PMID- 28739796
OWN - NLM
STAT- MEDLINE
DCOM- 20180530
LR  - 20181202
IS  - 1098-6596 (Electronic)
IS  - 0066-4804 (Print)
IS  - 0066-4804 (Linking)
VI  - 61
IP  - 10
DP  - 2017 Oct
TI  - Multicenter, International Study of MIC/MEC Distributions for Definition of 
      Epidemiological Cutoff Values for Sporothrix Species Identified by Molecular 
      Methods.
LID - 10.1128/AAC.01057-17 [doi]
LID - e01057-17
AB  - Clinical and Laboratory Standards Institute (CLSI) conditions for testing the 
      susceptibilities of pathogenic Sporothrix species to antifungal agents are based 
      on a collaborative study that evaluated five clinically relevant isolates of 
      Sporothrixschenckii sensu lato and some antifungal agents. With the advent of 
      molecular identification, there are two basic needs: to confirm the suitability 
      of these testing conditions for all agents and Sporothrix species and to 
      establish species-specific epidemiologic cutoff values (ECVs) or breakpoints 
      (BPs) for the species. We collected available CLSI MICs/minimal effective 
      concentrations (MECs) of amphotericin B, five triazoles, terbinafine, 
      flucytosine, and caspofungin for 301 Sporothrix schenckii sensu stricto, 486 S. 
      brasiliensis, 75 S. globosa, and 13 S. mexicana molecularly identified isolates. 
      Data were obtained in 17 independent laboratories (Australia, Europe, India, 
      South Africa, and South and North America) using conidial inoculum suspensions 
      and 48 to 72 h of incubation at 35 degrees C. Sufficient and suitable data (modal MICs 
      within 2-fold concentrations) allowed the proposal of the following ECVs for S. 
      schenckii and S. brasiliensis, respectively: amphotericin B, 4 and 4 mug/ml; 
      itraconazole, 2 and 2 mug/ml; posaconazole, 2 and 2 mug/ml; and voriconazole, 64 
      and 32 mug/ml. Ketoconazole and terbinafine ECVs for S. brasiliensis were 2 and 
      0.12 mug/ml, respectively. Insufficient or unsuitable data precluded the 
      calculation of ketoconazole and terbinafine (or any other antifungal agent) ECVs 
      for S. schenckii, as well as ECVs for S. globosa and S. mexicana These ECVs could 
      aid the clinician in identifying potentially resistant isolates (non-wild type) 
      less likely to respond to therapy.
CI  - Copyright (c) 2017 American Society for Microbiology.
FAU - Espinel-Ingroff, A
AU  - Espinel-Ingroff A
AD  - VCU Medical Center, Richmond, Virginia, USA victoria.ingroff@vcuhealth.org.
FAU - Abreu, D P B
AU  - Abreu DPB
AD  - Universidade Federal Rural do Rio de Janeiro, Seropedica, Brazil.
FAU - Almeida-Paes, R
AU  - Almeida-Paes R
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, 
      Laboratorio de Micologia, Rio de Janeiro, RJ, Brazil.
FAU - Brilhante, R S N
AU  - Brilhante RSN
AD  - Specialized Medical Mycology Center, Federal University of Ceara, Fortaleza-CE, 
      Brazil.
FAU - Chakrabarti, A
AU  - Chakrabarti A
AD  - Department of Medical Microbiology, Postgraduate Institute of Medical Education 
      and Research, Chandigarh, India.
FAU - Chowdhary, A
AU  - Chowdhary A
AUID- ORCID: 0000-0002-2028-7462
AD  - Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of 
      Delhi, Delhi, India.
FAU - Hagen, F
AU  - Hagen F
AUID- ORCID: 0000-0002-5622-1916
AD  - Canisius Wilhelmina Hospital, Centre of Expertise in Mycology, Nijmegen, The 
      Netherlands.
FAU - Cordoba, S
AU  - Cordoba S
AD  - Departamento Micologia, Instituto Nacional de Enfermedades Infecciosas Dr. C. G. 
      Malbran, Buenos Aires, Argentina.
FAU - Gonzalez, G M
AU  - Gonzalez GM
AD  - Universidad Autonoma de Nuevo Leon, Monterrey, Nuevo Leon, Mexico.
FAU - Govender, N P
AU  - Govender NP
AUID- ORCID: 0000-0001-7869-9462
AD  - National Institute for Communicable Diseases and University of the Witwatersrand, 
      Johannesburg, South Africa.
FAU - Guarro, J
AU  - Guarro J
AD  - Mycology Unit Medical School, Universitat Rovira i Virgili, Reus, Spain.
FAU - Johnson, E M
AU  - Johnson EM
AD  - Mycology Reference Laboratory, Public Health England, Bristol, United Kingdom.
FAU - Kidd, S E
AU  - Kidd SE
AD  - National Mycology Reference Centre, SA Pathology, Adelaide, Australia.
FAU - Pereira, S A
AU  - Pereira SA
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, 
      Laboratorio de Micologia, Rio de Janeiro, RJ, Brazil.
