PMID- 28742001
OWN - NLM
STAT- MEDLINE
DCOM- 20170906
LR  - 20191209
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 98
IP  - 8
DP  - 2017 Aug
TI  - Molecular cloning of porcine Siglec-3, Siglec-5 and Siglec-10, and identification 
      of Siglec-10 as an alternative receptor for porcine reproductive and respiratory 
      syndrome virus (PRRSV).
PG  - 2030-2042
LID - 10.1099/jgv.0.000859 [doi]
AB  - In recent years, several entry mediators have been characterized for porcine 
      reproductive and respiratory syndrome virus (PRRSV). Porcine sialoadhesin [pSn, 
      also known as sialic acid-binding immunoglobulin-type lectin (Siglec-1)] and 
      porcine CD163 (pCD163) have been identified as the most important host entry 
      mediators that can fully coordinate PRRSV infection into macrophages. However, 
      recent isolates have not only shown a tropism for sialoadhesin-positive cells, 
      but also for sialoadhesin-negative cells. This observation might be partly 
      explained by the existence of additional receptors that can support PRRSV binding 
      and entry. In the search for new receptors, recently identified porcine Siglecs 
      (Siglec-3, Siglec-5 and Siglec-10), members of the same family as sialoadhesin, 
      were cloned and characterized. Only Siglec-10 was able to significantly improve 
      PRRSV infection and production in a CD163-transfected cell line. Compared with 
      sialoadhesin, Siglec-10 performed equally effectively as a receptor for PRRSV 
      type 2 strain MN-184, but it was less capable of supporting infection with PRRSV 
      type 1 strain LV (Lelystad virus). Siglec-10 was demonstrated to be involved in 
      the endocytosis of PRRSV, confirming the important role of Siglec-10 in the entry 
      process of PRRSV. In conclusion, it can be stated that PRRSV may use several 
      Siglecs to enter macrophages, which may explain the strain differences in the 
      pathogenesis.
FAU - Xie, Jiexiong
AU  - Xie J
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, B-9820 Merelbeke, Belgium.
FAU - Christiaens, Isaura
AU  - Christiaens I
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, B-9820 Merelbeke, Belgium.
FAU - Yang, Bo
AU  - Yang B
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, B-9820 Merelbeke, Belgium.
FAU - Breedam, Wander Van
AU  - Breedam WV
AD  - Unit for Medical Biotechnology, Medical Biotechnology Center, VIB, 
      Technologiepark 927, B-9052 Ghent, Belgium.
FAU - Cui, Tingting
AU  - Cui T
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, B-9820 Merelbeke, Belgium.
FAU - Nauwynck, Hans J
AU  - Nauwynck HJ
AD  - Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, 
      Salisburylaan 133, B-9820 Merelbeke, Belgium.
LA  - eng
PT  - Journal Article
DEP - 20170726
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Virus)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 3)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/genetics/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/genetics/metabolism
MH  - *Cloning, Molecular
MH  - Endocytosis
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory 
      Syndrome/genetics/*metabolism/physiopathology/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*metabolism
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Receptors, Virus/chemistry/genetics/*metabolism
MH  - Sequence Alignment
MH  - Sialic Acid Binding Ig-like Lectin 3/chemistry/genetics/*metabolism
MH  - Swine
PMC - PMC5656783
EDAT- 2017/07/26 06:00
MHDA- 2017/09/07 06:00
PMCR- 2017/07/26
CRDT- 2017/07/26 06:00
PHST- 2017/07/26 06:00 [pubmed]
PHST- 2017/09/07 06:00 [medline]
PHST- 2017/07/26 06:00 [entrez]
PHST- 2017/07/26 00:00 [pmc-release]
AID - 000859 [pii]
AID - 10.1099/jgv.0.000859 [doi]
PST - ppublish
SO  - J Gen Virol. 2017 Aug;98(8):2030-2042. doi: 10.1099/jgv.0.000859. Epub 2017 Jul 
      26.