PMID- 28739633
OWN - NLM
STAT- MEDLINE
DCOM- 20180430
LR  - 20190214
IS  - 1479-6813 (Electronic)
IS  - 0952-5041 (Linking)
VI  - 59
IP  - 3
DP  - 2017 Oct
TI  - Claudin-8d is a cortisol-responsive barrier protein in the gill epithelium of 
      trout.
PG  - 299-310
LID - 10.1530/JME-17-0108 [doi]
AB  - The influence of claudin (Cldn) 8 tight junction (TJ) proteins on 
      cortisol-mediated alterations in gill epithelium permeability was examined using 
      a primary cultured trout gill epithelium model. Genes encoding three Cldn-8 
      proteins (cldn-8b, -8c and -8d) have been identified in trout and all are 
      expressed in the model gill epithelium. Cortisol treatment 'tightened' the gill 
      epithelium, as indicated by increased transepithelial resistance (TER) and 
      reduced paracellular [(3)H]polyethylene glycol (MW 400 Da; PEG-400) flux. This 
      occurred in association with elevated cldn-8d mRNA abundance, but no alterations 
      in cldn-8b and -8c mRNA abundance were observed. Transcriptional knockdown (KD) 
      of cldn-8d inhibited a cortisol-induced increase in Cldn-8d abundance and reduced 
      the 'epithelium tightening' effect of cortisol in association with increased 
      paracellular PEG-400 flux. Under simulated in vivo conditions (i.e. apical 
      freshwater), cldn-8d KD hindered a cortisol-mediated reduction in basolateral to 
      apical Na(+) and Cl(-) flux (i.e. reduced the ability of cortisol to mitigate ion 
      loss). However, cldn-8d KD did not abolish the tightening effect of cortisol on 
      the gill epithelium. This is likely due, in part, to the effect of cortisol on 
      genes encoding other TJ proteins, which in some cases appeared to exhibit a 
      compensatory response. Data support the idea that Cldn-8d is a barrier protein of 
      the gill epithelium TJ that contributes significantly to corticosteroid-mediated 
      alterations in gill epithelium permeability.
CI  - (c) 2017 Society for Endocrinology.
FAU - Kolosov, Dennis
AU  - Kolosov D
AD  - Department of BiologyYork University, Toronto, Ontario, Canada.
FAU - Kelly, Scott P
AU  - Kelly SP
AD  - Department of BiologyYork University, Toronto, Ontario, Canada spk@yorku.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170724
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Claudins)
RN  - 0 (RNA Isoforms)
RN  - 0 (RNA, Messenger)
RN  - HJ6C19SU7L (claudin 8)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Cell Membrane Permeability/drug effects
MH  - Claudins/genetics/*metabolism
MH  - Epithelium/*metabolism
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Gills/*metabolism
MH  - Hydrocortisone/metabolism/pharmacology
MH  - Oncorhynchus mykiss/*physiology
MH  - RNA Interference
MH  - RNA Isoforms
MH  - RNA, Messenger/genetics/metabolism
MH  - Tight Junctions/metabolism
OTO - NOTNLM
OT  - corticosteroid
OT  - fish gill
OT  - osmoregulation
OT  - paracellular permeability
OT  - tight junction
EDAT- 2017/07/26 06:00
MHDA- 2018/05/01 06:00
CRDT- 2017/07/26 06:00
PHST- 2017/07/11 00:00 [received]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/07/26 06:00 [pubmed]
PHST- 2018/05/01 06:00 [medline]
PHST- 2017/07/26 06:00 [entrez]
AID - JME-17-0108 [pii]
AID - 10.1530/JME-17-0108 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 2017 Oct;59(3):299-310. doi: 10.1530/JME-17-0108. Epub 2017 Jul 
      24.