PMID- 28741259
OWN - NLM
STAT- MEDLINE
DCOM- 20180418
LR  - 20181113
IS  - 1559-0755 (Electronic)
IS  - 0257-277X (Print)
IS  - 0257-277X (Linking)
VI  - 65
IP  - 4
DP  - 2017 Aug
TI  - Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice 
      triggers a B lymphocyte humoral response.
PG  - 957-968
LID - 10.1007/s12026-017-8936-9 [doi]
AB  - Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by 
      increased autoantibody production that leads to multiple tissue injuries. 
      Dendritic cells (DCs) are important orchestrators of immune responses and key 
      components in fine-tuning the balance between tolerance and immunity. However, 
      their role in autoimmune disorders such as SLE remains uncertain. We analyzed the 
      contribution of DCs in triggering SLE by adoptively transferring splenic DCs from 
      aged autoimmune [NZBxNZW]F1 (BWF1) mice to young healthy BWF1 mice. We observed 
      that the transfer of DCs from autoimmune mice to pre-autoimmune mice induced high 
      autoantibody titers in the serum of recipient mice. Moreover, autoimmune DCs from 
      aged BWF1 mice were crucial for the expansion and differentiation of plasmablasts 
      and CD5(+) B cells or B1-like cells in the peripheral blood, and spleen of 
      recipient BWF1 mice, a phenomenon that is observed in autoimmune BWF1 mice. On 
      the other hand, DCs from aged BWF1 mice participated in the expansion and 
      differentiation of DCs and IFN-gamma-producing T cells. These results reveal that DCs 
      from autoimmune BWF1 mice exhibit functional and phenotypic characteristics that 
      allow them to trigger B cell hyperactivation, as well as DC and T cell expansion 
      and differentiation, thereby promoting an exacerbated humoral response in 
      lupus-prone mice.
FAU - Sauma, Daniela
AU  - Sauma D
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Crisostomo, Natalia
AU  - Crisostomo N
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Fuentes, Camila
AU  - Fuentes C
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Gleisner, Maria Alejandra
AU  - Gleisner MA
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Hidalgo, Yessia
AU  - Hidalgo Y
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Fuenzalida, Maria Jose
AU  - Fuenzalida MJ
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Rosemblatt, Mario
AU  - Rosemblatt M
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
AD  - Fundacion Ciencia & Vida, Santiago, Chile.
AD  - Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile.
FAU - Bono, Maria Rosa
AU  - Bono MR
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile. mrbono@uchile.cl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunol Res
JT  - Immunologic research
JID - 8611087
RN  - 0 (Autoantibodies)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adoptive Transfer
MH  - Aging/*physiology
MH  - Animals
MH  - Autoantibodies/metabolism
MH  - B-Lymphocytes/*immunology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/transplantation
MH  - Humans
MH  - Immunity, Humoral
MH  - Interferon-gamma/metabolism
MH  - Lupus Erythematosus, Systemic/*immunology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred NZB
MH  - Spleen/*pathology
MH  - T-Lymphocytes/*immunology
PMC - PMC5544790
OTO - NOTNLM
OT  - Autoimmunity
OT  - B lymphocytes
OT  - Dendritic cells
OT  - Humoral response
OT  - Systemic lupus erythematosus
COIS- Animal work was carried out under the institutional regulations of the Fundacion 
      Ciencia & Vida and was approved locally by the ethical review committee of the 
      Facultad de Ciencias, Universidad de Chile. FUNDING: Funded by FONDECYT 1140431 
      (MRB), FONDECYT 1121478 (DS), PAI 791100009 (MRB, DS), FONDEQUIP/EQM114137, and 
      CONICYT PFB-16 (MR). CONFLICT OF INTEREST: The authors declare that they have no 
      conflict of interest.
EDAT- 2017/07/26 06:00
MHDA- 2018/04/19 06:00
PMCR- 2017/07/25
CRDT- 2017/07/26 06:00
PHST- 2017/07/26 06:00 [pubmed]
PHST- 2018/04/19 06:00 [medline]
PHST- 2017/07/26 06:00 [entrez]
PHST- 2017/07/25 00:00 [pmc-release]
AID - 10.1007/s12026-017-8936-9 [pii]
AID - 8936 [pii]
AID - 10.1007/s12026-017-8936-9 [doi]
PST - ppublish
SO  - Immunol Res. 2017 Aug;65(4):957-968. doi: 10.1007/s12026-017-8936-9.