FAU - Rodrigues, A M
AU  - Rodrigues AM
AUID- ORCID: 0000-0003-0891-4563
AD  - Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
FAU - Rozental, S
AU  - Rozental S
AD  - Instituto de Biofisica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 
      Brazil.
FAU - Szeszs, M W
AU  - Szeszs MW
AD  - Instituto Adolfo Lutz, Aracatuba, Rio Claro Laboratories, Sao Paulo, Brazil.
FAU - Balleste Alaniz, R
AU  - Balleste Alaniz R
AD  - Departamento de Laboratorio Clinico, Hospital de Clinicas Dr. M. Quintela, 
      Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay.
FAU - Bonifaz, A
AU  - Bonifaz A
AD  - Hospital General de Mexico, Mexico City, Mexico.
FAU - Bonfietti, L X
AU  - Bonfietti LX
AD  - Instituto Adolfo Lutz, Aracatuba, Rio Claro Laboratories, Sao Paulo, Brazil.
FAU - Borba-Santos, L P
AU  - Borba-Santos LP
AD  - Instituto de Biofisica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 
      Brazil.
FAU - Capilla, J
AU  - Capilla J
AD  - Mycology Unit Medical School, Universitat Rovira i Virgili, Reus, Spain.
FAU - Colombo, A L
AU  - Colombo AL
AD  - Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
FAU - Dolande, M
AU  - Dolande M
AD  - Instituto Nacional de Higiene Rafael Rangel, Caracas, Venezuela.
FAU - Isla, M G
AU  - Isla MG
AD  - Departamento Micologia, Instituto Nacional de Enfermedades Infecciosas Dr. C. G. 
      Malbran, Buenos Aires, Argentina.
FAU - Melhem, M S C
AU  - Melhem MSC
AD  - Instituto Adolfo Lutz, Aracatuba, Rio Claro Laboratories, Sao Paulo, Brazil.
FAU - Mesa-Arango, A C
AU  - Mesa-Arango AC
AD  - Grupo de Investigacion Dermatologica, Universidad de Antioquia, Medellin, 
      Colombia.
FAU - Oliveira, M M E
AU  - Oliveira MME
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, 
      Laboratorio de Micologia, Rio de Janeiro, RJ, Brazil.
FAU - Panizo, M M
AU  - Panizo MM
AD  - Instituto Nacional de Higiene Rafael Rangel, Caracas, Venezuela.
FAU - Pires de Camargo, Z
AU  - Pires de Camargo Z
AD  - Universidade Federal de Sao Paulo, Sao Paulo, Brazil.
FAU - Zancope-Oliveira, R M
AU  - Zancope-Oliveira RM
AD  - Fundacao Oswaldo Cruz-Fiocruz, Instituto Nacional de Infectologia Evandro Chagas, 
      Laboratorio de Micologia, Rio de Janeiro, RJ, Brazil.
FAU - Meis, J F
AU  - Meis JF
AUID- ORCID: 0000-0003-3253-6080
AD  - Canisius Wilhelmina Hospital, Centre of Expertise in Mycology, Nijmegen, The 
      Netherlands.
FAU - Turnidge, J
AU  - Turnidge J
AD  - University of Adelaide, Adelaide, Australia.
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
DEP - 20170922
PL  - United States
TA  - Antimicrob Agents Chemother
JT  - Antimicrobial agents and chemotherapy
JID - 0315061
RN  - 0 (Antifungal Agents)
RN  - 0 (Echinocandins)
RN  - 0 (Lipopeptides)
RN  - 0 (Naphthalenes)
RN  - 0 (Triazoles)
RN  - 7XU7A7DROE (Amphotericin B)
RN  - D83282DT06 (Flucytosine)
RN  - F0XDI6ZL63 (Caspofungin)
RN  - G7RIW8S0XP (Terbinafine)
SB  - IM
MH  - Amphotericin B/*pharmacology
MH  - Antifungal Agents/*pharmacology
MH  - Caspofungin
MH  - Echinocandins/*pharmacology
MH  - Flucytosine/*pharmacology
MH  - Humans
MH  - Lipopeptides/*pharmacology
MH  - Microbial Sensitivity Tests
MH  - Naphthalenes/*pharmacology
MH  - Sporothrix/classification/*drug effects/isolation & purification
MH  - Sporotrichosis/*drug therapy
MH  - Terbinafine
MH  - Triazoles/*pharmacology
PMC - PMC5610517
OTO - NOTNLM
OT  - ECVs
OT  - Sporothrix
OT  - antifungal resistance
OT  - molecular methods
EDAT- 2017/07/26 06:00
MHDA- 2018/05/31 06:00
PMCR- 2018/03/22
CRDT- 2017/07/26 06:00
PHST- 2017/05/22 00:00 [received]
PHST- 2017/07/20 00:00 [accepted]
PHST- 2017/07/26 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
PHST- 2017/07/26 06:00 [entrez]
PHST- 2018/03/22 00:00 [pmc-release]
AID - AAC.01057-17 [pii]
AID - 01057-17 [pii]
AID - 10.1128/AAC.01057-17 [doi]
PST - epublish
SO  - Antimicrob Agents Chemother. 2017 Sep 22;61(10):e01057-17. doi: 
      10.1128/AAC.01057-17. Print 2017 Oct.