal the chemical basis of haptens in TCMIs.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Wang, Hong
AU  - Wang H
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Zhang, Xiaotian
AU  - Zhang X
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Wang, Daidong
AU  - Wang D
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Sun, Hongyang
AU  - Sun H
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Lan, Yuexiang
AU  - Lan Y
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Jiang, Haixiu
AU  - Jiang H
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Chen, Shizhong
AU  - Chen S
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. 
      Electronic address: chenshizhong66@163.com.
FAU - Lin, Zongtao
AU  - Lin Z
AD  - Department of Pharmaceutical Sciences, University of Tennessee Health Science 
      Center, Memphis, TN 38163, United States. Electronic address: zlin8@uthsc.edu.
LA  - eng
PT  - Journal Article
DEP - 20170713
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Haptens)
RN  - 0 (Organic Chemicals)
RN  - 0 (Powders)
RN  - 0 (Solvents)
RN  - 0 (shuang-huang-lian)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Animals
MH  - Calibration
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Haptens/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Injections
MH  - Limit of Detection
MH  - Mass Spectrometry
MH  - *Online Systems
MH  - Organic Chemicals/chemistry
MH  - Powders
MH  - Protein Binding
MH  - Reproducibility of Results
MH  - Serum Albumin, Bovine/metabolism
MH  - Sodium Chloride/chemistry
MH  - Solvents/chemistry
MH  - Structure-Activity Relationship
MH  - Temperature
OTO - NOTNLM
OT  - Anaphylactoid reaction
OT  - ELISA
OT  - Haptens
OT  - On-line method
OT  - Shuang-huang-lian powder injection
EDAT- 2017/07/26 06:00
MHDA- 2017/12/05 06:00
CRDT- 2017/07/26 06:00
PHST- 2017/02/21 00:00 [received]
PHST- 2017/06/20 00:00 [revised]
PHST- 2017/07/10 00:00 [accepted]
PHST- 2017/07/26 06:00 [pubmed]
PHST- 2017/12/05 06:00 [medline]
PHST- 2017/07/26 06:00 [entrez]
AID - S0021-9673(17)31014-2 [pii]
AID - 10.1016/j.chroma.2017.07.031 [doi]
PST - ppublish
SO  - J Chromatogr A. 2017 Sep 1;1513:126-139. doi: 10.1016/j.chroma.2017.07.031. Epub 
      2017 Jul 13.