PMID- 28740863
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20190701
IS  - 2375-2548 (Electronic)
IS  - 2375-2548 (Linking)
VI  - 3
IP  - 7
DP  - 2017 Jul
TI  - A nanosecond time-resolved XFEL analysis of structural changes associated with CO 
      release from cytochrome c oxidase.
PG  - e1603042
LID - 10.1126/sciadv.1603042 [doi]
LID - e1603042
AB  - Bovine cytochrome c oxidase (CcO), a 420-kDa membrane protein, pumps protons 
      using electrostatic repulsion between protons transferred through a water channel 
      and net positive charges created by oxidation of heme a (Fe (a) ) for reduction 
      of O(2) at heme a(3) (Fe (a3)). For this process to function properly, timing is 
      essential: The channel must be closed after collection of the protons to be 
      pumped and before Fe (a) oxidation. If the channel were to remain open, 
      spontaneous backflow of the collected protons would occur. For elucidation of the 
      channel closure mechanism, the opening of the channel, which occurs upon release 
      of CO from CcO, is investigated by newly developed time-resolved x-ray 
      free-electron laser and infrared techniques with nanosecond time resolution. The 
      opening process indicates that Cu(B) senses completion of proton collection and 
      binds O(2) before binding to Fe (a3) to close the water channel using a 
      conformational relay system, which includes Cu(B), heme a(3), and a transmembrane 
      helix, to block backflow of the collected protons.
FAU - Shimada, Atsuhiro
AU  - Shimada A
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
FAU - Kubo, Minoru
AU  - Kubo M
AUID- ORCID: 0000-0002-1470-942X
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
AD  - Japan Science and Technology Agency, Precursory Research for Embryonic Science 
      and Technology (PRESTO), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
FAU - Baba, Seiki
AU  - Baba S
AD  - Japan Synchrotron Radiation Research Institute, 1-1-1, Kouto, Sayo-cho, Sayo-gun, 
      Hyogo 679-5198, Japan.
FAU - Yamashita, Keitaro
AU  - Yamashita K
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
FAU - Hirata, Kunio
AU  - Hirata K
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
AD  - Japan Science and Technology Agency, Precursory Research for Embryonic Science 
      and Technology (PRESTO), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
FAU - Ueno, Go
AU  - Ueno G
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
FAU - Nomura, Takashi
AU  - Nomura T
AUID- ORCID: 0000-0002-4613-7403
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
FAU - Kimura, Tetsunari
AU  - Kimura T
AUID- ORCID: 0000-0003-1650-7298
AD  - Graduate School of Science, Kobe University, 1-1 Rokkodai, Nada, Kobe, Hyogo 
      657-8501, Japan.
FAU - Shinzawa-Itoh, Kyoko
AU  - Shinzawa-Itoh K
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
FAU - Baba, Junpei
AU  - Baba J
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
FAU - Hatano, Keita
AU  - Hatano K
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
FAU - Eto, Yuki
AU  - Eto Y
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
FAU - Miyamoto, Akari
AU  - Miyamoto A
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
FAU - Murakami, Hironori
AU  - Murakami H
AD  - Japan Synchrotron Radiation Research Institute, 1-1-1, Kouto, Sayo-cho, Sayo-gun, 
      Hyogo 679-5198, Japan.
FAU - Kumasaka, Takashi
AU  - Kumasaka T
AD  - Japan Synchrotron Radiation Research Institute, 1-1-1, Kouto, Sayo-cho, Sayo-gun, 
      Hyogo 679-5198, Japan.
FAU - Owada, Shigeki
AU  - Owada S
AUID- ORCID: 0000-0002-1451-7612
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
FAU - Tono, Kensuke
AU  - Tono K
AUID- ORCID: 0000-0003-1218-3759
AD  - Japan Synchrotron Radiation Research Institute, 1-1-1, Kouto, Sayo-cho, Sayo-gun, 
      Hyogo 679-5198, Japan.
FAU - Yabashi, Makina
AU  - Yabashi M
AUID- ORCID: 0000-0002-2472-1684
AD  - Japan Synchrotron Radiation Research Institute, 1-1-1, Kouto, Sayo-cho, Sayo-gun, 
      Hyogo 679-5198, Japan.
FAU - Yamaguchi, Yoshihiro
AU  - Yamaguchi Y
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
FAU - Yanagisawa, Sachiko
AU  - Yanagisawa S
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
FAU - Sakaguchi, Miyuki
AU  - Sakaguchi M
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
FAU - Ogura, Takashi
AU  - Ogura T
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      1-1-1 Kouto, Sayo, Hyogo 679-5148, Japan.
FAU - Komiya, Ryo
AU  - Komiya R
AD  - Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, 
      Yokohama 223-8522, Japan.
FAU - Yan, Jiwang
AU  - Yan J
AD  - Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, 
      Yokohama 223-8522, Japan.
FAU - Yamashita, Eiki
AU  - Yamashita E
AD  - Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 
      565-0871, Japan.
FAU - Yamamoto, Masaki
AU  - Yamamoto M
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
FAU - Ago, Hideo
AU  - Ago H
AD  - RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
FAU - Yoshikawa, Shinya
AU  - Yoshikawa S
AUID- ORCID: 0000-0002-6020-0974
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
FAU - Tsukihara, Tomitake
AU  - Tsukihara T
AD  - Picobiology Institute, Graduate School of Life Science, University of Hyogo, 
      3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
AD  - Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 
      565-0871, Japan.
AD  - Japan Science and Technology Agency, Core Research for Evolutional Science and 
      Technology (CREST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170714
PL  - United States
TA  - Sci Adv
JT  - Science advances
JID - 101653440
RN  - 42VZT0U6YR (Heme)
RN  - 7U1EE4V452 (Carbon Monoxide)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Carbon Monoxide/*chemistry/*metabolism
MH  - Electron Transport Complex IV/*chemistry/*metabolism
MH  - Heme/chemistry/metabolism
MH  - Hydrogen Bonding
MH  - Models, Molecular
MH  - Molecular Conformation
MH  - Molecular Structure
MH  - Oxidation-Reduction
MH  - Photolysis
MH  - Structure-Activity Relationship
PMC - PMC5510965
EDAT- 2017/07/26 06:00
MHDA- 2019/07/02 06:00
PMCR- 2017/07/14
CRDT- 2017/07/26 06:00
PHST- 2016/12/02 00:00 [received]
PHST- 2017/06/14 00:00 [accepted]
PHST- 2017/07/26 06:00 [entrez]
PHST- 2017/07/26 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
PHST- 2017/07/14 00:00 [pmc-release]
AID - 1603042 [pii]
AID - 10.1126/sciadv.1603042 [doi]
PST - epublish
SO  - Sci Adv. 2017 Jul 14;3(7):e1603042. doi: 10.1126/sciadv.1603042. eCollection 2017 
      Jul.